Skip to main content
Download PDF

Identification and characterization of the LRR repeats in plant LRR-RLKs

BMC Molecular and Cell Biology volume 22, Article number: 9 (2021) Cite this article

Abstract

Background

Leucine-rich-repeat receptor-like kinases (LRR-RLKs) play central roles in sensing various signals to regulate plant development and environmental responses. The extracellular domains (ECDs) of plant LRR-RLKs contain LRR motifs, consisting of highly conserved residues and variable residues, and are responsible for ligand perception as a receptor or co-receptor. However, there are few comprehensive studies on the ECDs of LRR-RLKs due to the difficulty in effectively identifying the divergent LRR repeats.

Results

In the current study, an efficient LRR motif prediction program, the “Phyto-LRR prediction” program, was developed based on the position-specific scoring matrix algorithm (PSSM) with some optimizations. This program was trained by 16-residue plant-specific LRR-highly conserved segments (HCS) from LRR-RLKs of 17 represented land plant species and a database containing more than 55,000 predicted LRRs based on this program was constructed. Both the prediction tool and database are freely available at http://phytolrr.com/ for website usage and at http://github.com/phytolrr for local usage. The LRR-RLKs were classified into 18 subgroups (SGs) according to the maximum-likelihood phylogenetic analysis of kinase domains (KDs) of the sequences. Based on the database and the SGs, the characteristics of the LRR motifs in the ECDs of the LRR-RLKs were examined, such as the arrangement of the LRRs, the solvent accessibility, the variable residues, and the N-glycosylation sites, revealing a comprehensive profile of the plant LRR-RLK ectodomains.

Conclusion

The “Phyto-LRR prediction” program is effective in predicting the LRR segments in plant LRR-RLKs, which, together with the database, will facilitate the exploration of plant LRR-RLKs functions. Based on the database, comprehensive sequential characteristics of the plant LRR-RLK ectodomains were profiled and analyzed.

Background

To adapt to sessile lifestyles, plants need to sense various signals from the outside world in response to various environmental changes. Some plants have evolved to meet this challenge by receiving these signals via cellular membrane-localized receptor-like kinases (RLKs) [1,2,3,4]. The largest family of such receptors is termed leucine-rich-repeat (LRR) RLKs, which are involved in multiple developmental processes as well as disease resistances [4, 5]. LRR-RLKs are composed of an extracellular domain (ECD), which is responsible for ligand binding, a single membrane-spanning helix (TM), and a cytoplasmic kinase domain (KD) [4]. Typically, the plant LRR-RLK family is classified into 15–20 subgroups (SGs) based on phylogenetic analysis of the KDs and is denoted according to the SGs in Arabidopsis (Arabidopsis thaliana) LRR-RLKs (numbered with Roman numerals) [2, 6,7,8,9]. Although the classification of the LRR-RLK genes tends to rely on the phylogenetic analysis of the KDs due to the ambiguous alignment of the ECDs, similar structural arrangement patterns of the ECDs are often observed in most SGs [10]. In addition, in flowering plants, a more extensive selection pressure is imposed on ECDs than on KDs or TM in order to adapt to more sophisticated ligands recognition [5, 7, 11, 12].

LRRs share a common structure of 20–43 continuous residues uncommonly rich in the hydrophobic amino acid leucine [13]. Seven distinct LRR subfamilies have been identified, where the LRRs in LRR-RLKs share plant-specific consensus sequences (CS) such as LxxLxLxxNxL(s/t) GxLPxxLxxLxx (“L” refers to a hydrophobic amino acid, “N” refers to an asparagine, threonine, serine or cysteine, and “x” refers to variable residue) [14, 15]. Recently resolved structures reveal that the highly conserved region “LxxLxLxxN” in LRRs tend to assemble into a curved parallel β-sheet lining the inner circumference of their solenoid structure and the highly conserved region “L(s/t)GxLP” formed the plant-specific second β-strand which forced the LRR stacks out of a plain and into a rod, curve, and eventually superhelical assembly [14,15,16]. Therefore, the 16-residue segment “LxxLxLxxNxL(s/t)GxLP” could be taken as the plant-specific highly conserved segment (HCS). Moreover, according to the reported LRR-RLK-ligand complexes, the residues of the inner side of the ECDs are crucial for proper functioning of LRR-RLKs, as the inner surfaces bound the ligands to supply a platform for recruiting co-receptors to activate a signaling pathway in a structure complementary way [16,17,18]. Moreover, plant LRR-RLKs usually harbor heavy N-glycosylation modifications, which tend to located at the canonical asparagine-linked (Asn/N-) glycosylation sites, NxS/T sequons (x ≠ P) [19,20,21]. The N-glycosylation modifications are believed to contribute to the proper folding, trafficking and biological functioning of LRR-RLKs [19, 21,22,23]. Therefore, the efficient prediction of the extracellular LRR motifs and sequentially comprehensive analysis of ECDs for plant LRR-RLKs will benefit their functional characterization and binding site analysis.

To predict LRR regions, many methods are available that are based on the hidden Markov model (HMM) or sequence alignment with previously known LRRs, such as SMART [13], Pfam [24], which cannot effectively predict the most divergent LRRs in a given sequence [25]. Recently, two methods, LRRfinder [26] and LRRsearch [25], were conducted based on the position-specific scoring matrix algorithm (PSSM); these methods were proved to be powerful tools in predicting LRR motifs. The fact that LRRfinder performed well in Toll-like receptors (TLRs), whereas LRRsearch performed well in cytoplasmic NOD-like receptors (NLRs) [25] indicates that the efficiency of the PSSM based method strongly relies on the training datasets.

In the current study, I developed the “Phyto-LRR prediction” program to identify plant-specific LRR motifs with high efficiency using the PSSM algorithm, which was trained by the plant-specific 16-residue LRR-HCSs with some optimizations. Based on this program, more than 55,000 LRR motifs from 3987 protein sequences with LRRs, TM and KDs from 17 represented fully sequenced land-plant genomes were detected and stored in the database. Those with signal peptides were then classified into 18 SGs according to the maximum-likelihood phylogenetic analysis of the KD sequences and the LRRs arrangement in the ECDs was determined. Different from the remaining SGs, SG_x had two clusters of LRR numbers and density in the ECDs, which were then denoted as SG_x_1* and SG_x_2*, respectively, based on further phylogenetic analysis of SG_x. According to the database, some characteristics of the LRR motifs in each SG of the LRR-RLKs were examined, such as the density of the LRRs, the solvent accessibility, the variable residues, and the N-glycosylation sites, revealing a comprehensive profile of the plant LRR-RLK ECDs.

