Skip to main content

The HIV Tat protein affects processing of ribosomal RNA precursor



Inside the cell, the HIV Tat protein is mainly found in the nucleus and nucleolus. The nucleolus, the site of ribosome biogenesis, is a highly organized, non-membrane-bound sub-compartment where proteins with a high affinity for nucleolar components are found. While it is well known that Tat accumulates in the nucleolus via a specific nucleolar targeting sequence, its function in this compartment it still unknown.


To clarify the significance of the Tat nucleolar localization, we induced the expression of the protein during oogenesis in Drosophila melanogaster strain transgenic for HIV-tat gene. Here we show that Tat localizes in the nucleoli of Drosophila oocyte nurse cells, where it specifically co-localizes with fibrillarin. Tat expression is accompanied by a significant decrease of cytoplasmic ribosomes, which is apparently related to an impairment of ribosomal rRNA precursor processing. Such an event is accounted for by the interaction of Tat with fibrillarin and U3 snoRNA, which are both required for pre-rRNA maturation.


Our data contribute to understanding the function of Tat in the nucleolus, where ribosomal RNA synthesis and cell cycle control take place. The impairment of nucleolar pre-rRNA maturation through the interaction of Tat with fibrillarin-U3snoRNA complex suggests a process by which the virus modulates host response, thus contributing to apoptosis and protein shut-off in HIV-uninfected cells.


The HIV-1 Tat protein is an RNA binding protein essential for viral replication [1], which regulates productive and processive transcription from the HIV-1 long terminal repeat (LTR) by binding to the transactivation response (TAR) element [2]. Tat also affects several cellular functions by inducing angiogenesis [3, 4], cell proliferation and apoptosis [5]. Moreover, Tat regulates cytokine gene expression [5, 6], immune cell activation and may be secreted by HIV-1 infected cells, and thus act on neighboring cells [79]. Furthermore, by interacting with tubulin [10, 11], Tat affects mitosis and induces aneuploidies [12].

It has been shown that in both infected and uninfected cells, Tat also localizes in the nucleolus [13] and that the nucleolar accumulation of Tat occurs via the highly conserved stretch of basic amino acids that acts as a nucleolar localization signal (NoLS) [1417].

The nucleolus is a highly structured and dynamic organelle involved in the transcription and maturation of rRNA and ribosome biogenesis as well as in apoptosis and in cell cycle control [18]. Recently, various studies have suggested that the nucleolus plays a role in HIV-1 infections. First, lymphocytes isolated from infected patients show abnormal nucleolar structures [19]. Secondly, nucleolar localization of TAR impairs virus progression [20, 21].

A number of proteins encoded by RNA viruses, such as protein V of adenovirus [22], protein N of porcine virus [23] and HIV-1-Rev protein [24], are known to enter the nucleolus and to colocalize with nucleolar components suggesting that nucleolar localization is an important step in promoting viral replication [25]. While it is known that Rev multimerizes in the nucleolus and appears to play a role in the nucleoplasmic transport of viral unspliced RNAs [26]; the function of Tat in this nuclear structure is still unknown.

To gain insight into the functional significance of Tat nucleolar localization, we used a Drosophila melanogaster Tat transgenic strain [10, 12] and analysed the effect of this protein on ribosome biogenesis by expressing it (under the control of hsp70 promoter) during Drosophila oogenesis. Drosophila oogenesis is, in fact, the period of highest ribosome biosynthesis, and the genetic and molecular events (highly conserved from yeast to human) of this process have been extensively studied and described in detail [2729]. Furthermore, recent data indicate that Drosophila is a useful complementary system for studying HIV-1-related genes and proteins [10, 30, 31].

Here we show that Tat localizes with fibrillarin in nurse cell nucleoli, causes death and delayed development of the progeny and decreases the level of the 80S ribosome particles. This decrease appears to result from a Tat induced inhibition of pre-rRNA processing caused by the interaction of Tat with U3 snoRNA and fibrillarin, nucleolar components that are essential for the early steps of pre-rRNA processing. The results reported in this paper, provide the first evidence of the action of Tat on nucleolar functions and further our understanding of the molecular mechanism underlying Tat-mediated pathogenesis.


Tat expressed by heat shock treatment, colocalizes with fibrillarin in the nucleolus of Drosophila cells

Nucleolar localization of the HIV Tat protein has been extensively shown using several cellular systems [1317]. To shed light on the effects of the nucleolar localization of Tat, we determined whether it localizes in the nucleolus of tat transgenic Drosophila melanogaster cells, where its expressions is driven under the control of the heat shock promoter [10]. Since it is known that heat shock affects ribosome biogenesis and its effects terminate four hours later [32], we first investigated the time conditions that allow high expression of Tat in the absence of heat shock treatment effects (see Materials and Methods). To this end, we analyzed the expression of both Hsp70 and Tat proteins by Western blot analysis. Results in Fig. 1A show that the levels of Tat protein rise soon after heat shock treatment and continue for six hours during the ensuing treatment. In contrast, an increased level of Hsp70 protein appears between 30 minutes and two hours after treatment (Fig. 1B). Based on these results, all analyses were carried out by subjecting Drosophila flies to three heat shocks and by starting experiments six hours thereafter. Further to focusing on Tat nucleolar localization, Drosophila nurse cells were used since they are relatively large (due to polyploidy) and each contains several nucleoli. The images in Fig 1C show that Tat expressed in Drosophila transgenic flies localizes, as expected, both in the nucleoplasm and in the nucleolus, where it colocalizes with the nucleolar marker fibrillarin. This result supports the idea that the nucleolar localization signal works in Drosophila as well as mammalian cells.

