Fig. 5

Swi6 nuclear export mutations result in increased cell size. (A) Cells lacking the Swi6 leucine-rich NES are larger than wild-type cells, but smaller than those completely lacking SWI6. Yeast cells containing genomic wild-type SWI6 (SWI6; wide hashed bars) or a genomic deletion of SWI6 (swi6Δ; narrow hashed bars) were transformed with empty plasmid (vector), plasmid expressing wild-type SWI6 (SWI6), or plasmid containing SWI6 with a mutation that changes L - > A at amino acids 250, 254, 257, and 258 (swi6ΔNES). Bars represent the average cell diameter in μm. Error bars represent s.e.m. Asterisks indicate significant differences (* p < 0.01). (B) Cells lacking both MSN5 and the Swi6 NES are larger than either single mutant. Wild-type, swi6Δ, msn5Δ, and swi6Δ msn5Δ cells lacking a Swi6 plasmid (gray; swi6Δ msn5Δ not reported due to synthetic lethality), or containing a plasmid expressing SWI6 (diagonally hashed) or swi6ΔNES (blue) were measured to determine average cell diameter. Bar heights represent average cell diameter in each sample. Error bars indicate s.e.m. for each. Asterisks indicate significant differences between two samples (p < 0.01). # indicates significant difference of sample from all others as examined using the Tukey multiple comparison of means (p < 0.0002)