Fig. 3
From: CREB1 regulates KPNA2 by inhibiting mir-495-3p transcription to control melanoma progression
CREB1 targeted the promoter of miR-495-3p. A TransmiR and ALGGEN were used to predict transcription factors regulating miR-495-3p. B A dual-luciferase reporter assay detected binding of CREB1 to the promoter of miR-495-3p. C Binding between CREB1 and the promoter of miR-495-3p was confirmed by the ChIP assay. D qRT‒PCR detected CREB1 levels in melanoma tissues. E qRT‒PCR detected CREB1 levels in melanoma cells, including A375, A2058, B16, and MUM2B cells. HEMa-LP cells served as the negative control. F qRT‒PCR detected CREB1 levels after A375 and B16 cells were transfected with oe-CREB1. The mean ± standard deviation represents data from three independent trials (n = 3). *p < 0.05, ** p < 0.01, *** p < 0.001 (n = 3)