- Research article
- Open Access
The N-glycome of human embryonic stem cells
BMC Cell Biology volume 10, Article number: 42 (2009)
Complex carbohydrate structures, glycans, are essential components of glycoproteins, glycolipids, and proteoglycans. While individual glycan structures including the SSEA and Tra antigens are already used to define undifferentiated human embryonic stem cells (hESC), the whole spectrum of stem cell glycans has remained unknown. We undertook a global study of the asparagine-linked glycoprotein glycans (N-glycans) of hESC and their differentiated progeny using MALDI-TOF mass spectrometric and NMR spectroscopic profiling. Structural analyses were performed by specific glycosidase enzymes and mass spectrometric fragmentation analyses.
The data demonstrated that hESC have a characteristic N-glycome which consists of both a constant part and a variable part that changes during hESC differentiation. hESC-associated N-glycans were downregulated and new structures emerged in the differentiated cells. Previously mouse embryonic stem cells have been associated with complex fucosylation by use of SSEA-1 antibody. In the present study we found that complex fucosylation was the most characteristic glycosylation feature also in undifferentiated hESC. The most abundant complex fucosylated structures were Lex and H type 2 antennae in sialylated complex-type N-glycans.
The N-glycan phenotype of hESC was shown to reflect their differentiation stage. During differentiation, hESC-associated N-glycan features were replaced by differentiated cell-associated structures. The results indicated that hESC differentiation stage can be determined by direct analysis of the N-glycan profile. These results provide the first overview of the N-glycan profile of hESC and form the basis for future strategies to target stem cell glycans.
During the last decade global genomics and proteomics analyses of defined cell populations have revolutionized our understanding of cell biology. Glycomics – the study of global glycan expression profiles – has been predicted to be a next step forward . Glycans, the carbohydrate units of glycoproteins, glycolipids, and proteoglycans, are capable of great structural variation and their specific molecular structures carry vast amounts of biological information . It has been estimated that more than half of all cellular proteins are glycosylated , but little is known of glycan structures in specific cell types. Glycans linked to cell surface proteins and lipids form a dense layer – the glycocalyx – on the extracellular side of the cell surface. The glycocalyx has first-line functions in the communication of the cell and its environment, including both cell-to-cell contacts [2, 4–6] and interactions with extracellular matrix components . In addition, the specific roles of N-glycans involve regulation and control of protein folding [8, 9] and trafficking .
Human embryonic stem cells (hESC)  provide models for the study of human development and toxicology and have therapeutic potential in regenerative medicine . To effectively utilize these cells, novel differentiation stage and lineage specific stem cell markers are required. Since glycans are abundant components of the cell surface, reagents that specifically recognize hESC glycans should be useful tools for the identification, isolation, and manipulation of stem cells. In fact, the monoclonal antibodies currently used to define hESC, including the globo-series glycosphingolipid epitopes SSEA-3 and SSEA-4, and the keratanase-sensitive glycoprotein associated epitopes Tra 1–60 and Tra 1–81, recognize glycan antigens [13–15]. Further, the expansion of undifferentiated hESC and the directed differentiation of hESC to specific progeny lineages in cell culture remain problematic. Understanding how cells interact through the glycocalyx with feeder cells and other components of the culture environment may enable rational design of specific culture systems.
In the present study, a global analysis of the asparagine-linked glycans (N-glycans) of hESC and cells differentiated from them was performed by mass spectrometric profiling of unmodified glycans. We found that hESC have a characteristic and complex protein N-glycosylation profile. The data provide insight into the glycobiology of hESC and can be utilized as a basis for future studies exploring the role of stem cell glycans.
In order to generate mass spectrometric glycan profiles of hESC, embryoid bodies (EB), and further differentiated cells, a matrix-assisted laser desorption-ionization (MALDI-TOF) mass spectrometry based analysis was performed. We focused on the most common type of protein post-translational modifications, N-glycans, which were enzymatically released from cellular glycoproteins. During glycan isolation and purification, the total N-glycan pool was separated by an ion-exchange step into neutral N-glycans and sialylated N-glycans. These two glycan fractions were then analyzed separately by mass spectrometric profiling (Fig. 1 and 2), which yielded a global view of the N-glycan repertoire and allowed comparative analysis of differentiation-associated changes. The present scarce sample amounts did not allow us to purify individual glycan components for structural analyses. However, detailed structural analyses were achieved from the total neutral and acidic N-glycan pools by a combination of proton NMR spectroscopy, specific glycosidase digestions, and MS/MS fragmentation experiments.
MALDI-TOF mass spectrometric analysis has been shown to be accurate in relative quantitation of glycan components within complex glycan mixtures . We and others have found that it is a powerful tool for comparing changes in the glycan composition especially between closely related samples [17–19]. The present MALDI-TOF mass spectrometric methods have been optimized for relative quantitation of non-derivatized glycans and potentially labile glycan residues such as sialic acids are reliably determined. This was further verified during the present work by comparison of non-derivatized and permethylated glycan samples. The relative proportions of the major glycan signals were similar and no sialic acid or fucose loss was detected (data not shown). Due to the large m/z range of observed glycan ions the percentage of each glycan signal might not represent absolute molar percentage of the total cellular N-glycome. The amounts of especially larger glycans may be underestimated. However, we demonstrated with standard molecules that over the m/z range there was a linear response between the amount of added analyte and its relative signal intensity in the recorded glycan profile (see Additional File 1, Supplementary Fig. 1).
Each step in the glycan purification sequence was controlled for reproducibility by mixtures of glycoproteins, synthetic glycans, and glycan mixtures extracted from human cells. We routinely obtained the same mass spectrometric profiles for the standard glycan mixtures after each purification step during the multi-stage purification process  showing that there was no selective loss of glycans during purification. The yield of the N-glycosidase reaction was over 95% with model glycoproteins. The robustness of the present method was further evaluated by subjecting human cell samples to blinded analysis by five different persons. The results were highly comparable showing that the present method reliably reproduced the characteristic glycan profile of each cell type (see Additional File 1, Supplementary Fig. 2). The glycan profile was demonstrated to be reproducible also when different dilutions of the same sample were analyzed, demonstrating that the results were not sensitive to the exact amount of cells in each sample. However, in the present analyses the cell amounts were comparable in the whole sample series.
We analyzed N-glycan profiles of all the biological materials that were in contact with the stem cells and could potentially contaminate the samples with glycoproteins, including hEF and mEF feeder cells, cell culture media, and culture media supplements. The N-glycan profiles revealed that the employed cell harvesting and washing procedures had been efficient and neither the feeder cells nor the culture media glycoproteins had affected the observed stem cell N-glycan profiles to a marked extent. By comparing the characteristic glycan signals in each potential contamination source, we could calculate that N-glycan purity in the samples was at least 93%.
hESC N-glycan profiles
Neutral N-glycans comprised approximately two thirds of the combined neutral and sialylated N-glycan pools of hESC. The relative proportions of the two glycan pools were analyzed by combining corresponding aliquots from the neutral glycan pool and the acidic glycan pool after broad-range sialidase digestion and comparing the combined glycan profile to the separate neutral and acidic glycan profiles (data not shown).