Results

The construction of the Phyto-LRR database

In total, 3987 protein sequences containing LRR(s), TM, and a KD from 17 represented embryophyte genomes were extracted for LRR motif prediction (see Methods), including four monocot genomes, ten dicot genomes, the liverwort Marchantia polomorpha, the moss Physcomitrella patens, and the spikemoss Selaginella moellendorffii. The species names, five-digit codes and the number of sequences extracted are listed in Table 1. From the ECD sequences of these protein sequences, 55,457 LRR motifs were predicted by the Phyto-LRR prediction program (Fig. 1; see Methods) and saved in the Phyto-LRR database (both the prediction tool and the database can be freely accessed at http://phytolrr.com for website usage and at http://github.com/phytolrr for local usage). The accuracy of this program was determined by comparing its predicted outcomes with LRRs identified in the crystal structures. As shown in Table 2, the Phyto-LRR prediction program performed better than SMART and Pfam, which are available online tools based on HMM and/or sequence alignment. For LRR-RLK/RLP sequences in the training dataset, the accuracy of the Phyto-LRR prediction program reached 99%, and for those in the independent test dataset, the accuracy was about 92%, indicating that this program could identify LRR motifs in plant LRR-RLKs/RLPs with high efficiency. Since there are only tens of the LRR- RLK/RLP sequences with crystal structures available online, the diversity of the current independent test dataset is restrained. To further assess the performance of this program, two LRR-RLK sequences were randomly picked from each of the 17 species, and the LRRs of the ECDs were predicted by other two available PSSM based programs, the LRRfinder and the LRRsearch (Tab. S1). The results showed that the Phyto-LRR prediction program could identify LRR motifs in plant LRR-RLK proteins the most efficiently compared with the other tools. When predicting LRR motifs, Phyto-LRR sometimes missed very divergent motifs located at the N terminal and/or the C terminal, such as the SOBIR1 and PGIP in Table 2, therefore, the database obtained was manually checked (Tab. S2), especially for LRRs in the N terminal and/or the C terminal, before it was employed for further analysis. Despite the predicted LRR motif offsets, the database was also integrated with the prediction of the secondary structures, the soluble accessibility, as well as potential canonical N-glycosylation sites [NxS/T (x ≠ P)] (see Methods).

Table 1 The number of LRR-RLK protein sequences in 17 represented land plants in the Phyto-LRR prediction database
Full size table
Fig. 1
figure 1

The flow chart of the Phyto-LRR-prediction program. a The process of training the PSSM matrix. b Flow chart describing the prediction process of the program

Full size image
Table 2 The performance of the Phyto-LRR prediction program. Sequences from the PDB files were used to examine the performance of the LRR prediction program
Full size table

The distribution of LRR-RLKs in different LRR-RLK subgroups of different species

In total, 2999 protein sequences from the Phyto-LRR database containing a signal peptide, LRR(s), TM, and a KD were classified into 18 SGs based on the maximum likelihood (ML) phylogenic analysis of the KD sequences in IQtree (Table 3; Fig. S1, Tab. S3; See Methods). To test the robustness of the ML tree, ten subsets containing about 300 sequences consisting of ~ 10% random sequences from each SG noted in the global phylogenetic tree were selected (Tab. S3) to construct the test ML phylogenic trees. The results showed that the SGs were mainly monophyletic in most test trees except that SG_x, SG_xi, and SG_xii, showing one to three sequence were placed outside the main monophyletic clade in more than five test trees. The distribution features of the LRR-RLK sequences were then examined. According to Fig. 2, SG_iii, SG_xi, and SG_xii had a larger number and percentage of LRR-RLK sequences than other SGs in all 17 species. By contrast, although ancient species, such as PHYPA, MARPL, PHODC AMBTR, and SELML, contained fewer sequences in each SG (Fig. 2a), the distribution pattern of the sequences in each SG were not significantly different from other species in most SGs (Fig. 2b). Interestingly, in the ancient species and the GLYMA, the sequences accounted for a much higher percentage in SG_xi than in other species (Fig. 2b).

Table 3 The number of LRR-RLK protein sequences for phylogenetic analysis and the average number of LRR repeats in the extracellular domain (ECD) of these genes for each subgroup (SG) among 17 species
Full size table
Fig. 2
figure 2

The distribution of potential LRR-RLK sequences from different species in different subgroups (SGs). a The number of LRR-RLK candidates in different SGs from different species. b The percentage of LRR-RLK candidates in different SGs of each species. The global perspective of the sequences in each SG was presented as a heatmap using the R package pheatmap [27]

Full size image

The LRR motif arrangement in different LRR-RLK SGs

Based on the phylogenetic analysis and the Phyto-LRR database, the distribution pattern of the LRRs in the ECDs was determined. According to peaks of each violin plot in Fig. 3a, for most SGs, the probability densities of the LRR numbers in each sequence were high in a small interval with a small percentage of outliers except for SG_x, which held two peaks (almost equal) of the LRR number, concentrated around 4–10 and 18–22, respectively, implying that there were two distinct ECDs in the SG_x and that these two subclades in SG_x should function in different ways. Since, these two subclades intersected with each other in the big tree, further phylogenetic analysis of the KDs of SG_x were conducted. The results turned out that these two subclades in SG_x were clustered separately, therefore, in this work, the SG_x was further divided into SG_x_1* and SG_x_2* (Fig. 3b). The density of the LRR motifs in the ECDs was also calculated, which is described as the number of LRRs per 100 amino acids. As shown in Fig. 3c, SG_i had the lowest LRR density, which was less than 0.6 LRRs per 100 amino acids, implying that only a few LRRs existed in the ECDs of the SG_i members. By contrast, in SG_vii, SG_x_2*, SG_xi, and SG_xii, LRR motifs exceedes 3.3 per 100 residues, suggesting that LRR motifs appeared continuously with few insertion sequences in these SGs. In agreement with Fig. 3a, the two sub-clusters in SG_x showed distinct LRR densities (Fig. 3c).

Fig. 3
figure 3

LRR motif distribution pattern in plant LRR-RLKs. a The number of predicted LRR motifs in different SGs. b SG_x_1* and SG_x_2* were clustered in the refined phylogenetic tree. Refined phylogenetic tree of SG_x was constructed based on the kinase domains (KDs) using the neighbor-joining (NJ) method within MEGA software (version 7.0.26). The bar indicated a mutation rate of 0.10 substitutions per site. Bootstrap values of 1000 replications were shown near the branch. c LRR motifs distribution of the plant LRR-RLK ECDs from different species in different SGs. LRR motifs per 100 amino acids of the ECDs were calculated and shown as a heatmap using the R package pheatmap [27]

Full size image

The features of the residues located on the inner side of the superhelical LRR assemblies

In plants, for sequences with tens of continuous LRRs and few insertion segments, the ectodomain of the LRR-RLKs tended to stack into superhelical shapes. Such superhelical ECDs tend to act as receptors to sense various ligands for signal activation [28,29,30]. The structures of the LRR assemblies are predictable due to the high conservation of the LRR repeats, with the “LxxLxLxxN” forming the inner side of the superhelix, the “xLs/tG” forming the plant-specific second β-sheet on the lateral side, and the remainder forming the backside (Fig. 4a-c). Since proteins tend to bury their hydrophobic patches inside during the folding process, the prediction of the solvent accessibility of the LRR motifs in such LRR stacks may assist in better understanding their important structural elements. Plant LRR-RLKs in SG_vii, SG_x_2*, SG_xi, and SG_xii had fewer sequential insertions (Fig. 3c) and tended to form superhelical structures with more than 20 LRRs. Therefore, sequences of this type were selected (Fig. 3a), and the average solvent accessibility scores of each SG were predicted by ACCpro20 [31] (Fig. 4d). The results showed that the conserved residues on the LRR backbone were more hydrophobic than variable residues, even for the hydrophilic residues, such as the conserved asparagine (9th) and glycine (13th), with “L” sites the most hydrophobic (Fig. 4d). Moreover, variable residues at the 3rd, 7th, 18th, and 20th sites had lower hydrophilicity than other variable residues (Fig. 4d), which might, to some extent, be more important in protein proper folding.

