Fig. 5
From: CREB1 regulates KPNA2 by inhibiting mir-495-3p transcription to control melanoma progression
miR-495-3p targeted KPNA2. A Bioinformatics analysis predicted the relationship between miR-495-3p and KPNA2, and a dual-luciferase reporter assay detected binding between miR-495-3p and KPNA2. B A dual-luciferase reporter assay detected binding of CREB1 to KPNA2. C qRT‒PCR detected KPNA2 levels in melanoma tissues. D qRT‒PCR detected KPNA2 levels in melanoma cells, including A375, A2058, B16, and MUM2B cells. HEMa-LP cells served as the negative control. E qRT‒PCR detected KPNA2 levels after A375 and B16 cells were transfected with oe-CREB1 and miR-495-3p mimics. The mean ± standard deviation represents data from three independent trials (n = 3). *p < 0.05, ** p < 0.01, *** p < 0.001 (n = 3)