Figure 1
figure 1

Expression of Tat and its colocalization with fibrillarin in Drosophila nurse cell nucleoli from Tat transgenic strain after heat shock treatment. Protein extract from Tat transgenic strain at different time-points, up to six hours after heat shock were analysed by western blot (A). Control (WG six hours after treatment) and recombinant Tat are shown. Expression of HSP70 protein in Tat transgenic flies, at different times after heat-shock treatment (+) is shown in B, as well as levels of HSP70, in flies not subjected to heat-shock (-). α-tubulin is shown as the control in both panels. Confocal images (C) from Drosophila nurse cells double labelled with anti-fibrillarin (green), and anti-Tat (red); merged image reveals the colocalization of Tat with fibrillarin in the nurse cell nucleoli after heat shock treatment.

Since the nucleolus is the site of ribosome RNA synthesis, we investigated whether Tat expression gives rise, in Drosophila, to phenotypes affecting ribosome biogenesis such as death and delayed development [28]. Therefore, to test whether the expression of Tat gives rise to similar phenotypes, we expressed this protein during Drosophila oogenesis and checked the progeny for mortality and developmental time. The data show that the expression of Tat causes significant increased mortality at embryonic and larval stages (Table 1). Moreover, developmental stages were delayed (4 days) compared with the control strain. These results indicate that Tat may affect ribosome biogenesis.

Table 1 Number of embryos, larvae and adults from Drosophila WG (control) and Tat strains after heat shock treatment. Results are means ± S.D. (n = 6); P< 0,001

Tat reduces the level of 80S ribosomes

Based on the above results, we tested whether Tat interferes with ribosome biogenesis by analysing the ribosome distribution profiles of both transgenic and control flies subjected to three heat shock treatments. Sucrose gradient profiles in Figure 2 show that, under these conditions, post mitochondrial supernatant (PMS) from Tat-transgenic flies (Fig. 2A right) contains a significantly lower amount of 80S ribosomes than comparable samples from control flies (Fig. 2A left). As expected, no significant difference was observed in strains not subjected to heat shock (Fig. 2B). Histograms represent the average area, obtained from 3 independent experiments, of the 80S ribosome peak. Significant differences (p < 0.01) were detected only in Tat-transgenic flies after heat shock treatment.

Figure 2
figure 2

Tat affects ribosomal profiles from Drosophila female extracts. Ribosomal profiles of control (WG) and transgenic (Tat-strain) strains, subjected (A) and not (B) to heat shock treatment (equal amount of O.D.260 were loaded). Histograms show quantification of 80S particle, (** p < 0.01).

Tat interferes with pre-rRNA processing patways

In order to assess whether Tat interferes with ribosome biogenesis at the nucleolar level, we investigated the effect(s) of Tat on pre-rRNA processing. In Drosophila, processing of the rRNA primary transcript (pre-rRNA) proceeds by two alternative (α and β) cleavage pathways [27, 29]. In the α processing pathway (shared by all Eucarya) the initial cleavage step removes the ETS, leaving a large molecule encompassing both the 18S and 28S rRNA sequences (intermediate a) (Fig. 3A). In the alternative β pathway, the primary transcript is cleaved inside the ITS region, at site 3, generating two processing intermediates; the first containing the 18S rRNA precursor (intermediate d), and the second containing the 28S rRNA precursor (intermediate b) (Fig. 3A). The effect of Tat on pre-rRNA processing was investigated by Northern blot analysis using total RNA extracted from Drosophila females of both transgenic and control strains subjected and not to heat shock treatment. The presence of unprocessed rRNA precursors in Tat-expressing cells was investigated using a DNA probe (probe I) which specifically hybridizes with the ITS1 5' region (Fig. 3A), thus allowing the identification of unprocessed pre-rRNA and both the a and d precursors. As Fig. 3B shows, hybridization of total RNA from Tat-expressing and control flies with the ITS1-specific probe clearly reveals accumulation of type d precursor only in Tat-expressing flies. Therefore, Tat expression in Drosophila seems to affect at least cleavage site 1. With the α pathway blocked, pre-rRNA processing should proceed mainly through pathway β, generating increasing amounts of intermediate d [29]. Indeed, hybridization with probe II shows higher levels of both b and d intermediate forms in the Tat expressing strain (Fig. 3C), suggesting that both pathways may be affected. The observation that Tat mainly interferes with site 1 cleavage was further confirmed by Northern blot experiments using a DNA probe (probe III) that specifically hybridizes with the 5' ETS region (Fig. 3A), thus allowing detection of accumulated intermediate d forms. As Fig. 3D shows, a significant increase of d form RNA occurs only in transgenic flies expressing Tat, which confirms that Tat affects at least cleavage site 1 impairing pre-rRNA maturation.