Fig. 4
figure 4

The LRR motif signatures in plant LRR-RLKs SG_vii, SG_x, SG_xi and SG_xii with more than 20 LRR repeats. a The consensus sequences of the LRR motif. b The model of a plant LRR unit. c The model of LRR stacking of continuous LRR repeats. Typical extracellular LRR architectures (based on PDB code 3RGZ) are shown. The LxxLxLxxN motifs were taken as the internal side of the superhelix, the xLsG motifs were taken as the lateral of the superhelix, and the xIPxxLxxLxx motifs were taken as the external side of the superhelix. The motifs are shown in magenta, cyan, and yellow, respectively. d The average solvent accessibility of LRR motifs. The solvent accessibility of the LRR-RLKs extracellular domains was predicted in the SCRACH-1D program [31]. The average solvent accessibility value of residues in LRR motifs predicted by ACCpro20 is shown

Full size image

The evolutional analysis and structural analysis revealed that variable residues located on the inner side of the superhelical stacks were crucial for LRR-RLKs ligand perception [7, 16]. Based on the Phyto-LRR database, the residues could be comprehensively profiled in both sequential and spatial dimensions, i.e. both the sequential conservation of the residues and their special localization at the superhelix could be displayed, which will assist in finding important functional residues of the LRR-RLKs with convincing homolog models. Here, the author profiled the residues located at the inner side of the superhelical stacks in several homolog clusters containing well-studied LRR-RLKs. The Arabidopsis BRI1 (AT4g39400), PEPR1 (At1g73080), FLS2 (At5g46330), HAE (At4g28490), TDR/PXY (At5g61480), PSKR1 (At2g02220), and RGFR1 (At4g26540) protein sequences were used as queries to perform BLASTP search for their own homologous sequences in the LRR-RLK sequences in the global ML tree (Table 3; Fig. S1; Tab. S3). For each group of sequences, protein sequences of the top 300 hits were selected and 10 hits (or all hits if the total number was less than 10) in each genome were chosen (Tab. S9) for phylogenetic analysis. The BRI1, PEPR1, FLS2, HAE, TDR/PXY, PSKR1, and RGFR1 subclades were extracted for further residue analysis (Fig. S2). For each homolog subclade, the logo of the LRRs and the logos of the residues at each site of each LRR were created (Fig. S3), so that the residues conservation at each site could be observed (Fig. 5). In this work, The BRI1-subclade and PSKR1-subclades belonged to SG_x_2*, the FLS2-subclade belonged to SG_xii, and the remainder originated from SG_xi. In each subclade, residue logos at each site of each LRR were created. According to Fig. 5, although the variable residues at the 2nd, 3rd, 5th, 6th, and 7th sites in the LRR backbone were distinct among clades, in close homolog subclades, they were highly conserved in certain positions. Some of the highly conserved residues have been well-documented, such as the RxR motif in AtPEPR1, AtHAE, AtGRFR1, and AtPXY (Fig. 5c-f, motifs denoted in magenta boxes), others remain less known. Since these highly conserved residues appeared at ligand binding areas in their well-studied Arabidopsis homologs (Fig. 5, LRRs highlighted in red bars), these residues might be involved in ligand recognition. Moreover, residues lying on the 5th and 7th sites in each LRR are often exhibited DLS or NLS motifs, and such residues pairs tended to appear in LRRs not involved in ligand binding, indicating that these DLS or NLS motifs might contribute to protein structural integrity.

Fig. 5
figure 5

The sequential characteristics of the variable residues in the inside of the superhelix. The footprints of residues along the inner side of the LRR stacks in seven homolog clades (Fig. S2 and S3) are shown. Red bars indicated LRR areas involved in ligand binding according to LRR-ligand complexes: BRI1 (PDB 4M7E), PSKR1(PDB 4Z61), PEPR1 (PDB 5GR8), HAE (PDB 5IXQ), RGFR (PDB 5Z21), TDR/PXY (PDB 5GIJ), and FLS2 (PDB 4MN8). The conserved RxR motifs in sequences belonging to SG_xi are highlighted in magenta boxes

Full size image

The distribution of the N-glycosylation sites in the LRR-RLK ECDs

Another important feature of plant LRR-RLK ECDs is that they are heavily N-glycosylated. According to the analysis of the Arabidopsis manually annotated proteome GFF file from Swiss-Prot, among the 156 identified LRR-RLKs, more than 80% of sequences harbored an average of more than 5 N-glycosylation sites and 98% of the N-glycosylation sites were annotated to be N-glycosylated (Tab. S4), implying that N-glycosylation is an important modification and could be predicted based on the NxS/T (x ≠ P) sequons. Here, N sites on the NxS/T (x ≠ P) sequons were denoted as N+ for short and their distribution features were illustrated. At first, the number of N+ per 100 amino acids of the ECDs were determined in each SG from different species. As shown in Fig. 6a, no apparent clusters of N+ density differences could be observed among different plant species. The number of N+ was rich in SG_ii, SG_vii_2, SG_viii_2, SG_x_2*, SG_xi, SG_xii and SG_xiv, especially in SG_xiv from MEDTR, GLYMA, POPTR, SOLTU, PHODC and SOLLY. By contrast, in SG_v and SG_vi_2, the N+ density was lower than that in other SGs. The ratio between NxS and NxT was also examined, and it showed that plant LRR-RLKs had a preference for NxS sequons in all SGs except SG_viii_1 and SG_ix (Fig. 6b). N+ sites were mainly localized on LRR repeats in most SGs, especially for SG_vii, SG_x_2*, SG_xi, and SG_xii, whereas they were mainly not localized on LRR repeats in SG_i (Fig. 6c) due to its lower LRR concentration of ECD (Fig. 3c).

Fig. 6
figure 6

The distribution of N+ of the plant LRR-RLKs extracellular domains. a N+ distribution of the plant LRR-RLK extracellular domains from different species in different SGs. The N sites in NxS/T (x ≠ P) are noted as N+ for short. N+ per 100 amino acids of the ECDs was calculated and showed as a heatmap using the R package pheatmap [27]. b The types of N+ in each SG. The number of NxS (x ≠ P) and NxT (x ≠ P) motifs in different SGs was determined. c N+ distribution of the plant LRR-RLKs ECDs internal or external to LRR motifs. N+ locations in the range from the start point of the indicted LRR motifs to the start point of the LRR motifs plus 24 were considered as N-glycosylation sites located in LRRs