The 40 most abundant neutral N-glycan signals detected in the four hESC lines are presented in Figure 1B (blue columns). The similarity of the profiles with the four hESC lines, which is indicated by the minor variation in the glycan signals, suggests that the four cell lines closely resembled each other. For example, 15 of the 20 most abundant glycan signals were the same in every hESC line. The five most abundant signals (H5N2, H6N2, H7N2, H8N2, and H9N2) comprised 76% of the neutral N-glycan signals and dominated the profile.
All the major N-glycan signals in the acidic N-glycan fraction (Fig. 2B, blue columns) contained sialic acid residues. There was more variation between individual cell lines in the 40 most abundant acidic N-glycans than in the neutral N-glycans. However, the four hESC lines again resembled each other and the five most abundant sialylated N-glycan signals were the same in every cell line: S1H5N4F1, S1H5N4F2, S2H5N4F1, S1H5N4, and S1H6N5F1. The most abundant sialylated glycan signals contained the H5N4 core composition and differed only by variable number of sialic acid (S or G) and deoxyhexose (F) residues. These biantennary-size N-glycans together comprised 61% of the total glycan signal intensity in the acidic glycan fraction.
We detected N-glycans containing N-glycolylneuraminic acid (G) in the hESC samples, for example glycans G1H5N4, G1S1H5N4, and G2H5N4. N-glycolylneuraminic acid has previously been reported in hESC as an antigen transferred from culture media containing animal-derived materials . Accordingly, the serum replacement medium used in the present experiments contained bovine serum glycoproteins. We have recently detected Neu5Gc in N-glycans of hESC and in vitro cultured human mesenchymal stem cells by mass spectrometric N-glycan analysis .
The four hESC lines shared the same overall N-glycan profile and there was only slight cell line specific variation within the profiles. The 30 most common N-glycan signals were the same in all the hESC lines and accounted for circa 85% of the total detected N-glycans. Similarly, EBs derived from each hESC line produced N-glycan patterns with similar characteristics, regardless of the different starting hESC line.
Changes in the N-glycan profile during hESC differentiation
A major goal of the present study was to identify glycan structures that would be specific to either stem cells or differentiated cells, and could therefore serve as differentiation stage markers. In order to determine whether the hESC N-glycome undergoes changes during differentiation, the N-glycan profiles obtained from hESC, EB, and stage 3 differentiated cells were compared (Fig. 1B and 2B). The profiles of the differentiated cell types (EB and stage 3 differentiated cells) were clearly different compared to the profiles of undifferentiated hESC, as indicated by non-overlapping distribution bars in many glycan signals. This suggested that differentiation induced the appearance of new N-glycan types while earlier glycan types disappeared.
Figure 3 presents the observed sample-to-sample variation in relative abundance of four N-glycan signals: S1H5N4F2, S1H5N4F1, and H9N2 (hESC-associated), as well as S1H5N5F1 (differentiated cell-associated). S1H5N4F2 (Fig. 3A) was the major hESC-specific glycan signal and had high abundance in all the four hESC lines, while its relative amount dropped consistently in all samples from both EB and further differentiated cells. In contrast, S1H5N5F1 (Fig. 3B) that was the major differentiation-associated glycan signal, was highly abundant in all EB and further differentiated cell samples, while it was only a minor glycan in all hESC samples. These two glycan signals could serve as markers of either the stem cells or the differentiated cells, respectively. The relative signal intensities of both S1H5N4F1 (Fig. 3C) and H9N2 (Fig. 3D) were on average slightly higher in hESC than in the differentiated cells, but individual differentiated cell samples differed greatly from the average. These glycan signals may therefore not be useful differentiation stage markers as such, but may however indicate trends in glycan biosynthesis.
The major differentiation stage associated N-glycan signal types were also visualized in the sialylated N-glycan profiles (Fig. 2B). There was a significant hESC association in the glycans S1H5N4F2 (Fig. 3A), S1H6N5F2, and S1H5N4F3 as well as other glycan signals that contained at least two deoxyhexose residues (F ≥ 2; marked with red circles under Fig. 2B). Even minor signals with this structural feature were more abundant in hESC than in the differentiated cell types. Sum of the relative signal intensities of all F ≥ 2 N-glycan signals in Fig. 2B was 25% for hESC, 13% for EB, and 11% for stage 3 differentiated cells, indicating that multifucosylation of sialylated N-glycans was gradually decreased during hESC differentiation. Structural analysis of these glycans is presented in the following section.
In contrast, a major group of N-glycan signals which increased during differentiation contained equal amounts of N-acetylhexosamine and hexose residues (N = H) in their monosaccharide composition, e.g. S1H5N5F1 (Fig. 3B). This is consistent with N-glycan structures containing non-reducing terminal N-acetylhexosamine residues. EB and further differentiated cells showed increased amounts of N-glycans expressing such monosaccharide compositions (marked with gray circles under Fig. 2B). Sum of the relative signal intensities of all N = H N-glycan signals in Fig. 2B was 3% for hESC, 14% for EB, and 22% for stage 3 differentiated cells, indicating that this structural feature was drastically increased during hESC differentiation at the EB stage and then further increased in stage 3 differentiated cells. However, these glycan structures were not characterized further in the present study.
Of the three sample types, only hESC were grown in the presence of hEF feeder cells, while both EB and further differentiated cells were grown without hEF. Therefore the observed hESC-specific glycans could have been contaminants derived from hEF. Figure 3 clearly shows that this was not true for the major hESC-associated glycan signal S1H5N4F2 (Fig. 3A) that had very low abundance in both analysed hEF samples. Similar examination of other hESC-associated glycans was consistent with this and we could conclude that any potential hEF-derived contamination was too minor to be detected. The major glycan structures that we identified to be associated with either the stem cells or the differentiated cells were not detected in the potential contamination sources. These structures included the major N-glycan signals with monosaccharide compositions indicating complex fucosylation and terminal HexNAc, for example the glycan signals shown in Fig. 3A–B. These signals were also undetectable in the cell culture media and supplements (data not shown).
The N-glycan profiles of the differentiated cells were also quantitatively different from the undifferentiated hESC profiles. A practical way of quantifying the differences between glycan profiles was to calculate the sum of the signal intensity differences between two samples. According to this method, the EB neutral and sialylated N-glycan profiles had undergone a quantitative change of 14% and 29% from the hESC profiles, respectively. Similarly, the stage 3 differentiated cell neutral and sialylated N-glycan profiles had changed by 15% and 43%. Taking into account that the proportion of neutral to sialylated N-glycans in hESC was approximately 2:1, the total N-glycan profile change was approximately 1/4 during the transition from hESC to stage 3 differentiated cells. The present data thus indicated that the mass spectrometric profile of the hESC N-glycome consisted of two discrete parts regarding propensity to change during hESC differentiation – a constant part and a changing part. As described above, even the minor signals reflected the differentiation stage associated N-glycan structural features. Therefore the mass spectrometric profile in itself was a specific and sensitive marker of hESC differentiation stage.
Statistical analysis of N-glycan profiles
To evaluate statistical differences between hESC and differentiated cell N-glycan profiles, we performed one way ANOVA for each glycan signal (see Additional File 1). The analysis indicated statistical significance for the hESC-associated N-glycan signals, e.g. large high-mannose type N-glycan signals H7N2, H8N2, and H9N2, as well as complex fucosylated N-glycan signals H5N4F2, S1H5N4F2, and S1H5N4F3.