Figure 3
figure 3

Tat affects rRNA processing. (A): scheme of Drosophila rRNA precursor with cleavage sites corresponding to the two alternative processing pathways, α and β. Sequences of cleavages generating the two different pathways are highlighted under the scheme (see reference 29 for detail). Solid bars show probe positions. (B-D): Northern blot of RNA extract from control (WG) and transgenic strains (Tat-strain) with (+) or without (-) heat shock treatment. Probe I, derived from the ITS1 region and closed to cleavage site 2, was used in B. The same blot was hybridized using respectively probe II (C) derived from the ITS1 region including cleavage site 3 and probe III (D) derived from the ETS region, closed to cleavage site 1. The different immature products are indicated on the left (see scheme 4A). Tubulin is shown as loading control. Histograms represent the average from three independent experiments.

Tat binds to ETS-18S region of pre-rRNA

To understand whether Tat protein interacts with pre-rRNA, we performed EMSA using an RNA stretch comprising cleavage site 1 of ETS-18S rRNA (probe IV in Fig. 3A) as the probe. We incubated nuclear extracts from control and transgenic strains with probe IV after heat shock treatment. In contrast to samples from non-Tat expressing flies (Fig. 4A, lanes 2 and 3), incubation with extracts from Tat expressing flies resulted in considerable retardation of probe electrophoretic mobility (Fig. 4A, lanes 4 and 5). The shift was abolished after incubation the extracts with anti-Tat antibody (lanes 8 and 9) before adding the probe. To further confirm the specificity of the interaction extracts were incubated with a random RNA probe transcribed from cloning vector sequence (Fig. 4A, lanes 6 and 7) and with an unspecific structured probe (tRNA) (Fig. 4B). No shift was observed in any of these cases.

Figure 4
figure 4

Tat binds to the ETS-18S region. Electro mobility shift assay (EMSA) using as the probe the region corresponding to the ETS-18S pre-rRNA, containing cleavage site 1 (probe IV in Fig. 3A). (A): nuclear extracts from control (WG), and transgenic (Tat-strain) strains were incubated, after heat shock treatment, with the ETS-18S probe. Lane 1, free probe. In lanes 2 and 3, the probe was incubated with 0.5 and 3 μg of nuclear extract from control strain. In lanes 4 and 5 the probe was incubated with 0.5 μg and 3 μg of nuclear extract from transgenic strain. Unspecific probe, was incubated (lane 7) or not (lane 6) with nuclear extract from transgenic strain. In lane 8 and 9 nuclear extracts were pre-incubated with the anti-Tat antibody and then with the probe. (B): Nuclear extracts from control (WG), lanes 2–3, and transgenic (Tat-strain) strains, lanes 4–5, were incubated, after heat shock treatment, with an unrelated structured probe (tRNA). Probe alone is shown in lane 1.

Tat in vivo interacts with components involved in the first step of pre-rRNA processing

Fibrillarin is one of the essential components involved in pre-rRNA maturation. It localizes throughout the nucleolus and in the fibrillar region of mammalian nucleoli [33]. Since Tat colocalizes with fibrillarin in Drosophila transgenic strains (Fig. 1C), we next investigated whether Tat interferes with pre-rRNA processing by interacting with fibrillarin.

To this end, nuclear protein extracts were immunoprecipitated with anti-fibrillarin polyclonal antibodies and challenged with anti-Tat polyclonal antibodies (Fig. 5A). The specific signal was detected in the Tat-expressing strain (lane 2), whereas, as expected, no signal was observed in the control strain (lane 1). Similarly, when nuclear extracts were immunoprecipitated with anti-Tat antibodies and then challenged with anti-fibrillarin antibodies (Fig. 5B), the immunoreactive band was detected only in precipitates from the Tat-expressing strain (lane 2). Interestingly the detection was abolished when samples were preincubated with RNase before proceeding with the immunoprecipitation (Fig. 5B, bottom lanes). Since fibrillarin is known to participate in RNA cleavage in association with U3 snoRNA, we tested whether Tat forms immunoprecipitates with U3 snoRNA [34, 35]. Accordingly, we incubated nuclear extracts with Tat-polyclonal antibodies and then monitored the presence of a Tat-U3 complex by Northern blotting using a U3 specific oligonucleotide as a probe. Results in Fig. 5C (lanes 1 and 2), clearly show that U3 is associated with Tat in immunoprecipitates from a transgenic strain expressing Tat. Specificity of the immunoprecipitation was confirmed performing the experiment with an unrelated antibody (anti Tyrosine hydroxylase). In such case no specific Tat signal was detected (Fig. 5D). On the whole, the results in Fig. 5 suggest that the association of Tat with fibrillarin and U3 snoRNA impairs pre-rRNA processing.

Figure 5
figure 5

Tat interaction with the fibrillarin-U3 snoRNA complex. Extracts from control flies (WG) (lane 1), and Tat-transgenic flies (Tat-strain) (lane2), following heat-shock treatment. (A) From top to bottom: nuclear lysate probed with anti-fibrillarin antibody; anti-fibrillarin immunoprecipitate probed with anti-fibrillarin antibody; anti-fibrillarin immunoprecipitate probed with anti-Tat; antibody; heavy chain (h.c.) are shown as loading control. (B) From top to bottom: Nuclear lysate probed with anti-Tat; anti-Tat immunoprecipitate probed with anti-Tat antibody; anti-Tat immunoprecipitate probed with anti-fibrillarin antibody; nuclear lysate immunoprecipitated with anti Tat antibody, after RNase treatment, and than probed with anti fibrillarin antibody; antibody heavy chain (h.c.) are shown as loading control. (C) Northern blotting of nuclear extract immunoprecipitated with anti-Tat and probed with U3 specific oligonucleotide. (D) Immunoprecipitation with an unrelated antibody. From top to bottom: nuclear lysate probed with anti Tat antibody; nuclear lysate immunoprecipitated with anti tyrosine hydroxylase (T.H.) and probed with anti Tat antibody; antibody heavy chain (h.c.) are shown as loading control.