Full size image

Plant LRR-RLKs in SG_vii, SG_x_2*, SG_xi, and SG_xii had fewer sequential insertions (Fig. 3c) and tended to form superhelical structures with more than 20 LRRs. Well-studied proteins of this type are mainly proved to be receptors responsible for ligand recognition, which plays important roles in plant development and responses to environmental stresses [16, 18, 32, 33]. Since the structures of these types of LRR-RLKs could be well predictable through sequences (Fig. 4a), the distribution pattern of N+ and N− (N sites not in the NxS/T (x ≠ P)) located on the internal, lateral, and external side of the LRR stacks in these LRR-RLKs were further depicted. Since a great amount of N− located in the highly conserved N sites on the 9th sites of the CS on the inner side of the superhelix, N− at these locations were ruled out. For asparagine residues on the internal and external side of the superhelix, only 40% were N+; in contrast, 70% located on the lateral side were N+ (Fig. 7a and c). A high ratio of over 85% of N+ sites lay on the 5th, 8th, 10th and the 21st variable sites in the plant LRR consensus sequences (Fig. 7c; Fig. 4d, variable residues colored in red), where the average soluble accessibility scores of the − 2, − 1, and + 1 residues next to N+ were very low (Fig. 7b). By contrast, ~ 50% N− located on the 5th, 8th, 10th, and 21st variable sites (Fig. 7f), and the average soluble accessibility scores of the − 2, − 1, and + 1 residues next to N− were higher than those of N+ (Fig. 7b and d). Interestingly, continuous N+ or N− sites could be observed on the internal and the lateral side rather than the external side (Fig. 7c and f). Moreover, in comparison with N−, N+ on the inner side of the superhelix tended to locate at the N or C terminal rather than in the middle, whereas N+ located on the backside preferred the N terminal and the middle (Fig. 7g and h).

Fig. 7
figure 7

The signatures of N+ and N− in SG_vii, SG_x, SG_xi, and SG_xii with more than 20 LRRs. a and d The total number of N+ and N− the internal, lateral, and external areas of the plant LRR-RLK ectodomain. b and e The solvent accessibility of the residues next to N+ and N− on the internal, lateral, and external side of the plant LRR-RLK ectodomain superhelix. The ACCpro20 values were calculated, and the average ACCpro20 values of residues located −5 to + 5 from the N sites are shown. c and f The motif signatures of N+ and N− on the internal, lateral, and the external of the plant LRR-RLK ectodomain superhelix. The signature of residues located − 5 to + 5 from N+ and N− are shown in weblogos, where hydrophilic, neutral and hydrophobic residues are colored blue, green, and black, respectively. g and h The number of N+ and N− at the N terminal, C terminal, or the middle area of the superhelix. LRRs located in the N terminal, middle, and C terminal LRRs were noted. N+ and N− located in the internal side, lateral side, and backside of the N terminal, middle, and C terminal of the superhelix were calculated

Full size image

Discussion

In this study, based on the PSSM algorithm of 16-residue plant-specific LRR-HCS (“LxxLxLxxNxLstGxIP”), a Phyto-LRR prediction program was constructed (Fig. 1). In employment of this LRR-prediction tool, more than 55,000 LRRs were detected from ~ 4000 protein sequences containing LRR(s), TM and KD domain from 17 land plant species (Table 1) were stored in a database for further analysis (http://phytolrr.com/). Sequences containing signal peptides further underwent ML phylogenetic analyses, and 18 SGs were then classified (Fig. S1; Tab. S3). The results revealed that although ancient species contained a lower number of LRR-RLKs (Table 3, Fig. 2a), the distribution pattern of the sequences are similar among species (Fig. 2b). The LRR(s) arrangement pattern (Fig. 3), the residues in the ligand-binding areas (Figs. 4 and 5), and the asparagine resides for N-glycosylation (Figs. 6 and 7) were then analyzed.

The position-specific scoring matrix (PSSM) is derived from a set of aligned sequences, therefore, this perceptron algorithm strongly depends on the training dataset [34]. There are three PSSM based LRR motif prediction programs, LRRfinder, LRRsearch, and the Phyto-LRR program. The LRR-finder program is trained with a non-redundant dataset comprising publically available Toll-like receptors (TLRs) sequences, which are the “typical” LRR class structure [26]. The position-specific scoring matrix (PSSM) was created to represent the 11-residue (LxxLxLxxNxL) LRR highly conserved amino acid positional distributions; the LRRsearch program was trained by the same 11-residue LRR highly conserved sections of 421 NOD-like receptors (NLRs) [25]; and the Phyto-LRR prediction program was trained by the 16-residue [LxxLxLxxNxL(s/t)GxLP] plant-specific LRR highly conserved sections from 17 representative land plants. Due to the preferences of the training datasets, the LRRfinder performed well for Toll-like receptors (TLRs), LRRsearch performed much better in cytoplasmic NOD-like receptors (NLRs) [25, 26], and the Phyto-LRR identified LRR motifs in plant LRR-RLK proteins more efficiently (Tab. S1). In comparison with LRRsearch, the Phyto-LRR could detect plant LRRs with divergent sequences, this should be attributed to that the perceptron of Phyto-LRR was trained with over 4000 plant LRR highly conserved motifs (Tab. S7) and was adjusted with the Laplace smoothing algorithm. The Phyto-LRR prediction module is available by local users at http://github.com/phytolrr so that a bunch of sequences could be detected at one time to better facilitate researches. Moreover, the training dataset function was open for users to trained the program to adjust to their own LRR-containing protein families, therefore, they could use the module to predict and analyze their interested LRR-containing protein families with high efficiency.

Plant LRR-RLKs are important membrane-localized receptors sensing various ligands to regulate plant developmental processes. The classification of LRR-RLKs is usually based on the alignment of the KDs, because the alignment of the ECDs is too ambiguous for phylogenetic analysis. In this work, ~ 3000 LRR-RLK sequences from 17 represented land plant species were classified into 18 SGs based on the alignment of their KDs. The SGs in the ML tree agree with most of the reported classifications [2, 6, 7, 12, 25, 26], and the robustness of the tree is supported by 10 test trees (Tab. S3). Similar to previously reported findings [10], most of the SGs had a similar pattern of LRR distribution in the ECDs, except SG_x, which was apparently divided into two distinct LRR distribution patterns (Fig. 3a). These two clusters were then named as SG_x_1* and SG_x_2* based on further Neighbor Joining phylogenetic analysis of SG_x (Fig. 3b), which was in well accordance with the phylogenetic branches by Fischer et al. [7]. The distinct LRR arrangement of SG_x_1* and SG_x_2* indicated that they might be involved in distinct mechanisms when activating signal transductions [16] and support the previous hypothesis that fusion of the kinase domain with different extracellular structures led to the current land plant RLK gene family [2, 12]. Interestingly, although the SG_x were divided into two subclades based on Neighbor Joining, it was monophyletic according to the ML tree and the two subclades SG_x_1* and SG_x_2* were intersected in the ML topology (Fig. S1), therefore further phylogenetic analysis of SG_x would unveil more evolutional significance of this SG. Comparison of paralogous genes revealed many LRR-RLK SGs have a ω > 1 (dN/dS ratio, the non-synonymous/synonymous substitution rates) [2, 7], indicating a net acceleration of protein evolution [35]; these genes are mainly distributed in the ECDs [2, 36, 37], especially for those bolded and underlined residues in the LxxLxLxxN segment [7], located on the inner side of the superhelices assemblies [16, 18]. Several well-documented LRR-RLKs, such as BRI1 [20, 38], PSKR [39], RGFR1 [40], HAE [30], TDR/PXY [41], FLS2 [42], and PEPR1 [43], have been crystallized in forms of receptor-ligand binding. When analyzing the residues at each LRR in their homologs, it could be observed that the seemingly variable residues are distinct among homolog subclades, but to some extent conserved within each clade (Fig. 5), especially for residues located the ligand-binding domain (red bars in Fig. 5). Some of these highly conserved residues in Fig. 5 have been reported to be essential for ligand recognition in the Arabidopsis homologs, such as the RxR motif in the SG_xi homolog clades [16], S437 and T342 in FLS2 clades [42], and G186, Y188, G210, Y234, D255, D303, S305, W353, D375, and S377 in PXY [41]. Others, such as D414 in FLS2 and D273 in EPER1 clades, are to some extent varied, although the residue at these sites in the Arabidopsis homologs interacted with ligands [42], which might result from the functional mechanism variation among homologs in different plant species. The roles of those that are highly conserved in homologs and are located in the receptor-ligand interaction regions remain obscure and require further investigation. Therefore, this type of LRR prediction together with the proper modeling of the 3D structure will favor the study of plant LRR-RLKs. For example, residues lying on the 7th sites in each LRR often showed Ser residues (Fig. 5), and continuous serine residues tend to appear in LRRs not involved in ligand binding. Intriguingly, three weak BRI1 mutations [bri1–9, bri1–706 (S253F), and bri1–235 (S156F)] were identified and have been proved to be structurally imperfect but functionally competent mutants [44,45,46]. Most recently, the author (2020) found that the serine residues at the 7th site in AtBRI1 played important roles in protein proper folding in ER, while those non-serine residues at the ligand-binding region along the 7th sites were crucial for AtBRI1 function [47]. Moreover, residues lying on the 5th and 7th sites in each LRR often showed DLS/NLS motifs (Fig. 5), and continuous DLS/NLS motifs tend to appear in LRRs not involved in ligand binding. According to the BRI1 PDB files (3RGZ), there were polar contacts between the D and S residues in each LRR. Moreover, the NLS would supply N-glycosylation sites, which are beneficial for protein folding. These findings indicated that the DLS or NLS motifs should contribute to the protein structural integrity, yet more need to be done to reveal the underlying mechanisms. Furthermore, the island domain, an insertion section between LRRs is believed to be of great functional importance in LRR-RLK ligand binding [16, 32, 33], therefore Phyto-LRR’s efficient detection of the island domain will also favor the LRR-RLK functional study.