Factor and correlation analyses were employed in order to find common factors which could explain the observed distribution of N-glycan signals between the different cell types. The analyses were performed separately to the neutral N-glycans and acidic N-glycans (data not shown). Observation of the resulting factors indicated structure type related clustering of glycan signal groups in each factor. For example, major contributing signals in Factor N1 (explaining 26% of all variation in the neutral N-glycan fraction) reflected the balance between hESC-associated large high-mannose type N-glycans (H7N2, H8N2); and differentiation-associated low-mannose type N-glycans (H2N2, H3N2, H4N2), whereas Factor N2 (15%) was dominated by differentiated cell types associated glycan types: complex-type N-glycans with N = H type non-reducing terminal HexNAc (H5N5, H5N5F1) and hybrid-type N-glycans (H5N3, H5N3F1, H6N3). Correlation analysis indicated correlation among the large high-mannose type N-glycan signals (H6N2, H7N2, H8N2) and negative correlation with the small high-mannose type N-glycan H5N2.
In acidic glycan factor analysis, Factor A1 (explaining 24% of all variation in the acidic N-glycan fraction) reflected balance between 1) differentiated cell associated hybrid-type N-glycan signals (S1H5N3F1, S1H6N3) and 2) hESC associated complex-type N-glycans (S1H6N5F1, S1H7N6F1, S1H8N7F1) and complex-fucosylated glycans (S1H7N6F3). The major contributing signals in both Factors A2 and A3 (together explaining 23% of all variation in the acidic fraction) were N = H type terminal HexNAc bearing glycans (S1H5N5, S1H5N5F1, S2H5N5F1, S1H5N5F3, S1H6N6F1, S2H6N6F1).
In conclusion, the performed statistical analyses demonstrated that N-glycosylation changes during hESC differentiation were regulated so that the glycan biosynthetic changes were consistently similar from cell line to cell line, which was directly reflected in the level of cellular N-glycan profiles and even distinct glycan signals.
Structural analyses of the major hESC N-glycans
The N-glycan fractions were further analyzed by proton NMR spectroscopy recorded from N-glycans isolated from a larger sample of hESC grown on mEF. The assigned N-glycan structures are included in Figure 4 (for details see Additional File 1, Supplementary Fig. 3 and Supplementary Tables 1 and 2). In the obtained NMR spectrum of the hESC neutral N-glycans, signals consistent with large Man6-Man9 high-mannose type N-glycans were detected. No evidence of other structures was found since the high-mannose type N-glycan structures could explain all signals in the spectrum. In similar analysis of the sialylated N-glycan fraction, all the detected signals were consistent with biantennary complex-type N-glycans with type 2 N-acetyllactosamine (LacNAc) antennae, α2,6- and α2,3-linked sialic acids, and α1,6-linked N-glycan core fucose modifications. No signals corresponding to type 1 LacNAc antennae or other fucose linkages were detected. It was determined by integration of indicator signals that α2,6-linked sialic acids were more abundant than α2,3-linked sialic acids.
In order to validate glycan structure assignments made based on the mass spectrometric and NMR spectroscopic profiling analyses, we performed enzymatic degradation experiments with subsequent mass spectrometric detection (Fig. 4). Comparison of the original and digested N-glycan profiles allowed estimation of the relative proportions of non-reducing terminal monosaccharide residues of neutral N-glycans characteristic of hESCs (Fig. 4A). α-mannose residues were the most common terminal residues in the neutral glycan fraction, occurring in high-mannose type, low-mannose type, and hybrid-type N-glycan signals. β1,4-linked galactose residues occurred in hybrid-type and complex-type N-glycans, whereas terminal β1,3-linked galactose residues were not detected. Non-reducing terminal β-N-acetylglucosamine, α1,2-fucose, and α1,3- or α1,4-linked fucose residues were also present in minor glycan components (data not shown), but not in the major N-glycan signals presented in Figure 4.
The abundances of the major glycan signals in hESC and EB are indicated in Figure 4 and show distinct features in stem cells and differentiated cells. Most specifically, high-mannose type N-glycans were associated with hESC, while low-mannose type and hybrid-type N-glycans were more abundant in EB. These differences suggest that regulation of N-glycan biosynthetic pathways is changed during hESC differentiation. We are currently studying the potential regulatory mechanisms leading to these N-glycan profile differences (TJ et al., manuscript in preparation). The observation of abundant low-mannose type N-glycans is interesting. We observed comparable amounts of fucosylated and non-fucosylated low-mannose type N-glycans in both hESCs and EBs, while analyses of the culture media revealed that they contained only non-fucosylated N-glycans. This indicates that these structures were produced by the studied cells and they did not originate from culture medium glycoproteins.
Analysis of complex fucosylation in N-glycans of hESC
As noted above, there was a significant hESC association in N-glycans containing at least two deoxyhexose residues (F ≥ 2; see Fig. 2B). In contrast, glycan signals such as S2H5N4 that contained no deoxyhexose (F = 0) were increased in the differentiated cell types. This suggested that sialylated N-glycans in undifferentiated hESC were subject to more fucosylation than in the differentiated cell types. The most common fucosylation type in human N-glycans is α1,6-fucosylation of the N-glycan core structure  and this was also the major type of fucosylation detected in the present NMR profiling. In human N-glycans containing more than one fucose residue there should be other fucose linkages in addition to the α1,6-linkage [22, 23], indicating complex fucosylation. The F ≥ 2 structural feature decreased as the cells differentiated, showing that complex fucosylation was characteristic of undifferentiated hESC.
Exoglycosidase analysis of the sialylated N-glycan fractions was performed with specific α1,2- and α1,3/4-fucosidase enzymes to characterize the major hESC-specific N-glycan signals with complex fucosylation (Fig. 5A). Based on the sensitivity of the signal S1H5N4F2 towards the employed fucosidase enzymes, it was shown to include a mixture of isomeric N-glycan structures. Nearly half of the structures within S1H5N4F2 carried one terminal α1,2-linked fucose residue, while the other half carried one terminal α1,3- or α1,4-linked fucose residue. α1,3/4-fucosidase had a larger effect after desialylation (data not shown), indicating that in minor structures there was a fucose residue located subterminal to sialic acid. The majority of the structures contained exactly one fucose residue that was not susceptible to the employed fucosidase treatments, indicating fucosylation in the N-glycan core sequence. This was consistent with the NMR results showing that N-glycan core α1,6-fucosylation was the most abundant fucose linkage in hESC N-glycans. All detected desialylated and defucosylated N-glycans of hESC were sensitive to β1,4-galactosidase but not to β1,3-galactosidase digestion, indicating that the major N-glycan antennae were type 2 LacNAc. Taken together, the results suggested that the fucosylated antennae were either α1,2-fucosylated H type 2 i.e. Fucα1-2Galβ1-4GlcNAc or α1,3-fucosylated Lex i.e. Galβ1-4(Fucα1–3)GlcNAc. Due to limited abundance of the minor structure with fucose residue subterminal to sialic acid, we were not able to sequence it, although a candidate structure is sLex i.e. Neu5Acα2-3Galβ1–4(Fucα1-3)GlcNAc.
MS/MS fragmentation analysis was applied to the most abundant hESC N-glycans with differential fucosylation, S1H5N4F2 (Fig. 5B) and S1H5N4F1 (Fig. 5C). The fragmentation patterns supported the exoglycosidase digestion and proton NMR profiling results and were consistent with sialylated biantennary complex-type N-glycans with predominant core fucosylation. Fucosylated antennae derived from S1H5N4F2 were represented by a single fragment ion at m/z 660.3 that fits to both H type 2 and Lex terminal structures (Fig. 5B), while fragment ions corresponding to sLex were not detected.