In this study, we show that Tat expressed in Drosophila colocalizes with fibrillarin in nurse cell nucleoli, produces a significant decrease of cytoplasmic ribosomes and affects cleavage sites during RNA precursor maturation.

In Jurkat T cells, it has been shown that Tat localizes throughout the nucleolus and, by electron microscopy, has been visualized in the dense fibrillar (DFC) and granular (GC) nucleolar components [33] where pre-rRNA processing mainly occurs. Therefore, we surmise that Tat affects ribosomal RNA maturation by interfering with post-trascriptional modifications and/or interacting with molecules involved in cleavage events. The result in Fig. 5 indicates that Tat immunoprecipitates with fibrillarin and with small nucleolar U3 RNA. The interaction of Tat with fibrillarin seems to be mediated by the presence of RNA based components cause it is abolished after RNase treatment. However we cannot exclude that RNase treatment compromise the integrity of the complex, and than impair protein-protein interaction.

Fibrillarin (highly conserved among eukaryotes) is a component of C/D box small nucleolar ribonucleoproteins (snoRNPs) and directs 2'-O-methylation of rRNA. In association with U3, U8, and U13 small nuclear RNAs, it is involved in the first step of pre-rRNA processing [34, 35]. The processing and modification of ribosomal pre-RNA in the nucleoli of eukaryotic cells, is accomplished by a large number of small nucleolar RNAs (snoRNAs) complexed with proteins in ribonucleoproteic particles (snoRNPs) that are essential for rRNA chemical modifications, such as methylation and pseudouridylation [36, 37]. Defects in some of these components have been associated with defects in pre-rRNA processing [29, 38]. Thus, Tat can inhibit or delay the processing of the pre-rRNA by subtracting fibrillarin-snoU3 complex from the initial cleavage site. Alternatively Tat could interact: i) with specific RNAs and drag along fibrillarin and other core components of the snoRNP (such as Nop56, Nop58, 15.5 K); ii) with proteins that interact with snoRNPs (such as Nopp140 or nucleolin); iii) or directly with the rRNA. This should affect fibrillarin functions in cells harbouring Tat. Interestingly, a similar mechanism has been suggested for Nidoviruses, which are able to traslocate the nucleocapside (N) protein into the nucleolus, where it interacts with fibrillarin, by an RNA mediated mechanism and possibly affects pre-RNA processing [23].

It is known that in mammalian cells, cell cycle regulation is related to ribosome biogenesis at nucleolar level [39], and that inhibition of pre-rRNA processing directs the cells toward apoptosis. Data report that mutation in nucleolar components results in pre-rRNA maturation defects and apoptosis. It is the case of mutation of minifly gene (which encodes an ubiquitous nucleolar protein (Mfl) in Drosophila [29]), and mutation in the gene encoding ribosomal RPS19 protein in humans [38]. Therefore, one of the molecular mechanisms by which both HIV infected and uninfected cells undergo apoptosis may depend on the pre-rRNA processing inhibition mediated by Tat.

Modulating cellular protein synthesis and host cell metabolism is a strategy common in many viruses to induce cellular shut-off [40]. A mechanism by which HIV-1 induces cellular shut-off seems to depend on the binding of the second coding exon of Tat to the translation elongation factor 1-delta (EF-1δ) [41]. As we observed, the inhibition of pre-rRNA processing mediated by Tat leads to a decreased amount of 80S ribosome particles. The decrease of the cytoplasmic ribosomes should result in reduced protein synthesis of the host cell. Therefore, the inhibition of pre-rRNA processing caused by Tat delineates a novel mechanism for HIV-1 mediated cellular shut-off.


In the present work we show for the first time that Tat, in the nucleolus, affects ribosome rRNA maturation. This suggests that Tat manipulates basic cellular processes in order to control ribosomal biogenesis.

An intriguing hypothesis is that Tat, secreted by HIV-1 infected cells, acts on the nucleolar compartment of neighbouring uninfected cells, inducing apoptosis and consequent loss of immune competence, which is the main cause of AIDS pathogenesis.


Drosophila stock and transgenic line

Fly stocks: w67c23 used as control (named WG) and WG hsp:Tat transgenic line (named Tat-strain) [10].

Expression of HIV-Tat protein by heat shock treatment

In all experiments, Tat expression (under the control of hsp70 promoter) was induced by subjecting Drosophila females to heat-shock treatment for 30' at 37°C for three days. To test the viability and developmental time of strain expressing Tat, Drosophila virgin females (1 day old) of both transgenic and control lines were subjected to heat shock treatments and then mated. For each strain laid embryos were collected and counted at 24 and 48 hours. The time for different developmental stages (from embryo to larva and from larva to pupa) was measured. The experiment was repeated five times. Statistical analysis was performed with Graph-pad Prism software. To determine the time conditions of Tat expression, synchronized Drosophila females, one day old, were subjected to the heat shock procedure described above and collected at different times at 25°C. The protein extracts were analysed by Western blotting to detect Tat and Hsp70 proteins.