In Arabidopsis, based on the NxS/T (x ≠ P) motif, approximately ~ 1200 out of 4000 secretory glycoproteins contain more than five canonical N-glycosylation sites [48]. The N-glycoproteomic studies from representative eukaryotes showed that approximately 45% of the identified proteins have more than one identified N-glycosylation sites [49, 50]. In total, 82% of Arabidopsis LRR-RLKs had more than five canonical N-glycosylation sites (NxS/T (x ≠ P) sequons). LRR-RLKs with multiple N-glycosylation modifications have been confirmed by crystalizing [20, 30, 38, 39, 41, 42] or proteome analysis [49]; however, due to the limits of the technologies, the results are still not comprehensive [51]. Since most of the NxS/T (x ≠ P) sequons tended to be modified with N-glycans according to the manually checked Arabidopsis proteome GFF file from Swiss-Prot (Tab. S4), the analysis of the potential N-sites might help with the understanding of the function of this modification. These heavy N-glycosylation modifications are crucial for LRR protein structure and biological functions [22, 23, 52, 53]. In this work, N sites in the NxS/T (x ≠ P) sequons (N+ in this work) tended to localized at the 5th, 8th, 10th, and 21st variable sites in the plant LRR consensus sequences (Fig. 7c; Fig. 4d, variable residues colored in red). Moreover, the average soluble accessibility pattern of resides − 5 to + 5 next to the N sites was similar to that of N+ and N−, although the − 2, − 1, and + 1 residues were more hydrophobic for N+ than N−, indicating that N-glycosylation modification in LRR-RLKs tended to cover the local hydrophobic patches [55], and the deletion of one, might, to some extent, not cause dramatic destruction of the receptor structures [19, 53, 54] (Fig. 7b and e). Therefore, for individual LRR-RLKs/RLPs, the contributions of the N-glycans at different sites might not be identical: some sites are seemingly erasable without conferring any impacts on protein folding and bioactivity, and some could play critical roles for protein abundance and ligand recognition independently [19, 21]. More informative mechanisms underlying site-specificity of the N-glycosylation modifications should be interpreted based on the analysis of the crystal structures or the homolog modelling structures [55].

Conclusion

Based on the “Phyto-LRR prediction”, an effective program for predicting the LRR segments in plant LRR-RLKs, the plant LRR-RLKs ECDs were comprehensively analyzed, revealing important characteristics of the residues in LRR motifs. This LRR prediction program and the ECD database will benefit the functional research of plant LRR-RLKs.

Methods

Studied genomes

In total, genomes from 17 representative land plants were analyzed (Table 1), including angiosperms (4 monocots [sub] species and 10 dicots species), liverwort, moss and spikemoss: Phoenix dactylifera, Oryza sativa ssp. japonica, Oryza sativa ssp. indica, Brachypodium distachyon, Zea mays, Solanum tuberosum, Solanum lycopersicum, Arabidopsis thaliana, Arabidopsis lyrata, Brassica rapa, Populus trichocarpa, Glycine max, Medicago truncatula, Amborella trichopoda, Marchantia polomorpha, Physcomitrella patens, and Selaginella moellendorffii. Throughout this article, the species were referred using five-digit identifiers as shown in Table 1. Details on genome versions can be found in Tab. S5.

The extraction of the potential LRR-RLK protein sequences

Protein sequences containing both intact (i.e. non-degenerated) LRR(s) and a KD were extracted by running the hmmsearch (HMMER 3.2.1) program as described previously [56]. The TMs were predicted using TMHMM http://www.cbs.dtu.dk/services/TMHMM/ websites hosted at the Center for Biological Sequence Analysis, Technical University of Denmark [57]. Protein sequences containing LRRs, a TM and a KD were obtained and those encoded with the same gene ID were further filtered by picking up the longest sequences and sequences with unexpected characters were also removed. The ECDs and KDs of the protein sequences were then extracted, respectively, according to the ClustalW alignment in Mega 5.0 with default argument settings [58]. The obtained ECD sequences and the KD sequences were then checked by similar hmmsearch program for LRR and KD search, respectively (E value cut-off < 1). The non-redundant sequences (3987 sequences), which had LRR(s) in the N termini side, TM and a KD in the C termini, were taken as LRR-RLKs and were used for LRR motif prediction by Phyto-LRR prediction program and stored in the Phyto-LRR database. 2999 out of 3987 sequences were obtained after filtering with SignalP 5.0 [57] at http://www.cbs.dtu.dk/services/SignalP/, which were taken for further phylogenetic analysis and sequential assessment in the current article.

The Phyto-LRR prediction program

The Phyto-LRR prediction program was constructed based on the PSSM algorithm as described previously [25] with some optimizations (Fig. 1). To avoid the zero-probability problem, the Laplace smoothing algorithm was used when the basic position frequency matrix (PFM) convert to the position probability matrix (PPM). The overlapping LRR motifs were discarded by selecting the non-overlapping LRR group with the highest score. There were two steps of training to create the PSSM weight matrix. Firstly, a total of 98 Arabidopsis LRR-RLK protein sequences containing more than 4 LRRs were chosen. The LRR motifs were extracted according to the annotation in UniProt at https://www.uniprot.org/ and the NCBI at https://www.ncbi.nlm.nih.gov with manual verification. ClustalW alignment was carried out in MEGA 5.0 to get a snapshot of the highly conserved sequence segments [58]. A total of 1467 16-residue segments, “LxxLxLxxNxLs/tGxIP”, were used for the first-round training (Tab. S6). Secondly, 10% of the 2999 sequences were randomly selected and 8 Arabidopsis LRR-LRKs with crystal structures were also added. The LRR motifs were then predicted from these sequences by the Phyto-LRR prediction program with manual verification. The 16-residue segments were used for the second-round training to adjust the PSSM weight matrix (Tab. S7).

Construction of the Phyto-LRR database

The plant LRR-RLK database (Phyto-LRR database) was created using MySQL. A total of 3987 non-redundant ECD sequences of plant LRR proteins from 17 plant species (Table 1) were inserted into the database and each entry was updated with additional information such as the gene ID and the ECD length. The LRRs were identified by the Phyto-LRR prediction program, and the results were manually checked before integrated into the database. The deleted LRR motifs were shown in the database (http://phytolrr.com), and those manually added into the database were listed in Tab. S2A. The LRR motif candidates were listed in Tab. S2B. The database was also integrated with the prediction of the sequence second structures using the SSpro in the SCRATCH-1D suite [31]. Potential canonical N-glycosylation sites [Asn-x-Ser/Thr (x ≠ Pro)] were also included in the database.

Sequences clustering, phylogeny, and analyses

In the present article, 2999 sequences containing signal peptide, LRRs, TM and KD were used for further phylogenetic, sequential and N-glycosylation motifs analysis. The SGs were classified using the KDs by global phylogenetic analysis (Table. 3; Fig. S1; Tab. S3). The KD sequences were aligned and cleaned with MAFFT (v7.245) [59] and trimAl [60] as described by Fischer et al. (2016) [8]. A maximum likelihood (ML) phylogenic tree was inferred using IQtree with autodetected models (JTT + F + G4 model) [61, 62]. Commands to generate the ML tree are available at http://github.com/phytolrr. SGs were defined manually using the Arabidopsis genes as a reference [2, 9]. The monophyletic type of each SG was further confirmed by ten ML trees in IQtrees from ten subsets of about 300 sequences, which were created by picking approximately 10% sequences randomly from each SG noted in the global phylogenetic tree (Tab. S3). The alignments of the KDs in SG_x were performed using ClustalW and MUSCLE programs in Mega 7 [63]. The phylogenetic tree was constructed using the Neighbor Joining (NJ) method in Mega 7. A total of 1000 bootstrap replications were performed to test the robustness of internal branches. The number of LRR motifs of each sequence and the distribution of the asparagine (N) sites in the potential N-glycosylation sties (N+) were then determined based on the Phyto-LRR database. The soluble accessibility was predicted by ACCpro20 programs in the SCRATCH-1D suite [31] and the average ACC20 values of the residues around N+ were also examined (Tab. S8). The data was then analyzed and showed using ggplot2 package in R [64]. Codes to generate Tab. S8 are available at http://github.com/phytolrr.

The residue analysis at the inner side of the LRR-RLK ECDs

The Arabidopsis BRI1 (AT4g39400), PEPR1 (At1g73080), FLS2 (At5g46330), HAE (At4g28490), TDR/PXY (At5g61480), PSKR1 (At2g02220), and RGFR1 (At4g26540) protein sequences were used as queries to perform BLASTP search for their own homologous sequences in these 2999 LRR-RLK sequences (Table 3; Fig. S1; Tab. S3). For each group of sequences, protein sequences of the top 300 hits were selected and ten hits (or all of the hits if the total hits were less than 10) in each species were then chosen (Tab. S9). The KDs were aligned using MAFFT (v7.245) [59] with auto settings. The alignments were cleaned using TrimAl [60] with settings to only remove sites with more than 80% of gaps and the ML tree was inferred in IQtree with auto-detected models [61, 62]. Sequences from the BRI1, PEPR1, FLS2, HAE, TDR/PXY, PSKR1, and RGFR1 subclades were extracted for further residue analysis (Fig. S2). For each clade, full sequences of the homologs were aligned, and the LRR motifs of each sequence were denoted using the LRR offsets of the query sequences in the Phyto-LRR database as indicators. The LRRs of the homologs denoted in this way were highly identical with those in the Phyto-LRR dataset (> 95%), therefore the aligned LRRs were then slightly manually modified with the LRRs denoted in the database. The residues of each LRR segment located on the superhelical inner side (the LxxLxLxxN segment) were extracted and the residue logos of each site on each LRR among the homolog sequences (Fig. S3) [47] were showed in weblogo [65]. Codes are available at http://github.com/phytolrr.

Availability of data and materials

The “Phyto-LRR prediction” program can be used both online and offline. To predict LRR motifs online, please visit https://phytolrr.com/findlrr. The program is also provided as a PyPI module which could be installed by the command “pip install predict-phytolrr”. All source code can be found at “https://github.com/phytolrr/phytolrr“and “https://github.com/phytolrr/predict-phytolrr“. The database and generation command can be found at “https://github.com/phytolrr/database.

Abbreviations

LRR-RLKs:

Leucine-rich-repeat receptor-like kinases

ECDs:

Extracellular domains

N-glycosylation:

Asparagine-linked glycosylation

TM:

Trans-membrane domain

KD:

Kinase domain

SGs:

Subgroups

CS:

Consensus sequences

HCS:

Highly conserved segment

PSSM:

Position-specific scoring matrix algorithm

References

  1. Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, Layeghifard M, Neuhold J, Lehner A, Kong JX, Grunwald K, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553(7688):342-+.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  2. Shiu SH, Karlowski WM, Pan RS, Tzeng YH, Mayer KFX, Li WH. Comparative analysis of the receptor-like kinase family in Arabidopsis and rice. Plant Cell. 2004;16(5):1220–34.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  3. Lehti-Shiu MD, Zou C, Shiu SH. Origin, diversity, expansion history, and functional evolution of the plant receptor-like kinase/pelle family. Receptor-Like Kinases in Plants. Berlin: Springer; 2012. p. 1–22.

  4. Afzal AJ, Wood AJ, Lightfoot DA. Plant receptor-like serine threonine kinases: roles in signaling and plant defense. Mol Plant-Microbe Interact. 2008;21(5):507–17.

    CAS  PubMed  Article  Google Scholar 

  5. Tang P, Zhang Y, Sun XQ, Tian DC, Yang SH, Ding J. Disease resistance signature of the leucine-rich repeat receptor-like kinase genes in four plant species. Plant Sci. 2010;179(4):399–406.

    CAS  Article  Google Scholar 

  6. Dufayard J-F, Bettembourg M, Fischer I, Droc G, Guiderdoni E, Périn C, Chantret N, Diévart A. New insights on leucine-rich repeats receptor-like kinase orthologous relationships in angiosperms. Front Plant Sci. 2017;8:381.

    PubMed  PubMed Central  Google Scholar 

  7. Fischer I, Dievart A, Droc G, Dufayard JF, Chantret N. Evolutionary dynamics of the leucine-rich repeat receptor-like kinase (LRR-RLK) subfamily in angiosperms. Plant Physiol. 2016;170(3):1595–610.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  8. Wu YZ, Xun QQ, Guo Y, Zhang JH, Cheng KL, Shi T, He K, Hou SW, Gou XP, Li J. Genome-wide expression pattern analyses of the Arabidopsis leucine-rich repeat receptor-like kinases. Mol Plant. 2016;9(2):289–300.

    CAS  PubMed  Article  Google Scholar 

  9. Lehti-Shiu MD, Zou C, Hanada K, Shiu SH. Evolutionary history and stress regulation of plant receptor-like kinase/Pelle genes. Plant Physiol. 2009;150(1):12–26.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  10. Shiu S-H, Bleecker AB. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases. Proc Natl Acad Sci. 2001;98(19):10763–8.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  11. Han GZ. Origin and evolution of the plant immune system. New Phytol. 2019;222(1):70–83.

    PubMed  Article  Google Scholar 

  12. Liu PL, Du L, Huang Y, Gao SM, Yu M. Origin and diversification of leucine-rich repeat receptor-like protein kinase (LRR-RLK) genes in plants. BMC Evol Biol. 2017;17:47.

  13. Matsushima N, Tanaka T, Enkhbayar P, Mikami T, Taga M, Yamada K, Kuroki Y. Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors. BMC Genomics. 2007;8(1):124.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  14. Kajava AV. Structural diversity of leucine-rich repeat proteins. J Mol Biol. 1998;277(3):519–27.

    CAS  PubMed  Article  Google Scholar 

  15. Kobe B, Kajava AV. The leucine-rich repeat as a protein recognition motif. Curr Opin Struct Biol. 2001;11(6):725–32.

    CAS  PubMed  Article  Google Scholar 

  16. Hohmann U, Lau K, Hothorn M. The Structural Basis of Ligand Perception and Signal Activation by Receptor Kinases. Annu Rev Plant Biol. 2017;68:109–37.

    CAS  PubMed  Article  Google Scholar 

  17. Song W, Han ZF, Wang JZ, Lin GZ, Chai JJ. Structural insights into ligand recognition and activation of plant receptor kinases. Curr Opin Struct Biol. 2017;43:18–27.

    CAS  PubMed  Article  Google Scholar 

  18. Hohmann U, Santiago J, Nicolet J, Olsson V, Spiga FM, Hothorn LA, Butenko MA, Hothorn M. Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors. Proc Natl Acad Sci U S A. 2018;115(13):3488–93.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  19. Häweker H, Rips S, Koiwa H, Salomon S, Saijo Y, Chinchilla D, Robatzek S, von Schaewen A. Pattern recognition receptors require N-glycosylation to mediate plant immunity. J Biol Chem. 2010;285(7):4629–36.

    PubMed  Article  CAS  Google Scholar 

  20. She J, Han ZF, Kim TW, Wang JJ, Cheng W, Chang JB, Shi SA, Wang JW, Yang MJ, Wang ZY, et al. Structural insight into brassinosteroid perception by BRI1. Nature. 2011;474(7352):472–U496.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  21. Sun W, Cao Y, Labby KJ, Bittel P, Boller T, Bent AF. Probing the Arabidopsis flagellin receptor: FLS2-FLS2 association and the contributions of specific domains to signaling function. Plant Cell. 2012;24(3):1096–113.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  22. Hong Z, Jin H, Fitchette AC, Xia Y, Monk AM, Faye L, Li JM. Mutations of an alpha 1,6 Mannosyltransferase inhibit endoplasmic reticulum-associated degradation of defective Brassinosteroid receptors in Arabidopsis. Plant Cell. 2009;21(12):3792–802.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  23. Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K, Li JM. Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis. Proc Natl Acad Sci U S A. 2012;109(28):11437–42.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  24. Sonnhammer EL, Eddy SR, Durbin R. Pfam: a comprehensive database of protein domain families based on seed alignments. Proteins. 1997;28(3):405–20.

    CAS  PubMed  Article  Google Scholar 

  25. Bej A, Sahoo BR, Swain B, Basu M, Jayasankar P, Samanta M. LRRsearch: an asynchronous server-based application for the prediction of leucine-rich repeat motifs and an integrative database of NOD-like receptors. Comput Biol Med. 2014;53:164–70.

    PubMed  Article  Google Scholar 

  26. Offord V, Coffey T, Werling D. LRRfinder: a web application for the identification of leucine-rich repeats and an integrative toll-like receptor database. Dev Comp Immunol. 2010;34(10):1035–41.

    CAS  PubMed  Article  Google Scholar 

  27. Kolde R. Pheatmap: pretty heatmaps. R Package Version. 2012;61(926):915.

    Google Scholar 

  28. Diévart A, Clark SE. LRR-containing receptors regulating plant development and defense. Development. 2004;131(2):251–61.

    PubMed  Article  CAS  Google Scholar 

  29. Meng X, Zhou J, Tang J, Li B, de Oliveira MV, Chai J, He P, Shan L. Ligand-induced receptor-like kinase complex regulates floral organ abscission in Arabidopsis. Cell Rep. 2016;14(6):1330–8.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  30. Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M. Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. Elife. 2016;5:e15075.

    PubMed  PubMed Central  Article  Google Scholar 

  31. Magnan CN, Baldi P. SSpro/ACCpro 5: almost perfect prediction of protein secondary structure and relative solvent accessibility using profiles, machine learning and structural similarity. Bioinformatics. 2014;30(18):2592–7.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  32. Xi L, Wu XN, Gilbert M, Schulze WX. Classification and Interactions of LRR Receptors and Co-receptors Within the Arabidopsis Plasma Membrane – An Overview. Front Plant Sci. 2019;10:472.

  33. Chakraborty S, Nguyen B, Wasti SD, Xu G. Plant Leucine-Rich Repeat Receptor Kinase (LRR-RK): Structure, Ligand Perception, and Activation Mechanism. Molecules. 2019;24(17):3081.

  34. Stormo GD, Schneider TD, Gold L, Ehrenfeucht A. Use of the ‘Perceptron’algorithm to distinguish translational initiation sites in E. coli. Nucleic Acids Res. 1982;10(9):2997–3011.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  35. Lynch M, Conery JS. The evolutionary fate and consequences of duplicate genes. Science. 2000;290(5494):1151–5.

    CAS  PubMed  Article  Google Scholar 

  36. Wang GL, Ruan DL, Song WY, Sideris S, Chen LL, Pi LY, Zhang SP, Zhang Z, Fauquet C, Gaut BS, et al. Xa21D encodes a receptor-like molecule with a leucine-rich repeat domain that determines race-specific recognition and is subject to adaptive evolution. Plant Cell. 1998;10(5):765–79.

    CAS  PubMed  PubMed Central  Google Scholar 

  37. Zhang XRS, Choi JH, Heinz J, Chetty CS. Domain-specific positive selection contributes to the evolution of Arabidopsis leucine-rich repeat receptor-like kinase (LRR RLK) genes. J Mol Evol. 2006;63(5):612–21.

    CAS  PubMed  Article  Google Scholar 

  38. Hothorn M, Belkhadir Y, Dreux M, Dabi T, Noel JP, Wilson IA, Chory J. Structural basis of steroid hormone perception by the receptor kinase BRI1. Nature. 2011;474(7352):467–U490.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  39. Wang J, Li H, Han Z, Zhang H, Wang T, Lin G, Chang J, Yang W, Chai J. Allosteric receptor activation by the plant peptide hormone phytosulfokine. Nature. 2015;525(7568):265.

    CAS  PubMed  Article  Google Scholar 

  40. Song W, Liu L, Wang J, Wu Z, Zhang H, Tang J, Lin G, Wang Y, Wen X, Li W. Signature motif-guided identification of receptors for peptide hormones essential for root meristem growth. Cell Res. 2016;26(6):674.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  41. Zhang H, Lin X, Han Z, Qu L-J, Chai J. Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs. Cell Res. 2016;26(5):543.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  42. Sun Y, Li L, Macho AP, Han Z, Hu Z, Zipfel C, Zhou J-M, Chai J. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science. 2013;342(6158):624–8.

    CAS  PubMed  Article  Google Scholar 

  43. Tang J, Han Z, Sun Y, Zhang H, Gong X, Chai J. Structural basis for recognition of an endogenous peptide by the plant receptor kinase PEPR1. Cell Res. 2015;25(1):110.

    CAS  PubMed  Article  Google Scholar 

  44. Sun C, Yan K, Han J-T, Tao L, Lv M-H, Shi T, He Y-X, Wierzba M, Tax FE, Li J. Scanning for new BRI1 mutations via TILLING analysis. Plant Physiol. 2017;174(3):1881–96.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  45. Noguchi T, Fujioka S, Choe S, Takatsuto S, Yoshida S, Yuan H, Feldmann KA, Tax FE. Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids. Plant Physiol. 1999;121(3):743–52.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  46. Li G, Hou Q, Saima S, Ren H, Ali K, Wu G. Less conserved LRRs is functionally important in brassinosteroid receptor BRI1. Front Plant Sci. 2019;10:634.

    PubMed  PubMed Central  Article  Google Scholar 

  47. Chen T, Wang B, Wang F, Niu G, Zhang S, Li J, Hong Z. The evolutionarily conserved serine residues in BRI1 LRR motifs are critical for protein secretion. Front Plant Sci. 2020;11:32.

    PubMed  PubMed Central  Article  Google Scholar 

  48. Rips S, Bentley N, Jeong IS, Welch JL, von Schaewen A, Koiwa H. Multiple N-glycans cooperate in the subcellular targeting and functioning of Arabidopsis KORRIGAN1. Plant Cell. 2014;26(9):3792–808.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  49. Zielinska DF, Gnad F, Schropp K, Wiśniewski JR, Mann M. Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery. Mol Cell. 2012;46(4):542–8.

    CAS  PubMed  Article  Google Scholar 

  50. Song W, Mentink RA, Henquet MG, Cordewener JH, van Dijk AD, Bosch D, America AH, van der Krol AR. N-glycan occupancy of Arabidopsis N-glycoproteins. J Proteome. 2013;93:343–55.

    CAS  Article  Google Scholar 

  51. Tang J, Sun Y, Han Z, Shi W. An illustration of optimal selected glycosidase for N-glycoproteins deglycosylation and crystallization. Int J Biol Macromol. 2019;122:265–71.

    CAS  PubMed  Article  Google Scholar 

  52. Jin H, Hong Z, Su W, Li JM. A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2009;106(32):13612–7.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  53. van der Hoorn RA, Wulff BB, Rivas S, Durrant MC, van der Ploeg A, de Wit PJ, Jones JD. Structure–function analysis of cf-9, a receptor-like protein with extracytoplasmic leucine-rich repeats. Plant Cell. 2005;17(3):1000–15.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  54. Chen T, Zhang H, Niu G, Zhang S, Hong Z. Multiple N-glycans cooperate in balancing misfolded BRI1 secretion and ER retention. Plant Mol Biol. 2020;103:581–96.

  55. Suga A, Nagae M, Yamaguchi Y. Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation. Glycobiology. 2018;28(10):774–85.

    CAS  PubMed  Article  Google Scholar 

  56. Diévart A, Gilbert N, Droc G, Attard A, Gourgues M, Guiderdoni E, Périn C. Leucine-rich repeat receptor kinases are sporadically distributed in eukaryotic genomes. BMC Evol Biol. 2011;11(1):367.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  57. Emanuelsson O, Brunak S, Von Heijne G, Nielsen H. Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc. 2007;2(4):953.

    CAS  PubMed  Article  Google Scholar 

  58. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731–9.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  59. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30(4):772–80.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  60. Capella-Gutiérrez S, Silla-Martínez JM. Gabaldón T: trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics. 2009;25(15):1972–3.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  61. Kalyaanamoorthy S, Minh BQ, Wong TK, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods. 2017;14(6):587.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  62. Hoang DT, Chernomor O, Von Haeseler A, Minh BQ, Vinh LS. UFBoot2: improving the ultrafast bootstrap approximation. Mol Biol Evol. 2017;35(2):518–22.

    PubMed Central  Article  CAS  Google Scholar 

  63. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870–4.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  64. Wickham H. ggplot2: Elegant Graphics for Data Analysis. Switzerland: Springer International Publishing; 2016.

  65. Crooks GE, Hon G, Chandonia J-M, Brenner SE. WebLogo: a sequence logo generator. Genome Res. 2004;14(6):1188–90.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

Not applicable.

Author information

Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 163 Xianlin Ave, Nanjing, 210046, China

    Tianshu Chen

Authors
  1. Tianshu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

T.C designed the project, analyzed the data and wrote the article. The author(s) read and approved the final manuscript.

Corresponding author

Correspondence to Tianshu Chen.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

None of the authors have any competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1: Figure S1.

The maximum-likelihood phylogenic analyses of ~ 3000 LRR-RLK sequences from 17 land plants in Table 1. Figure S2. The phylogenic analyses of LRR-RLK homologs. Figure S3. The procedure to create the residue logo of Fig. 5

Additional file 2: Table S1.

The comparison of LRR predicting programs for predicting plant LRR-RLKs based on PSSM algorithm

Additional file 3: Table S2.

LRR motifs manually added to the Phyto-LRR database

Additional file 4: Table S3.

LRR-RLK members in each SG, refined neighbor-joining (NJ) tree of SG_x, and ten test sets of the phylogenetic tree

Additional file 5: Table S4.

N-glycosylation sites annotated in the Arabidopsis proteome GFF file from Swiss-Prot

Additional file 6: Table S5.

Information on the protein sequence datasets

Additional file 7: Table S6.

Training dataset 1 for Phyto-LRR predict program

Additional file 8: Table S7.

Training dataset 2 for Phyto-LRR predict program

Additional file 9: Table S8.

The distribution pattern of LRR motifs and potential N-glycosylation sites of each LRR-RLK member in different SGs

Additional file 10: Table S9.

Protein sequences blast from 17 plant genomes LRR-RLKs using Arabidopsis protein sequences as a query

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Chen, T. Identification and characterization of the LRR repeats in plant LRR-RLKs. BMC Mol and Cell Biol 22, 9 (2021). https://doi.org/10.1186/s12860-021-00344-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12860-021-00344-y

Keywords

  • Plant LRR-RLKs
  • N-glycosylation
  • Ligand binding
  • LRR motif prediction
  • PSSM

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us