Previously the gene expression profiles of the FES hESC lines have been determined  and we extracted the information of the expressed fucosyltransferases from this data (Fig. 5D). The hESCs expressed fucosyltransferase genes FUT1, FUT2, FUT4, and FUT8. Of these genes, FUT1 and FUT4 were overexpressed in hESC when compared to EB. As shown in Figure 5D, in N-glycans expressing type 2 LacNAc antennae (Galβ1-4GlcNAc) the functional expression of the corresponding glycosyltransferase enzymes may produce the following fucosylated epitopes: H type 2 (FUT1), Lex and sLex (FUT4), Ley (combined action of FUT1 and FUT4), as well as N-glycan core α1,6-fucosylation (FUT8). Structures corresponding to Ley (difucosylated LacNAc) were not observed in the present study. Taken together, the evidence indicated that the major fucosylated epitopes in hESC N-glycans were H type 2 and Lex, and additional minor sialylated and fucosylated structures were most likely sLex. However, based on the present experiments it could not be excluded that minor Ley or type 1 structures could be present in hESC N-glycans.
The identified hESC glycans can be targeted at the cell surface
From a practical perspective stem cell research would be best served by reagents that recognize cell-type specific target structures on cell surface. To investigate whether individual glycan structures we had identified would be accessible to reagents targeting them at the cell surface, we performed lectin labeling of three candidate structure types. Lectins are proteins that recognize glycans with specificity to certain glycan structures. Previous studies have extensively described lectin labeling of hESC [25–27] and we have also initiated a study of lectin and glycan antibody ligands on hESC surfaces (MM et al., manuscript in preparation). In the present study, hESC colonies grown on mouse feeder cell layers were labeled in vitro by fluorescein-labelled lectins (Fig. 6). The hESC cell surfaces were clearly labeled by Maackia amurensis agglutinin (MAA) that recognizes structures containing α2,3-linked sialic acids, preferably α2,3-sialylated LacNAc, indicating that such sialylated glycans were abundant on the hESC cell surface (Fig. 6A). In contrast, the cell surfaces were not labelled by Pisum sativum agglutinin (PSA) that recognizes α-mannosylated glycans and potentially also core fucosylated N-glycans (Fig. 6B). However, PSA labelled the cells after permeabilization (data not shown). Finally, Ulex europaeus agglutinin I (UEA-I) that recognizes fucosylated structures, especially H type 2, stained hESC surfaces (Fig. 6C). The specificity of the lectin bindings was validated by inhibition with specific glycan inhibitors as described in the Methods section. Interestingly, the mouse fibroblast cells showed complementary staining patterns compared to hESC, suggesting that these lectin reagents efficiently discriminated between hESC and feeder cells. Figure 6D shows the FACS results with UEA-I further demonstrating that hESC were highly positive for complex fucosylated glycans including H type 2 terminal fucosylated sequences. Consistent with the lectin labeling results, the present structural analyses also demonstrated that both complex fucosylated structures including H type 2 and α2,3-sialylated LacNAc were abundant terminal structures in hESC N-glycans. The results further suggested that the identified glycan structures could be utilized to select reagents specifically targeting undifferentiated hESC, while not binding to other cell types. Glycans expressed on the hESC surface would be good targets for recognition by specific antibodies, which is subject of ongoing research in our laboratories.
In the present study, mass spectrometric and NMR spectroscopic analysis methods were applied in the first structural analysis of hESC N-glycan profiles. Previously, the glycosylation of hESC has been studied with lectins and antibodies [25–27], and a preliminary report has been published on mass spectrometric profiling of mouse embryonic stem cell (mESC) N-glycans . The objective in the present study was to provide a global view on the N-glycan profile, or a "fingerprint" of hESC N-glycosylation, to structurally characterize the most abundant N-glycan structures of hESC, and to compare hESC N-glycosylation with differentiated cells. The hESC N-glycome was found to be characteristic to the cell type and different from either differentiated human cells or mESCs. The data provided information regarding the most characteristic features of hESC N-glycosylation and demonstrated that a dramatic N-glycan profile change takes place during hESC differentiation.
Over one hundred N-glycan signals were detected from each cell type. However, it is important to realize that many of the mass spectrometric signals in the present analyses include multiple isomeric structures and the one hundred most abundant signals therefore represent a larger amount of different glycans. The major N-glycans observed in hESC covered all the major N-glycan classes, namely oligomannose-type, hybrid-type, and complex-type N-glycans , and were decorated with sialylated and fucosylated antennae with equal complexity in all differentiation stages. This directly demonstrated that stem cell N-glycosylation was already as sophisticated as in the differentiated cells.
We found that the hESC N-glycan profile contained both a constant part and a variable part. The variable part was a sensitive indicator of the differentiation commitment. The major glycan types in the constant part were high-mannose type and biantennary complex-type N-glycans. The most characteristic feature of the variable part of the hESC N-glycome was complex fucosylation. In fact, it was found that 26% of the acidic N-glycan signals detected in hESC were multifucosylated. On the other hand, structurally different glycan structures were favoured by the differentiated cell types. About 1/4 of the total N-glycan profile of hESC changed during their differentiation. This demonstrated that during differentiation hESC substantially changed the appearance of their glycocalyx. New N-glycan features emerged in EB and further differentiated cells. These features included additional N-acetylhexosamine residues, potentially leading to completely new glycan epitopes presented on the differentiated cell surface. Such drastic changes in the N-glycome may profoundly alter both cell-cell interactions and the cells' responses to exogenous signals.
Glycans perform their functions in cells by acting as ligands for specific glycan receptors [30–32], functioning as structural elements of the cell , and modulating the activity of their carrier proteins and lipids . More than half of all proteins in a human cell are glycosylated . Consequently, a global change in protein-linked glycan biosynthesis can simultaneously modulate the properties of multiple proteins. It is likely that the large changes in N-glycans during hESC differentiation have major influences on a number of cellular signaling cascades and affect in profound fashion biological processes within the cells.
The major hESC-specific N-glycosylation feature we identified was complex fucosylation. Fucosylation is known to be important in cell adhesion and signalling events [22, 23] as well as being essential for embryonic development . Knock-out of the N-glycan core α1,6-fucosyltransferase gene FUT8 leads to postnatal lethality in mice , and mice completely deficient in fucosylated glycan biosynthesis do not survive past early embryonic development . Fucosylated glycans such as the SSEA-1 antigen, a special form of Lex [35–37], have previously been associated with both mESC and human embryonic carcinoma cells . However, SSEA-1 is not expressed by hESC, which has previously been interpreted such that hESC would not express Lex. A recent report has suggested that mESC proliferation and differentiation can be influenced via specific recognition of fucosylated and sialylated glycoconjugates in a mESC line transfected with L1 receptor .
The published gene expression profiles for the same hESC lines as studied here  have demonstrated that four human fucosyltransferase genes, FUT1, FUT2, FUT4, and FUT8 are expressed in hESC (Fig. 5D), and that FUT1 and FUT4 are overexpressed in hESC when compared to EB (TJ et al., manuscript in preparation). FUT8 encodes the N-glycan core α1,6-fucosyltransferase whose product was identified as the major fucosylated epitope in hESC N-glycans by the NMR analysis. The hESC-specific expression of FUT1 and FUT4, encoding for α1,2-fucosyltransferase and α1,3-fucosyltransferase enzymes, respectively , correlates with our findings of simple fucosylation in EB and complex fucosylation in hESC. The hESC-expressed enzyme product of FUT2 (Secretor) may primarily be linked to other glycan classes such as O-glycans or glycolipids based on its preference for type 1, 3, and 4 chains not detected in N-glycans (MM et al., manuscript in preparation). Interestingly, the FUT4-encoded enzyme is capable of synthesizing both Lex, sLex, and SSEA-1, although the capability to synthesize sLex may be low [39, 40]. We detected N-glycan antenna structures consistent with Lex in hESC N-glycans. Consistent with this, Lex has been reported to be present in mESC N-glycans . N-glycan signals potentially corresponding to sLex were detected in very low amounts in hESC, which is consistent with the reported specificity of the FUT4-encoded enzyme [39, 40]. Our finding of H type 2 structures in hESC N-glycans is a novel feature that differentiates hESC from mESC. However, Wearne et al.  have already reported α1,2-fucosylation in hESC by utilizing UEA-I lectin staining. Significantly, product of the hESC-overexpressed fucosyltransferase FUT1 (H enzyme) is mainly responsible for H type 2 antigen biosynthesis (Fig. 5D). In conclusion, although hESC do not express the specific fucosylated antigen recognized by the SSEA-1 antibody, they share with mESC the characteristic features of complex fucosylation and expression of the Lex antigen. The functions of these major fucosylation modifications in hESC remain to be elucidated in future studies. The present results suggest that the SSEA-1 antibody does not recognize Lex when when presented on a biantennary N-glycan antenna.
Human embryonic stem cell lines have previously been demonstrated to have a common genetic stem cell signature that can be identified using gene expression profiling techniques [41–44]. Such signatures have been proposed to be useful in hESC characterization. In the present report we provide the first glycan profile signatures for hESC. The profile of the expressed N-glycans might be a useful tool for analyzing and classifying the differentiation stage in association with gene and protein expression analyses. In the present work we demonstrated that multiple mass spectrometric glycan signals correlated with the differentiation stage of hESC (Fig. 3). The present results suggest that N-glycan profiling could be developed into a tool for monitoring hESC differentiation status. Glycan profiling might be more sensitive than the use of any single cell surface marker and especially useful for the quality control of hESC-based cell products . However, further analysis of hESC glycans may also lead to establishing new glycan structures as stem cell markers in addition to the commonly used SSEA and Tra glycan structures.
The present lectin staining experiments demonstrated that specific glycan molecules were abundant on the cell surface of hESC. The cell surface presentation of glycans makes them excellent targets for development of cell type specific recognition reagents. It seems plausible that knowledge of the changing surface glycan epitopes may be utilized as a basis in developing reagents and culture systems that would allow improved identification, selection, manipulation, and culture of hESC and their progeny. The present data allow rational selection and evaluation of glycan-specific antibodies based on knowledge of hESC glycan structures.
Venable et al.  and Wearne et al. [26, 27] have extensively characterized hESC and EB reactivity for different lectins. Their results with MAA and UEA-I lectins confirm the present results about the expression of cell type-specific glycan epitopes on undifferentiated hESC surface. These previous studies may also provide cues for differentiation-associated N-glycan changes that were not structurally characterized in the present study. Venable et al.  described that N-glycans modified by bisecting GlcNAc residues as detected by Phaseolus vulgaris erythroagglutinin (PHA-E) were enriched in cells that had low or absent SSEA-4 staining, potentially indicating that such N-glycan structures were early signs of hESC differentiation. Wearne et al.  also found PHA-E ligands on differentiated hESC. It could be hypothesized that part of the terminal N-acetylhexosamine carrying N-glycans (e.g. N-glycans with N = H structural feature, see Fig. 2B) found in the present study to be associated with differentiation cells, could be modified by bisecting GlcNAc. Although specific N-glycan structural information is hard to extract from lectin binding profiles, the data of these earlier reports support our findings of abundant terminal α-mannose and LacNAc residues, both α2,3- and α2,6-sialylation, N-glycan core and peripheral fucosylation, as well as the presence of biantennary as well as branched complex-type N-glycans. However, specificities of individual plant lectins towards terminal mono-or oligosaccharide epitopes are usually not well characterized and may have multiple interpretations. Therefore the previous lectin studies gave a useful impression of the terminal monosaccharide epitopes but not exact structural information. The present structural data including larger oligosaccharide structures could have parallels with the prior data, but one should also consider technical factors which may explain differences or potentially cause artificial similarities. These differences include e.g. cell culture conditions, assay techniques, sample preparation, and differences between the studied cell lines. For example, we have previously shown that minor cell culture reagents may cause glycan contamination of stem cells .
By employing rapid purification and direct analysis of non-derivatized glycans we demonstrated mass spectrometric N-glycan profiling of the scarce hESC samples, enabling analysis of samples as small as 100 000 cells. In many glycomic studies of mammalian cells and tissues the isolated glycans have been derivatized (e.g. permethylated) prior to mass spectrometric profiling [45–48] or chromatographic analysis . However, we chose to directly analyze the picomolar quantities of unmodified glycans and high sensitivity was achieved while omitting the derivatization and the subsequent additional purification steps. This straightforward method could be widely applicable to analysis and monitoring of stem cell lines. We have recently applied the same method to human cord blood hematopoietic cells  as well as human mesenchymal stem cells .
Stem cell glycosylation has been reported to be sensitive to composition of the cell culture medium [20, 21]. In the present study, we analyzed all biological material in contact with the cells to exclude potential contamination sources. Since no cell type in the present study had identical cell culture conditions, we could not exclude the possibility that the observed profile differences were in part influenced by differences in cell culture. However, our data supports the conclusion that the major identified N-glycan structural features were not dependent on changes in either cell culture media or growth surface. hESCs and EBs were grown in the same culture medium except that bFGF was omitted from EB culture, while the major difference between hESC and EB culture was that hESC colonies were grown on feeder cells and EBs in suspension. To analyze if different growth surfaces could affect cellular glycosylation, we have compared N-glycan profiles of hESCs grown on hEF, mEF, Matrigel, and a defined non-animal growth support (MM et al., manuscript in preparation). On all these surfaces, hESCs show an N-glycan profile typical to undifferentiated cells, including abundant complex fucosylation (such as S1H5N4F2, Fig. 3A) and low terminal HexNAc (such as S1H5N5F1, Fig. 3B). This suggests that the identified hESC-associated N-glycan profile features are not sensitive to changes in the growth surface. In addition, human fibroblast feeder cells, grown together with hESC, produce an N-glycan profile missing the key identifying characteristics of hESC glycosylation (for example complex fucosylation, Fig. 3A). Further, stage 3 differentiated cells grown as monolayers in different culture medium expressed the same differentiated cell associated structures as EBs (Fig. 1, 2, 3).
Human embryonic stem cells have a characteristic N-glycan profile which undergoes major changes when the cells differentiate. Information regarding the specific glycan structures may be utilized in developing reagents for targeting these cells and their progeny. Future studies investigating the developmental and molecular regulatory processes resulting in the observed N-glycan profiles may provide significant insight into mechanisms of human development and regulation of glycosylation.
Human embryonic stem cell lines
Finnish hESC lines FES 21, FES 22, FES 29, and FES 30 were cultured as described previously . Briefly, two of the analysed cell lines were initially derived and cultured on mouse embryonic fibroblast (mEF) feeders, and two on human foreskin fibroblast (hEF) feeder cells. For the present studies all of the lines were transferred on hEF feeder cells and cultured in serum-free medium supplemented with Knockout serum replacement (Gibco). To induce the formation of embryoid bodies (EB) the hESC colonies were first allowed to grow for 10–14 days whereafter the colonies were cut in small pieces and transferred on non-adherent Petri dishes to form suspension cultures. The formed EB were cultured in suspension for the next 10 days in standard culture medium without bFGF. For further differentiation (into stage 3 differentiated cells, St.3) EB were transferred onto gelatin-coated culture dishes in media supplemented with insulin-transferrin-selenium and cultured for 10 days.
For N-glycan profiling, on average 100 000 cells were collected mechanically from culture on hEF feeder cell layers, washed five times with phosphate buffered saline, and stored frozen until the analysis. In fluorescence-assisted cell sorting (FACS) analyses 70–90% of cells from mechanically isolated hESC colonies were typically Tra 1–60 and Tra 1–81 positive (data not shown). The differentiation protocol favours the development of neuroepithelial cells while not directing the differentiation into distinct terminally differentiated cell types . Stage 3 cultures consisted of a heterogeneous population of cells dominated by fibroblastoid and neuronal morphologies. For more detailed structural analyses by NMR spectroscopy and glycosidase digestions, up to 10 million hESC were grown on mEF layers.
Asparagine-linked glycans were detached from cellular glycoproteins by F. meningosepticum peptide:N-glycosidase F digestion (Calbiochem) and purified as described previously . Briefly, the detached glycans were purified by sequential precipitation/extraction and solid-phase extraction steps with miniaturized chromatography columns of C18 silica, strong cation-exchange resin, porous graphitized carbon, and for the sialylated glycans also microcrystalline cellulose.
Mass spectrometry and data analysis
MALDI-TOF mass spectrometry was performed with a Bruker Ultraflex TOF/TOF instrument (Bruker Daltonics, Germany) essentially as described . Relative signal intensities of neutral and sialylated glycan components were assigned based on their relative signal intensities in the mass spectra when analyzed separately as the neutral and sialylated N-glycan fractions [52–55]. We calculated relative intensities for all detected glycan signals using the Flexanalysis 3.0 software (Bruker Daltonics). The present glycan profiles were extracted from the resulting signal lists by removing the effect of isotopic pattern overlapping, multiple alkali metal adduct signals, products of elimination of water from the reducing oligosaccharides, and other interfering mass spectrometric signals not arising from the original glycans in the sample. The resulting glycan signals in the presented glycan profiles were normalized to 100% to allow comparison between samples. The relative amounts of each glycan signal are expressed as "% of total profile" similarly as previously reported [17, 18, 50]. The mass spectrometric fragmentation analysis of permethylated glycans was performed using the Bruker Ultraflex TOF/TOF instrument according to manufacturer's instructions.
Aliquots from the N-glycan fractions were subjected to digestion with α-mannosidase from Jack beans (C. ensiformis; Sigma-Aldrich); β1,4-galactosidase, β-N-acetylglucosaminidase, and α2,3-sialidase from S. pneumoniae; broad-range sialidase from A. ureafaciens; and β1,3-galactosidase, α1,3/4-fucosidase, and α1,2-fucosidase from X. manihotis (all from Calbiochem). Reactions with approximately 1–10 pmol oligosaccharide aliquots were carried out by overnight digestion at +37°C in 10 μl of 50 mM sodium acetate buffer pH 5.5. The activities of the enzymes in each reaction were optimised such that they had the following substrate specificities in control reactions: α2,3-sialidase digested Neu5Acα2-3Galβ1-4GlcNAc (Neu5Acα2-3LacNAc) but not Neu5Acα2-6LacNAc; β-N-acetylglucosaminidase digested GlcNAcβ1-3LacNAc but not GalNAcβ1-4GlcNAc; LacNAc but neither Galβ1-3GlcNAc nor Galα1-3LacNAc were digested with β4Gal; β1,3-galactosidase digested Galβ1-3GlcNAc; α1,3/4-fucosidase digested Fucα1-3(Galβ1-4)GlcNAc (Lex) but not Fucα1-2Galβ1-3GlcNAc; α1,2-fucosidase digested Fucα1-2Galβ1-3GlcNAc but not Lex; and α-mannosidase digested the high-mannose type N-glycans in a standard human N-glycan mixture. Digested glycan fractions were purified for analysis by solid-phase extraction and analyzed by mass spectrometry as described above.
The isolated glycans were purified for the analysis by gel filtration high-pressure liquid chromatography in a column of Superdex peptide HR 10/30 (Amersham), with water (neutral glycans) or 50 mM NH4HCO3 (sialylated glycans) as the eluant at a flow rate of 1 ml/min. The eluant was monitored at 214 nm. Oligosaccharide pools were quantified against external standards N-acetylglucosamine and N-acetylneuraminic acid. Prior to NMR analysis the purified glycome fractions were repeatedly dissolved in 99.996% deuterium oxide and dried to omit H2O and to exchange sample protons. The 1H NMR spectra at 800 MHz were recorded using a cryo-probe for enhanced sensitivity .
Fluorescein-labeled lectins used in lectin binding studies were from EY Laboratories. Specificity of binding was controlled by inhibition with 50 mM α3'-sialyllactose (Kyowa Hakko Kogyo, Japan), 100 mM α-D-mannose methyl glycoside (Sigma-Aldrich), and 100 mM L-fucose (Danisco Sweeteners, Finland) for Maackia amurensis agglutinin (MAA), Pisum sativum agglutinin (PSA), and Ulex europaeus agglutinin-I (UEA-I), respectively. Fluorescence-assisted cell sorting (FACS) analyses were performed essentially as described .
Normalized mass spectrometric N-glycan profile data from hESC (n = 4), EB (n = 7), and stage 3 differentiated cells (n = 6) were imported to Statistica 7.0 software (StatSoft). If all or almost all data values were zero, the corresponding signal was removed from the data set. In one way ANOVA with Fisher LSD post hoc analysis and Factor analysis signals having all or most of the values zero in certain cell type were omitted. Whisker box plots with means and standard deviations were developed and screened to have an overall view of the data and to identify mass peaks with variation between different cell lines or differentiation stage. Factor analysis was performed by principal component extraction; factor loadings were Varimax normalized and signals having factor loadings >0.62 and factors explaining >5% of variance were included into the model. Pearson correlation analysis was performed and correlations more than 0.7 or less than -0.7 were considered significant.
- Abbreviations are as follows:
bFGF: basic fibroblast growth factor
fluorescence-assisted cell sorting
human embryonic stem cells
human foreskin fibroblast
- H type 2:
Lewis x, Galβ1-4(Fucα1-3)GlcNAc
Lewis y, Fucα1-2Galβ1-4(Fucα1-3)GlcNAc
Maackia amurensis agglutinin
matrix-assisted laser desorption-ionization time-of-flight
mouse embryonic fibroblast
mouse embryonic stem cells
nuclear magnetic resonance
Pisum sativum agglutinin
sialyl Lewis x, Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAc
further (stage 3) differentiated cells
Ulex europaeus agglutinin-I. The schematic representation of oligosaccharides is in accordance with the guidelines proposed by the Consortium for Functional Glycomics http://www.functionalglycomics.org and as described in the legend of Figure 1.
Shriver Z, Raguram S, Sasisekharan R: Glycomics: a pathway to a class of new and improved therapeutics. Nat Rev Drug Discov. 2004, 3: 863-873. 10.1038/nrd1521.
Varki A: Biological roles of oligosaccharides: all of the theories are correct. Glycobiology. 1993, 3: 97-130. 10.1093/glycob/3.2.97.
Apweiler R, Hermjakob H, Sharon N: On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta. 1999, 1473: 4-8.
Lowe JB: Glycosylation in the control of selectin counter-receptor structure and function. Immunol Rev. 2002, 186: 19-36. 10.1034/j.1600-065X.2002.18603.x.
Fukuda M: Roles of mucin-type O-glycans in cell adhesion. Biochim Biophys Acta. 2002, 1573: 394-405.
Dell A, Morris HR, Easton RL, Patankar M, Clark GF: The glycobiology of gametes and fertilization. Biochim Biophys Acta. 1999, 1473: 196-205.
Handel TM, Johnson Z, Crown SE, Lau EK, Proudfoot AE: Regulation of protein function by glycosaminoglycans – as exemplified by chemokines. Annu Rev Biochem. 2005, 74: 385-410. 10.1146/annurev.biochem.72.121801.161747.
Helenius A, Aebi M: Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem. 2004, 73: 1019-1049. 10.1146/annurev.biochem.73.011303.073752.
Helenius A, Aebi M: Intracellular functions of N-linked glycans. Science. 2001, 291: 2364-2369. 10.1126/science.291.5512.2364.
Kornfeld S: Trafficking of lysosomal enzymes in normal and disease states. J Clin Invest. 1986, 77: 1-6. 10.1172/JCI112262.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts. Science. 1998, 282: 1145-1147. 10.1126/science.282.5391.1145.
Wobus AM, Boheler KR: Embryonic stem cells: prospects for developmental biology and cell therapy. Physiol Rev. 2005, 85: 635-678. 10.1152/physrev.00054.2003.
Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D: Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983, 2: 2355-2361.
Badcock G, Pigott C, Goepel J, Andrews PW: The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 1999, 59: 4715-4719.
Muramatsu T, Muramatsu H: Carbohydrate antigens expressed on stem cells and early embryonic cells. Glycoconj J. 2004, 21: 41-45. 10.1023/B:GLYC.0000043746.77504.28.
Wada Y, Azadi P, Costello CE, Dell A, Dwek RA, Geyer H, Geyer R, Kakehi K, Karlsson NG, Kato K: Comparison of the methods for profiling glycoprotein glycans – HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study. Glycobiology. 2007, 17: 411-422. 10.1093/glycob/cwl086.
Aoki K, Perlman M, Lim JM, Cantu R, Wells L, Tiemeyer M: Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo. J Biol Chem. 2007, 282: 9127-9142. 10.1074/jbc.M606711200.
Hemmoranta H, Satomaa T, Blomqvist M, Heiskanen A, Aitio O, Saarinen J, Natunen J, Partanen J, Laine J, Jaatinen T: N-glycan structures and associated gene expression reflect the characteristic N-glycosylation pattern of human hematopoietic stem and progenitor cells. Exp Hematol. 2007, 35: 1279-1292. 10.1016/j.exphem.2007.05.006.
Kyselova Z, Mechref Y, Kang P, Goetz JA, Dobrolecki LE, Sledge GW, Schnaper L, Hickey RJ, Malkas LH, Novotny MV: Breast cancer diagnosis and prognosis through quantitative measurements of serum glycan profiles. Clin Chem. 2008, 54: 1166-1175. 10.1373/clinchem.2007.087148.
Martin MJ, Muotri A, Gage F, Varki A: Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med. 2005, 11: 228-232. 10.1038/nm1181.
Heiskanen A, Satomaa T, Tiitinen S, Laitinen A, Mannelin S, Impola U, Mikkola M, Olsson C, Miller-Podraza H, Blomqvist M: N-glycolylneuraminic acid xenoantigen contamination of human embryonic and mesenchymal stem cells is substantially reversible. Stem Cells. 2007, 25: 197-202. 10.1634/stemcells.2006-0444.
Staudacher E, Altmann F, Wilson IB, Marz L: Fucose in N-glycans: from plant to man. Biochim Biophys Acta. 1999, 1473: 216-236.
Becker DJ, Lowe JB: Fucose: biosynthesis and biological function in mammals. Glycobiology. 2003, 13: 41R-53R. 10.1093/glycob/cwg054.
Skottman H, Mikkola M, Lundin K, Olsson C, Stromberg AM, Tuuri T, Otonkoski T, Hovatta O, Lahesmaa R: Gene expression signatures of seven individual human embryonic stem cell lines. Stem Cells. 2005, 23: 1343-1356. 10.1634/stemcells.2004-0341.
Venable A, Mitalipova M, Lyons I, Jones K, Shin S, Pierce M, Stice S: Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces. BMC Dev Biol. 2005, 5: 15-10.1186/1471-213X-5-15.
Wearne KA, Winter HC, O'Shea K, Goldstein IJ: Use of lectins for probing differentiated human embryonic stem cells for carbohydrates. Glycobiology. 2006, 16: 981-990. 10.1093/glycob/cwl019.
Wearne KA, Winter HC, Goldstein IJ: Temporal changes in the carbohydrates expressed on BG01 human embryonic stem cells during differentiation as embryoid bodies. Glycoconj J. 2008, 25: 121-136. 10.1007/s10719-007-9064-x.
Atwood JA, Cheng L, varez-Manilla G, Warren NL, York WS, Orlando R: Quantitation by isobaric labeling: applications to glycomics. J Proteome Res. 2008, 7: 367-374. 10.1021/pr070476i.
Kornfeld R, Kornfeld S: Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985, 54: 631-664. 10.1146/annurev.bi.54.070185.003215.
Imperiali B, O'Connor SE: Effect of N-linked glycosylation on glycopeptide and glycoprotein structure. Curr Opin Chem Biol. 1999, 3: 643-649. 10.1016/S1367-5931(99)00021-6.
Kilpatrick DC: Animal lectins: a historical introduction and overview. Biochim Biophys Acta. 2002, 1572: 187-197.
Zanetta JP, Vergoten G: Lectin domains on cytokines. Adv Exp Med Biol. 2003, 535: 107-124.
Wang X, Inoue S, Gu J, Miyoshi E, Noda K, Li W, Mizuno-Horikawa Y, Nakano M, Asahi M, Takahashi M: Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice. Proc Natl Acad Sci USA. 2005, 102: 15791-15796. 10.1073/pnas.0507375102.
Smith PL, Myers JT, Rogers CE, Zhou L, Petryniak B, Becker DJ, Homeister JW, Lowe JB: Conditional control of selectin ligand expression and global fucosylation events in mice with a targeted mutation at the FX locus. J Cell Biol. 2002, 158: 801-815. 10.1083/jcb.200203125.
Gooi HC, Feizi T, Kapadia A, Knowles BB, Solter D, Evans MJ: Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains. Nature. 1981, 292: 156-158. 10.1038/292156a0.
Solter D, Knowles BB: Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci USA. 1978, 75: 5565-5569. 10.1073/pnas.75.11.5565.
Fenderson BA, Zehavi U, Hakomori S: A multivalent lacto-N-fucopentaose III-lysyllysine conjugate decompacts preimplantation mouse embryos, while the free oligosaccharide is ineffective. J Exp Med. 1984, 160: 1591-1596. 10.1084/jem.160.5.1591.
Mollicone R, Cailleau A, Oriol R: Molecular genetics of H, Se, Lewis and other fucosyltransferase genes. Transfus Clin Biol. 1995, 2: 235-242. 10.1016/S1246-7820(05)80089-8.
Nakayama F, Nishihara S, Iwasaki H, Kudo T, Okubo R, Kaneko M, Nakamura M, Karube M, Sasaki K, Narimatsu H: CD15 expression in mature granulocytes is determined by alpha 1,3-fucosyltransferase IX, but in promyelocytes and monocytes by alpha 1,3-fucosyltransferase IV. J Biol Chem. 2001, 276: 16100-16106. 10.1074/jbc.M007272200.
Niemelä R, Natunen J, Majuri ML, Maaheimo H, Helin J, Lowe JB, Renkonen O, Renkonen R: Complementary acceptor and site specificities of Fuc-TIV and Fuc-TVII allow effective biosynthesis of sialyl-TriLex and related polylactosamines present on glycoprotein counterreceptors of selectins. J Biol Chem. 1998, 273: 4021-4026. 10.1074/jbc.273.7.4021.
Abeyta MJ, Clark AT, Rodriguez RT, Bodnar MS, Pera RA, Firpo MT: Unique gene expression signatures of independently-derived human embryonic stem cell lines. Hum Mol Genet. 2004, 13: 601-608. 10.1093/hmg/ddh068.
Bhattacharya B, Miura T, Brandenberger R, Mejido J, Luo Y, Yang AX, Joshi BH, Ginis I, Thies RS, Amit M: Gene expression in human embryonic stem cell lines: unique molecular signature. Blood. 2004, 103: 2956-2964. 10.1182/blood-2003-09-3314.
Mikkola M, Olsson C, Palgi J, Ustinov J, Palomaki T, Horelli-Kuitunen N, Knuutila S, Lundin K, Otonkoski T, Tuuri T: Distinct differentiation characteristics of individual human embryonic stem cell lines. BMC Dev Biol. 2006, 6: 40-10.1186/1471-213X-6-40.
Sato N, Sanjuan IM, Heke M, Uchida M, Naef F, Brivanlou AH: Molecular signature of human embryonic stem cells and its comparison with the mouse. Dev Biol. 2003, 260: 404-413. 10.1016/S0012-1606(03)00256-2.
Dell A, Morris HR: Glycoprotein structure determination by mass spectrometry. Science. 2001, 291: 2351-2356. 10.1126/science.1058890.
Novotny MV, Mechref Y: New hyphenated methodologies in high-sensitivity glycoprotein analysis. J Sep Sci. 2005, 28: 1956-1968. 10.1002/jssc.200500258.
Sutton-Smith M, Morris HR, Grewal PK, Hewitt JE, Bittner RE, Goldin E, Schiffmann R, Dell A: MS screening strategies: investigating the glycomes of knockout and myodystrophic mice and leukodystrophic human brains. Biochem Soc Symp. 2002, 105-115.
Uematsu R, Furukawa J, Nakagawa H, Shinohara Y, Deguchi K, Monde K, Nishimura S: High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis. Mol Cell Proteomics. 2005, 4: 1977-1989. 10.1074/mcp.M500203-MCP200.
Callewaert N, Van VH, Van HA, Laroy W, Delanghe J, Contreras R: Noninvasive diagnosis of liver cirrhosis using DNA sequencer-based total serum protein glycomics. Nat Med. 2004, 10: 429-434. 10.1038/nm1006.
Heiskanen A, Hirvonen T, Salo H, Impola U, Olonen A, Laitinen A, Tiitinen S, Natunen S, Aitio O, Miller-Podraza H: Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage. Glycoconj J. 2008
Okabe S, Forsberg-Nilsson K, Spiro AC, Segal M, McKay RD: Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro. Mech Dev. 1996, 59: 89-102. 10.1016/0925-4773(96)00572-2.
Harvey DJ: Quantitative aspects of the matrix-assisted laser desorption mass spectrometry of complex oligosaccharides. Rapid Commun Mass Spectrom. 1993, 7: 614-619. 10.1002/rcm.1290070712.
Naven TJ, Harvey DJ: Effect of structure on the signal strength of oligosaccharides in matrix-assisted laser desorption/ionization mass spectrometry on time-of-flight and magnetic sector instruments. Rapid Commun Mass Spectrom. 1996, 10: 1361-1366. 10.1002/(SICI)1097-0231(199608)10:11<1361::AID-RCM642>3.0.CO;2-A.
Papac DI, Wong A, Jones AJ: Analysis of acidic oligosaccharides and glycopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 1996, 68: 3215-3223. 10.1021/ac960324z.
Saarinen J, Welgus HG, Flizar CA, Kalkkinen N, Helin J: N-glycan structures of matrix metalloproteinase-1 derived from human fibroblasts and from HT-1080 fibrosarcoma cells. Eur J Biochem. 1999, 259: 829-840. 10.1046/j.1432-1327.1999.00105.x.
Li YL, Wu GZ, Zeng L, Dawe GS, Sun L, Loers G, Tilling T, Cui S, Schachner M, Xiao ZC: Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLCγ and cooperate to modulate embryonic stem cell survival and proliferation. FEBS Lett. 2009, 583: 703-710. 10.1016/j.febslet.2009.01.013.
This work was supported by the Finnish Funding Agency for Technology and Innovation (TEKES). Derivation of the hESC lines was supported by grants from the Juvenile Diabetes Research Foundation, Sigrid Jusélius Foundation (TO) and the Academy of Finland (TT and TO).
TS and AH contributed equally to this work. AH performed N-glycosylation analyses and TS analyzed the data and drafted the manuscript. MM, CO, TT, and TO provided the hESC samples. AH, MB, JH, TS, and JS developed glycan analysis techniques for stem cells. TJ analyzed gene expression data. MM and MT performed lectin binding assays. OA and AO performed the NMR analysis. JN participated in writing the manuscript. JS and JL supervised the project and contributed equally to this work. All authors read and approved the final manuscript.
Tero Satomaa, Annamari Heiskanen, Juhani Saarinen and Jarmo Laine contributed equally to this work.
Electronic supplementary material
Additional file 1: . Supplementary data including the following supplementary figures and tables: Supplementary Figures 1 and 2. Examples of glycan profiling method evaluation. Supplementary Figure 3 and Supplementary Tables 1 and 2. NMR analysis of neutral and sialylated N-glycans. (PDF 204 KB)
Authors’ original submitted files for images
About this article
Cite this article
Satomaa, T., Heiskanen, A., Mikkola, M. et al. The N-glycome of human embryonic stem cells. BMC Cell Biol 10, 42 (2009). https://doi.org/10.1186/1471-2121-10-42
- Embryoid Body
- Glycan Structure
- hESC Line
- Undifferentiated hESC
- Glycan Profile