Immunofluorescence microscopy

Ovaries, extracted after 6 hours following the last heat shock, in the presence of 1× modified Robb's medium (MRM) [42], were dissected in PBS buffer containing 5% glycerol, incubated with 100% methyl alcohol for 10' at room temperature and then 30' at -20°C. The same treatment was repeated with 100% acetone. After washing with PBS containing 3% BSA, the ovaries were incubated overnight at 4°C with 40% goat serum and then with rabbit anti-Tat (kindly provided by E. Loret) and/or mouse anti-fibrillarin (abcam 38F3 Cambridge, UK) both diluted 1:700 overnight at 4°C. Secondary antibodies conjugated respectively with rhodamine and FITC (Cappel) were both diluted 1:500. Samples were analysed with a Leica confocal.

Western blotting and Immunoprecipitation

To detect the expression of Tat in Tat-transgenic Drosophila, 1-day old females were subjected to heat shock treatment (see section above), and protein extracts were fractionated by 15% SDS-polyacrilamide gel (PAGE) elecrophoresis and transferred to PVDF sheets (Biorad). Membranes were incubated in 10% of no fat dry milk (Biorad) in NET buffer [43] overnight at 4°C. After incubation, the sheets were washed in NET buffer and then incubated with both anti-Tat antibody at a dilution 1:2000 for 4 h and Hsp70 antibody (1: 1000 dilution; Stressgene), at room temperature. Blots were then washed and incubated with secondary antibody (goat anti-rabbit) conjugated to horseradish-peroxidase (Biorad, 1:10,000). The reaction was visualised by incubation with ECL chemiluminescence reagent (Biorad). For immunoprecipitation experiments, an equal amount of nuclear protein extracts (as further described in the EMSA assay) [44] were immunoprecipitated with anti-fibrillarin antibody (kindly provided by I. Bozzoni), with anti-Tat antibody or with anti Tyrosine hydroxylase antibody (Chemicon), using protein A/G plus-agarose (Santa Cruz) as recommended by the manufacturer. For RNase treatment extracts were incubated in presence of 40 μg/ml of RNaseA (Fermentas). Precipitated proteins were resolved by 12% SDS-PAGE and immunoblotted with anti-Tat or anti-fibrillarin antibodies (Abcam, Cambridge). Secondary antibodies used to detect Tat and fibrillarin were goat anti-rabbit (Biorad, 1:10,000) and goat anti-mouse (Biorad, 1:10,000), respectively. For Northern blot experiments immunoprecipitates were resolved by 7% acrylamide gel, containing 8 M urea and TBE buffer [43] and filters hybridised with U3 oligonucleotide (5'CGGGATCCACCACTCAGAATTCGCTCTATC CG-3'), complementary to 3' end of Drosophila U3 snoRNA (Dm-818/Dm-830).

Sucrose gradient sedimentation

Ribosomal profiles were analysed using post-mitochondrial supernatant (PMS) [45]. Briefly, 50 Drosophila females were homogenized in 0.5 ml of buffer (50 mM Tris, pH 7.4, 80 mM KCl, 10 mM MgCl2, and 0.25 M sucrose) at 0°C. After 30' centrifugation at 13000 rpm, 4°C, supernatant was collected and nucleic acid concentration was measured by spectrophotometric determination (Beckman DU 530). An equal A260 of PMS was loaded on 10% to 34% linear sucrose gradient containing: 50 mM Tris pH 7.4, 80 mM KCl, 10 mM MgCl2. Samples were centrifuged at 35.000 rpm at 4°C for 1 h, 45' (Beckman LE-80 K, rotor SW41) and analysed by density gradient fractionator (Isco Model UA-5). Statistical analysis was performed with Graph-pad Prism software.

Northern blot analysis

Total RNA was extracted from 50 Drosophila females after heat shock treatment. The insects were homogenized in 0.6 ml of lysis buffer (7 M urea, 0.35 M NaCl, 0.1 M Tris-Hl pH 8, 10 mM EDTA pH 8, 2% SDS). After phenol extraction RNA was precipitated first with ethanol and than with 2 M LiCl. For Northern blot analysis [43] 10 μg of total RNA were electrophoresed and transferred to Nytran Super Charge (Schleicher and Schuell) filters for hybridisation.

The following probes were used: Probe I corresponds to oligonucleotide 5'-GTTAAAATCTTTTTATGAGGTTGCCAAGCCCCACAC-3' and probe II corresponds to oligonucleotide 5'-CACCATTTTACTGGCATATATCAATTCCTTCAATAAATG-3' (Fig. 4A). Both were phosphorilated with [γ-P32] at 5' terminal using T4 Kinase (Roche). Probe III (to identify 5'ETS) was amplified by PCR from genomic DNA of Drosophila melanogaster (using as primers ETS2up 5'CCCGGATCCGTCAAGTTTCTATTATACATAGA3' and ETS2dw 5'-GCTCTAGATATAACATAAAACCGAGCGCACATGATAATTCTT-3') and phosphorilated with [α-P32] by random priming (Boehringer). Probe for tubulin was generated with random priming kit (Roche). Three independent experiments were performed for each probe. Bands were quantified with a phosphoimager (Packard) and graphs plotted with Graph-pad Prism software.

Electro mobility shift assay (EMSA)

800 nucleotides of the ETS-18S region (probe IV) were obtained by PCR from genomic Drosophila DNA using the following primers: 5'-ETS2up 5'-ACGGATCCGGTAGGCAGTGGTTG-3' and 18Sdw 5'-ACGCGTCGACTAATGATCCTTCCGGAGG-3'. The sequence cloned into pBSK was transcripted with T7 polymerase and labelled with [α-UTP32]. Unspecific probe, (900 nucleotides) was obtained from linearized pBSK, following transcription. tRNA labelled with [γ-ATP32] was used as the unspecific structured probe.

Nuclear extracts from 30 females, subjected and not to heat shock treatment, were obtained using the following procedure: flies were homogenized at 0°C in 0.5 ml of buffer A (1 mM Sucrose, 0.5 mM MgCl2, 20 mM NaCl and 20 mM Tris pH 7.4) containing 2 mM DTT, 1 mM PMSF, 50 μl of the protease inhibitor cocktail 7× (Amersham) and 20 μl of RNase inhibitor (5U/μl) (Sigma). Total lysate was first centrifuged 5' at 800 rpm 4°C to remove cellular debris and then 5' at 6000 rpm 4°C. Pellet, containing crude nuclear fraction, was homogenized in 0.5 ml PBS containing 0.2 M NaCl and 0.05% nonided-P40 and incubated 10' at 0°C. Nuclear extracts were then separated from insoluble fractions after centrifugation at 13.000 rpm 4°C. Nuclear extracts from Drosophila females were incubated in binding buffer (50 mM Tris-HCl pH 7.9, 200 mM NaCl, 8% glycerol), poly dI-dC (0.1 μg/μl), RNase inhibitor (5U/μl), at 0° for 10'. Then, samples were incubated with or without anti-Tat antibody at 0° C for 15' and then with 5 fmol of labelled probe (specific activity: 1.5 × 109cpm/μg) at 0°C for 10'. Preparations were resolved by 4% polyacrylamide gel in non denaturating conditions, transferred onto 3 MM paper, and exposed overnight to phosphoimager (Packard).



human immunodeficiency virus, type 1


transcriptional activator


transactivation response


long terminal repeat


heat shock protein.


  1. Liang C, Wainberg MA: The role of Tat in HIV-1 replication: an activator and/or a suppressor?. AIDS Rev. 2002, 4: 41-49.

    PubMed  Google Scholar 

  2. Loret EP, Georgel P, Johnson WC, Ho PS: Circular dichroism and molecular modeling yield a structure for the complex of human immunodeficiency virus type 1 trans-activation response RNA and the binding region of Tat, the trans-acting transcriptional activator. Proc Natl Acad Sci USA. 1992, 89: 9734-9738. 10.1073/pnas.89.20.9734.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Mitola S, Soldi R, Zanon I, Barra L, Gutierrez MI, Berkhout B, Giacca M, Bussolino F: Identification of specific molecular structures of human immunodeficiency virus type 1 Tat relevant for its biological effects on vascular endothelial cells. J Virol. 2000, 74 (1): 344-353.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Benelli R, Barbero A, Ferrini S, Scapini P, Cassatella M, Bussolino F, Tacchetti C, Noonan DM, Albini A: Human immunodeficiency virus transactivator protein (Tat) stimulates chemotaxis, calcium mobilization, and activation of human polymorphonuclear leukocytes: implications for Tat-mediated pathogenesis. J Infect Dis. 2000, 182: 1643-1651. 10.1086/317597.

    Article  CAS  PubMed  Google Scholar 

  5. Barillari G, Ensoli B: Angiogenic effects of extracellular human immunodeficiency virus type 1 Tat protein and its role in the phatogenesis of AIDS-associated Kaposi's sarcoma. Clin Microbiol Rev. 2002, 15: 310-326. 10.1128/CMR.15.2.310-326.2002.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Contreras X, Bennasser Y, Chazal N, Moreau M, Leclerc C, Tkaczuk J, Bahraoui E: Human immunodeficiency virus type 1 Tat protein induces an intracellular calcium increase in human monocytes that requires DHP receptors: involvement in TNF-alpha production. Virology. 2005, 332: 316-328. 10.1016/j.virol.2004.11.032.

    Article  PubMed  Google Scholar 

  7. Frankel AD, Pabo CO: Cellular uptake of the tat protein from human immunodeficiency virus. Cell. 1988, 55: 1189-1193. 10.1016/0092-8674(88)90263-2.

    Article  CAS  PubMed  Google Scholar 

  8. Tyagi M, Rusnati M, Presta M, Giacca M: Internalization of HIV-Tat requires cell surface heparan sulfate proteoglycans. J Biochem. 2001, 276: 3254-3261.

    CAS  Google Scholar 

  9. Vendeville A, Rayne F, Bonhoure A, Bettache N, Montcourrier P, Beaumelle B: HIV-1 Tat enters T cells using coated pits before translocating from acidified endosomes and eliciting biological responses. Mol Biol Cell. 2004, 15: 2347-2360. 10.1091/mbc.E03-12-0921.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Battaglia PA, Zito S, Macchini A, Gigliani FA: Drosophila model of HIV-1 TAT-related pathogenicity. J Cell Sci. 2001, 114: 2787-2794.

    CAS  PubMed  Google Scholar 

  11. Chen D, Wang M, Zhou S, Zhous Q: HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim. EMBO J. 2002, 21: 6801-6810. 10.1093/emboj/cdf683.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Battaglia PA, Ponti D, Naim V, Venanzi S, Psaila R, Gigliani F: The HIV-Tat protein induces chromosome number aberrations by affecting mitosis. Cell Motil Cytoskel. 2005, 61: 129-136. 10.1002/cm.20070.

    Article  CAS  Google Scholar 

  13. Siomi H, Shida H, Maki M, Hatanaka M: Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization. J Virol. 1990, 64 (4): 1803-1807.

    PubMed Central  CAS  PubMed  Google Scholar 

  14. Ruben S, Perkins A, Purcell R, Joung K, Sia R, Burghoff R, Haseltine WA, Rosen CA: Structural and functional characterization of human immunodeficiency virus tat protein. J Virol. 1989, 63 (1): 1-8.

    PubMed Central  CAS  PubMed  Google Scholar 

  15. Luznik L, Martone ME, Kraus G, Zhang Y, Xu Y, Ellisman MH, Wong-Staal F: Localization of human immunodeficiency virus Rev in transfected and virus-infected cells. AIDS Res Hum Retroviruses. 1995, 11 (7): 795-804.

    Article  CAS  PubMed  Google Scholar 

  16. Li YP: Protein B23 is an important human factor for the nucleolar localization of the human immunodeficiency virus protein Tat. J Virol. 1997, 71 (5): 4098-4102.

    PubMed Central  CAS  PubMed  Google Scholar 

  17. Stauber RH, Pavlakis GN: Intracellular trafficking and interactions of the HIV-1 Tat protein. Virology. 1998, 252: 126-136. 10.1006/viro.1998.9400.

    Article  CAS  PubMed  Google Scholar 

  18. Gerbi SA, Borovjagin AV, Lange TS: The nucleolus: a site of ribonucleoprotein maturation. Curr Opin Cell Biol. 2003, 15: 318-325. 10.1016/S0955-0674(03)00049-8.

    Article  CAS  PubMed  Google Scholar 

  19. Galati D, Paiardini M, Cervasi B, Albrecht H, Bocchino M, Costantini A, Montroni M, Magnani M, Piedimonte G, Silvestri G: Specific changes in the posttranslational regulation of nucleolin in lymphocytes from patients infected with human immunodeficiency virus. J Infect Dis. 2003, 188: 1483-1491. 10.1086/379249.

    Article  PubMed  Google Scholar 

  20. Michienzi A, Cagnon L, Bahner I, Rossi JJ: Ribozyme-mediated inhibition of HIV 1 suggests nucleolar trafficking of HIV-1 RNA. Proc Natl Acad Sci USA. 2000, 97: 8955-8960. 10.1073/pnas.97.16.8955.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Michienzi A, Li S, Zaia JA, Rossi JJ: A nucleolar TAR decoy inhibitor of HIV-1 replication. Proc Natl Acad Sci USA. 2002, 99: 14047-14052. 10.1073/pnas.212229599.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Matthews DA, Russell WC: Adenovirus core protein V is delivered by the invading virus to the nucleus of the infected cell and later in infection is associated with nucleoli. J Gen Virol. 1998, 79 (Pt 7): 1671-1675.

    Article  CAS  PubMed  Google Scholar 

  23. Yoo D, Wootton SK, Li G, Song C, Rowland RR: Colocalization and interaction of the porcine artevirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. J Virol. 2003, 77: 12173-12183. 10.1128/JVI.77.22.12173-12183.2003.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Ristea S, Dobbelstein M, Roth J: Rev protein of human immunodeficiency virus type 1 and cellular exportin 1 protein relocalize each other to a subnucleolar structure. AIDS Res Hum Retrov. 2000, 16: 857-865. 10.1089/08892220050042792.

    Article  CAS  Google Scholar 

  25. Hiscox J: RNA viruses: hijacking the dynamic nucleolus. Nat Rev Microbiol. 2007, 5: 119-127. 10.1038/nrmicro1597.

    Article  CAS  PubMed  Google Scholar 

  26. Daelemans D, Costes SV, Cho EH, Erwin-Cohen RA, Lockett S, Pavlakis GN: In vivo HIV-1 Rev multimerization in the nucleolus and cytoplasm identified by fluorescence resonance energy transfer. J Biol Chem. 2004, 279: 50167-50175. 10.1074/jbc.M407713200.

    Article  CAS  PubMed  Google Scholar 

  27. Long EO, Dawid IB: Alternative pathways in the processing of ribosomal RNA precursor in Drosophila melanogaster. J Mol Biol. 1980, 138: 873-888. 10.1016/0022-2836(80)90070-4.

    Article  CAS  PubMed  Google Scholar 

  28. Kay MA, Jacobs-Lorena M: Developmental genetics of ribosome synthesis in Drosophila. Trends Genet. 1987, 3: 347-351. 10.1016/0168-9525(87)90295-2.

    Article  CAS  Google Scholar 

  29. Giordano E, Peluso I, Senger S, Furia M: minifly, a Drosophila gene required for ribosome biogenesis. J Cell Biol. 1999, 144: 1123-1133. 10.1083/jcb.144.6.1123.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  30. Leulier F, Marchal C, Miletich I, Limbourg-Bouchon B, Benarous R, Lemaitre B: Directed expression of the HIV-1 accessory protein Vpu in Drosophila fat-body cells inhibits Toll-dependent immune responses. EMBO Rep. 2003, 4: 976-981. 10.1038/sj.embor.embor936.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  31. Spresser CR, Carlson KA: Drosophila melanogaster as a complementary system for studying HIV-1-related genes and proteins. J Neurosci Res. 2005, 80: 451-455. 10.1002/jnr.20460.

    Article  CAS  PubMed  Google Scholar 

  32. Bell J, Neilson L, Pellegrini M: Effect of heat schock on ribosome synthesis in Drosophila melanogaster. Mol Cell Biol. 1988, 8 (1): 91-95.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. Marasco WA, Szilvay AM, Kalland KH, Helland DG, Reyes HM, Walter RJ: Spatial association of HIV-1 tat protein and the nucleolar transport protein B23 in stably transfected JurkatT-cells. Arch Virol. 1994, 139: 133-154. 10.1007/BF01309460.

    Article  CAS  PubMed  Google Scholar 

  34. Sharma K, Tollervey D: Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing. Mol Cell Biol. 1999, 19 (9): 6012-6019.

    PubMed Central  CAS  PubMed  Google Scholar 

  35. Newton K, Petfalski E, Tollervey D, Caceres JF: Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 2003, 23: 8519-8527. 10.1128/MCB.23.23.8519-8527.2003.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  36. Tollervey D, Lehtonen H, Jansen R, Kern H, Hurt EC: Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. Cell. 1993, 72: 443-457. 10.1016/0092-8674(93)90120-F.

    Article  CAS  PubMed  Google Scholar 

  37. Tollervey D, Kiss T: Function and synthesis of small nucleolar RNAs. Curr Opin Cell Biol. 1997, 9: 337-342. 10.1016/S0955-0674(97)80005-1.

    Article  CAS  PubMed  Google Scholar 

  38. Choesmel V, Bacqueville D, Rouquette J, Noaillac-Depeyre J, Fribourg S, Cretien A, Leblanc T, Tchernia G, Da Costa L, Gleizes PE: Impaired ribosome biogenesis in Diamond-Blackfan anemia. Blood. 2007, 109: 1275-1283. 10.1182/blood-2006-07-038372.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  39. Ruggero D, Pandolfi PP: Does the ribosome translate cancer?. Nat Rev Cancer. 2003, 3: 179-192. 10.1038/nrc1015.

    Article  CAS  PubMed  Google Scholar 

  40. Thompson SR, Sarnow P: Regulation of host cell translation by viruses and effects on cell function. Curr Opin Microbiol. 2000, 3: 366-370. 10.1016/S1369-5274(00)00106-5.

    Article  CAS  PubMed  Google Scholar 

  41. Xiao H, Neuveut C, Benkirane M, Jeang KT: Interaction of the second coding exon of Tat with human EF-1 delta delineates a mechanism for HIV-1-mediated shut-off of host mRNA translation. Biochem Bioph Res Com. 1998, 244: 384-389. 10.1006/bbrc.1998.8274.

    Article  CAS  Google Scholar 

  42. Matthies HJ, McDonald HB, Goldestein LS, Theurkauf WE: Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein. J Cell Biol. 1996, 134: 455-464. 10.1083/jcb.134.2.455.

    Article  CAS  PubMed  Google Scholar 

  43. Sambrook J, Fritsch EF, Maniatis T: Molecular cloning. A Laboratory Manual. 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2

    Google Scholar 

  44. Dignam JD, Martin PL, Shastry BS, Roeder RG: Eukaryotic gene transcription with purified components. Method Enzymol. 1983, 101: 582-598.

    Article  CAS  Google Scholar 

  45. Sripati CE, Warner JR: Isolation, characterization and translation of mRNA from yeast. Method Cell Biol. 1978, 20: 61-81.

    Article  CAS  Google Scholar 

Download references


We thank P. Cammarano for critical reading of the manuscript and for his helpful suggestions and P. Londei for her invaluable assistance with sucrose gradient. We also thank E.P. Loret and I. Bozzoni who kindly provided the antibodies used in this work.

This research was supported by: the Italian Ministero della Sanita' (AIDS project) and by a contribution from the Istituto Pasteur-Fondazioni Cenci Bolognetti Università di Roma.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Donatella Ponti.

Additional information

Authors' contributions

DP conceived and performed most of the studies, MT performed northern blot analysis, GCB performed confocal analysis, FG and PAB were involved in sponsoring the project and manuscript preparation. All the authors have read and approved the final manuscript.

Authors’ original submitted files for images

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Ponti, D., Troiano, M., Bellenchi, G.C. et al. The HIV Tat protein affects processing of ribosomal RNA precursor. BMC Cell Biol 9, 32 (2008).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: