- Research article
- Open Access
Functional characterization of the trans-membrane domain interactions of the Sec61 protein translocation complex beta-subunit
BMC Cell Biology volume 10, Article number: 76 (2009)
In eukaryotic cells co- and post-translational protein translocation is mediated by the trimeric Sec61 complex. Currently, the role of the Sec61 complex β-subunit in protein translocation is poorly understood. We have shown previously that in Saccharomyces cerevisiae the trans-membrane domain alone is sufficient for the function of the β-subunit Sbh1p in co-translational protein translocation. In addition, Sbh1p co-purifies not only with the protein translocation channel subunits Sec61p and Sss1p, but also with the reticulon family protein Rtn1p.
We used random mutagenesis to generate novel Sbh1p mutants in order to functionally map the Sbh1p trans-membrane domain. These mutants were analyzed for their interactions with Sec61p and how they support co-translational protein translocation. The distribution of mutations identifies one side of the Sbh1p trans-membrane domain α-helix that is involved in interactions with Sec61p and that is important for Sbh1p function in protein translocation. At the same time, these mutations do not affect Sbh1p interaction with Rtn1p. Furthermore we show that Sbh1p is found in protein complexes containing not only Rtn1p, but also the two other reticulon-like proteins Rtn2p and Yop1p.
Our results identify functionally important amino acids in the Sbh1p trans-membrane domain. In addition, our results provide additional support for the involvement of Sec61β in processes unlinked to protein translocation.
The biosynthesis of secretory proteins requires translocation through the Sec61 channel into the endoplasmic reticulum (ER) . The protein conducting channel is formed by three proteins and is conserved in evolution from archaea and bacteria to eukaryotic cells [2, 3]. The largest subunit forming the actual protein conducting channel is named Sec61α in eukaryotes and SecY in bacteria and archaea. The β-subunit is termed Sec61β in metazoans and SecG in bacteria and archaea, whereas in yeast Saccharomyces cerevisiae (S. cerevisiae) two homologous β-subunits, Sbh1p and Sbh2p exist. The γ-subunit is called Sec61γ in mammalian cells, Sss1p in S. cerevisiae cells and SecE in bacteria and archaea. In S. cerevisiae two homologous trimeric Sec61 complexes exist. The Sec61 complex is composed of Sec61p, Sbh1p and Sss1p [4–7]. This complex together with Sec63p and Kar2p functions in co-translational translocation . In association with Sec62p, Sec63p, Sec71p and Sec72p, Sec61 complex mediates also post-translational protein translocation as the so-called Sec complex [6, 9]. In addition to its role in co- and post-translational translocation, the Sec61 complex has also been implicated in retro-translocation of misfolded proteins to the cytosol for degradation [10, 11]. The second S. cerevisiae translocation complex, the Ssh1 complex, consists of the Sec61p homologue Ssh1p, the Sbh1p homologue Sbh2p, and Sss1p [2, 8]. The Ssh1 complex has been shown to contribute to both co- and post-translational translocation and ER associated degradation (ERAD) [4, 12].
Recent structural studies have shed light on the assembly and function of the α-subunit as the protein conducting channel [3, 13, 14]. In contrast, the functions of the β- and γ-subunits remain largely unclear. The β-subunit is not essential, but has a facilitating role in translocation in mammalian cells . In addition to its interactions with Sec61p, the mammalian β-subunit has been shown to interact with signal peptidase  and the ribosome . The yeast β-subunit, Sbh1p, was shown to act as a guanine nucleotide exchange factor for signal recognition particle receptor . The two yeast β-subunits, Sbh1p and Sbh2p are encoded by non-essential genes. Deletion of either gene alone has no effect on growth, while the deletion of both SBH1 and SBH2 results in temperature-sensitivity for growth [4, 7]. Different studies have reported variable effects of SBH1 and SBH2 deletion on protein translocation of the α mating pheromone precursor (ppαf), Kar2p, bacterial α-amylase and dipeptidyl aminopeptidase [4, 15, 18, 19]. These results indicate a role for Sbh1p in co-translational protein translocation. Interestingly, the Sbh1p tm-domain alone is sufficient to support in vivo co-translational protein translocation and for interactions with Sec61 both in S. cerevisiae and in S. pombe [18–20].
The non-essential role of SBH1 and SBH2, in contrast to the essential genes encoding the α- and γ-subunits Sec61p and Sss1p, suggests that the β-subunits may have functions that are not directly linked to protein translocation. Previously, a stabilizing role in the translocation complex has been suggested for both β- and γ-subunits . Both in mammalian and yeast cells, β-subunits have been shown to co-immunoprecipitate with the exocyst complex components Sec8p, Sec10p and Sec15p [22, 23] whose main function is thought to be tethering of transport vesicles to the plasma membrane . In addition, SBH1 over-expression has been shown to rescue the temperature-sensitive growth phenotype of several exocyst subunit mutations [22, 23].
It appears that a pool of Sbh1p exists outside Sec61 complex and that these Sbh1p proteins can interact with Rtn1p, a member of the reticulon family proteins . Rtn1p, together with Rtn2p and the DP1/Yop1p family protein Yop1p have been shown to function in modulation of the ER membrane structure [25–27]. Interestingly, in addition to its interactions with Sbh1p, Rtn1p has also been shown to co-purify with Exocyst subunit Sec6 . Collectively, the current data suggests that Sbh1p displays complex molecular interactions and that its function may not be restricted to protein translocation. In this study we characterize the molecular determinants that are important for the function of the Sec61 protein translocation complex β-subunit tm-domain function. In addition, we show that this β-subunit interacts with ER-resident reticulon protein complexes.
The yeast strains used are summarized in Table 1. Yeast cells were cultivated aerobically on plates or in shaker flasks at indicated temperatures essentially as described previously . All strains used are congenic to H304 (NY179) (from P. Novick, Yale University). Gene deletions and carboxyl-terminal tagging of proteins were achieved with the PCR-based method using pFA6a-kanMX4, pFA6a-natNT2, pYM-hphNT1, pYM1 and pYM20 as templates ( from Michael Knop, EMBL, Heidelberg). G418 (Invitrogen) was used at a concentration of 200 μg/ml, nourseothricin at 100 μg/ml (Werner BioAgents, Germany), and hygromycin (EMD Biosciences, Inc.) at 300 μg/ml. Yeast transformations were carried out as described previously . Complementation and suppression tests were performed as described previously .
SBH1 mutagenesis and library construction
Random mutagenesis for Sbh1p tm-domain encoding DNA segment was carried out by error-prone PCR using Goldstar DNA Polymerase (Eurogentec). PCR was performed in a 50 μl volume (30 cycles of 30 s at 94°C, 30 s at 56°C, and 1 min at 72°C), using 0.1 μg of template plasmid (YEpSBH1(50-75)  (Table 2), 100 pmol of each primer (5'-GCATTTCTAGAATGCTAAGAGTAGATCCCTTAG-3' and 5'-GCATTCTCGAGTTAAGAAATAACATGTAATGC-3'), 3 mM MgCl2, 0.5 mM MnCl2, three dNTPs at 0.12 mM, and the fourth at 0.016 mM and 2 units of Goldstar polymerase. Amplification products were gel-purified, pooled, digested with XbaI and Xhol restrictions enzymes and ligated into identically cut pVT102U . Approximately 10,000 colonies were obtained, scraped into medium and grown overnight at 37°C followed by isolation of the plasmid DNA. After transformation of strain H3232 with this library, approximately 300 transformants were picked and regrown overnight at 30°C as patches on SC-Ura plates. These plates were then replicated to 30°C or 38.5°C and their growth was followed for 3 days. Site-directed mutagenesis was performed by QuickChange protocol (Stratagene) using appropriate mutagenic primers and Phusion DNA polymerase (Finnzymes). All constructs were verified by sequencing.
Standard DNA methods were used. Oligonucleotides were purchased from Sigma Genosys or Invitrogen. The plasmids used for gene expression in yeast are listed in Table 2. YEpSBH1(1-82) (YEpSBH1) and YEpSBH1(50-75) (YEpSBH1-TM) have been described previously . SBH1 containing BIO-tag was constructed by amplifying with BamHI/XhoI containing oligonucleotides a fragment encoding the biotin acceptor peptide (BIO-tag) from Propionibacterium shermanii transcarboxylase . This fragment was added in front of SBH1 mutants (obtained from the library screen) by nested PCR using pFA6a-HBH-kanMX46 ( from Peter Kaiser, University of California) as the template for BIO-tag. Generated fragments were cloned into p426ADH plasmid . All constructs generated by PCR were sequenced.
The Sec61p and Sbh1p antibodies have been described previously . Monoclonal anti-HA antibody (12CA5) was purchased from Roche Applied Science. Monoclonal anti-myc (9E10) antibody was obtained from Kristiina Takkinen (VTT Technical Research Centre of Finland). Antibody dilutions used in Western blotting were 1:2000 for anti-HA, 1:1000 for anti-myc, anti-Sbh1p, 1:4000 and 1:2000 for anti-Sec61p. HRP-conjugated goat anti-mouse and anti-rabbit antibodies (Bio-Rad) were diluted 1:2000.
Pull-downs and immunoprecipitations
Membrane protein-enriched fractions were isolated as described  and used for pull-down of BIO-tagged or immunoprecipitation of HA-tagged proteins. Streptavidin or anti-HA (12CA5) antibody-coupled Dynabeads (Dynal) were used for precipitation of BIO-tagged Sbh1p proteins or Rtn1p-3HA and Rtn2p-3HA. Coupling of anti-HA antibodies to Protein G-coupled magnetic beads was carried out according to manufacturer's instructions (Dynal). Incubation with the lysate was carried out at 4°C by end over end rotation for 1 h followed by 5 washes at 4°C by rotation, 15 min each. The bound proteins were eluted twice with 0.1% SDS treatment for 5 min at 95°C. The eluates were adjusted to 100 mM DTT, heated as described above, and subjected to SDS-PAGE and Western blotting. The protein bands were detected with specific antibodies or HRP-conjugated streptavidin (Molecular Probes) diluted 1:5000 and visualized with the ECL detection system (Amersham Biosciences).
Ubiquitin-assisted Translocation Assay (UTA)
Plasmids YEpSBH1-FL(DM)L (full length SBH1 with mutations P54S V57G), YEpSBH1-TM(DM)L (tm-domain encoding SBH1 with mutations P54S V57G), YEpSBH1-TM(SM1)L (tm-domain encoding SBH1 with mutation P54S) and YEpSBH1-TM(SM2)L (tm-domain encoding SBH1 with mutation V57G) were transformed into H3543 strain containing plasmids encoding either Suc223, Sec2277, Dap2300 (from N. Johnsson, University of Muenster, Germany, and J. Brown, Newcastle University, United Kingdom), or the empty vector pRS314 [1, 36]. Patches of transformants grown on SC-Trp-Leu were replicated on SC-Trp-Leu-Ura plates and their growth was followed for 3 days at 24°C.
Characterization of Sbh1p trans-membrane domain mutants
We have previously shown that the Sbh1p tm-domain is capable of interacting with the Sec61p-Sss1p complex and sufficient to rescue the co-translational translocation defect in sbh1Δ sbh2Δ cells . In order to identify regions in the Sbh1p tm-domain that are important for its function we generated, by error-prone PCR, a DNA library containing random mutations in the tm-domain encoding fragment. This library was transformed into the temperature-sensitive sbh1Δ sbh2Δ cells, approximately 300 transformants were picked, and their capability to grow at the restrictive temperature was monitored. Approximately 30% of the transformants could not grow at 38.5°C, indicating that the Sbh1p tm-domain had acquired mutations in functionally important amino acids. The plasmids from 60 non-functional and functional clones were subjected to sequencing. This revealed that most of the plasmids (93%) contained mutations that resulted in one or more amino acid changes within the Sbh1p tm-domain. The sbh1 mutants, which could not rescue temperature-sensitivity in sbh1Δ sbh2Δ cells, contained either an early stop codon or more than one amino acid changes. A selection of mutants is shown in Table 3. The results demonstrate that the Sbh1p tm-domain tolerates a surprising number of non-conservative amino acid changes without a significant effect on sbh1Δ sbh2Δ cell growth.
Recent studies have revealed high resolution SecY-complex structures from different species [3, 13, 14]. While no high resolution structure of the eukaryotic Sec61 translocation channel exists, a lower resolution structure of the mammalian non-translating ribosome-bound Sec61 complex was recently obtained . This structure, however, does not reveal details of the β-subunit. The Sec61 β homologue SecG is the least conserved subunit among SecY complexes. In some species such as Thermotoga maritima SecG is comprised of two trans-membrane helices, whereas the structure of Methanococcus jannaschii trimeric SecY translocation complex reveals that the β-subunit traverses the membrane with a single α-helix with one side of the helix interacting with the α-subunit of the complex (Figure 1A and 1B, ).
In order to understand where the obtained mutations are located in the Sbh1p tm-domain structure, we made use of the similarity of SecG tm-domains from different species and deduced a model for Sbh1p association with Sec61α (Figure 1C and 1D). The tm-domains of Sbh1p homologues have a highly conserved proline residue at the membrane/cytosol boundary complex . We used this residue to align the Sbh1p tm-domain with the Methanococcus jannaschii SecG tm-domain (Figure 1C). The M. jannaschii SecG tm-domain has one side of its tm domain facing the tm-domains 1 and 4 of SecY (Figure 1B). To test the effect of mutations in different locations of the Sbh1p tm-domain α-helix, eight mutants were selected for further analysis (Figure 1C). Based on the model, mutations in TM-1, TM-2, TM-3 and TM-4 are mostly oriented away from the Sec61 whereas mutations in TM-5, TM-6, TM-7 and TM-8 are facing Sec61 (Figure 1C). When tested for their capability to rescue the temperature-sensitivity of the sbh1Δ sbh2Δ cells, mutants TM-5, TM-6, TM-7, and TM-8 were unable to rescue temperature-sensitivity of this strain. Mutant TM-3 was partially functional and mutants TM-1, TM-2 and TM-4 displayed normal growth (Figure 2A).
The Sbh1p tm-domain is sufficient for co-purification with the two other subunits of the Sec61 complex, Sec61p and Sss1p . We next tested the effect of the selected mutations in the tm-domain for interaction with Sec61. As the tm-domain is only 25 amino acids long and not recognized by the antibodies available, for detection and pull-down experiments, we made use of the BIO-tag derived from Propionibacterium shermanii transcarboxylase that is biotinylated in vivo in yeast [33, 34]. Lysates were prepared from sbh1Δ cells that expressed, from a plasmid, different mutant variants of Sbh1p tm-domain with a BIO-tag. These lysates were subjected to pull-down experiments with streptavidin-coupled magnetic beads followed by SDS-PAGE and Western blotting and detection with antibodies to Sec61p or streptavidin conjugated HRP (Figure 2B). The results show that the three mutants (mutants TM-5, TM-6 and TM-7) that could not rescue the temperature-sensitive phenotype of sbh1Δ sbh2Δ cells, lose their interaction with Sec61p. The TM-8 mutant protein levels were very low (data not shown) and were excluded from this analysis. At the same time mutant TM-2 co-purified Sec61 as efficiently as the wt Sbh1p tm-domain. Mutant TM-1 and TM-3 displayed reduced interaction with Sec61. Mutant TM-4 that could rescue temperature-sensitivity of sbh1Δ sbh2Δ cells could only weakly copurify Sec61 (Figure 2B).
The double mutation P54S V57G inactivates the Sbh1p trans-membrane domain
Sequence homology between tm-domains of MjSecG and Sbh1p and our mutant collection suggest that mutations D53G, P54S, V57G, F64S, S67P, and V69A could affect potentially important positions within the Sbh1p tm-domain. D53 is located at the cytosol/membrane boundary whereas P54, V57 and F64 are conserved between M. jannaschii and S. cerevisiae. However, the single mutations D53G, P54S, V57G, F64S, S67P and V69A did not inactivate the Sbh1p tm-domain as judged by the rescue of the temperature-sensitivity of sbh1Δ sbh2Δ cells (Table 3, Data not shown). Based on the data indicating that one side of the predicted Sbh1p tm-domain α-helix is especially sensitive for mutations, and on the sequence conservation within the Sbh1p homologues, we generated, by site directed mutagenesis, versions of Sbh1p that have P54S V57G mutations (Figure 3A). These mutations target the side of Sbh1p tm-domain α-helix that based on the SecY structure  would face Sec61p (Figure 1A and Figure 3A). When expressed in sbh1Δ sbh2Δ cells this double mutant (DM) was unable to rescue the temperature-sensitive growth phenotype at 38.5°C whereas the single mutants P54S (SM1) and V57G (SM2) were as efficient as the wild type SBH1 to rescue the temperature-sensitive growth of sbh1Δ sbh2Δ cells (Figure 3B). The same was true when the BIO-tagged versions of SBH1 were tested in this experimental setup (Figure 3B lower panel). Both untagged and BIO-tagged full length (FL) Sbh1p containing both P54S and V57G mutations in the tm-domain were able to rescue sbh1Δ sbh2Δ cell growth at the restrictive temperature. This suggests that the cytosolic domain of Sbh1p contributes to Sbh1p function or interaction with Sec61 enables sufficient in vivo functionality of Sbh1p that has these mutations in the tm-domain.
Sbh1p has previously been implicated in co-translational translocation (). We therefore tested whether the P54S V57G double mutant is functional in protein translocation using the UTA assay [36, 38]. The UTA assay measures coupling of translation to translocation by utilizing reporter proteins which contain a yeast signal sequence, a "spacer" peptide, ubiquitin and finally Ura3p. When translation is closely coupled to translocation, the reporter protein fusion with Ura3p is translocated to the lumen of the ER before the ubiquitin folds and is cleaved by cytosolic proteases. Such cells can not survive without uracil in the growth medium as Ura3p needs to be in the cytosol to rescue lack of uracil in the growth medium. In cases where there is a delay between translation and translocation, ubiquitin folds, gets cleaved and Ura3p is released in the cytosol enabling growth in the absence of uracil. Thus, if Sbh1 mutant proteins expressed in sbh1Δ sbh2Δ cells can not support translocation of the Ura3p reporter protein into the ER lumen the cells should be able to grow in the absence of uracil in the growth medium. This would indicate that this Sbh1p mutant is inactivated for protein translocation. To test this sbh1Δ sbh2Δ leu2-3,112 ura3-52 trp1Δ cells were transformed with reporter plasmids encoding invertase or dipeptidyl aminopeptidase B (Dap2) signal sequence and 23 (Suc223), 277 (Suc2277) or 300 (Dap2300) first amino acids, respectively, before the ubiquitin moiety and Ura3p. The same cells were also transformed with plasmids encoding Sbh1p tm-domain with single P54S (TM-SM1) or V57G (TM-SM2) or the P54S V57G double mutation (TM-DM) and with a plasmid encoding full length Sbh1p with the P54S V57G double mutation (FL-DM) or the empty vector. Suc2p uses the post-translational and Dap2p the co-translational translocation pathway . The sbh1Δ sbh2Δ cells expressing Suc223 grew in all cases in the absence of uracil in the growth medium as the 23 amino acid linker enables rapid folding of the ubiqutin moiety, its cleavage and release of the Ura3p in the cytosol (Figure 3C, lower panel). The sbh1Δ sbh2Δ cells expressing Suc2277 did not grow in the absence of uracil indicating efficient post-translational translocation of this reporter protein in to the ER lumen in the absence of Sbh1 and Sbh2 proteins. Dap2300 reporter expressing sbh1Δ sbh2Δ cells also grew in the absence of uracil (vector, Figure 3C, lower panel) indicating a defect in co-translational translocation as previously reported [18, 19]. The defect in co-translational translocation was rescued by reintroduction of full length Sbh1p FL-DM, Sbh1p TM SM1 or Sbh1p TM SM2, but not by expression of the Sbh1p tm-domain with the P54S V57G double mutation (TM-DM) (Figure 3C, lower panel). We conclude that wt Sbh1p, and the Sbh1p tm-domain with the single P54S or V57G mutation can rescue the co-translational translocation defect in sbh1Δ sbh2Δ cells whereas the Sbh1p tm-domain with the double mutation P54S V57G cannot.
The Sbh1p P54S V57G trans-membrane domain double mutant does not bind to Sec61p
In order to test Sec61 binding of the Sbh1p tm-domain double mutant we made use of constructs which enabled expression of the point mutants with an amino-terminal BIO tag (Figure 3B, lower panel). These constructs or an empty vector were transformed into sbh1Δ RTN1-3HA cells, lysates were prepared and subjected for pull-downs with streptavidin coated beads. The pull-downs were analyzed by SDS-PAGE and Western blotting with antibodies against Sec61p, HA or with HRP-coupled streptavidin. The results show that although similar amounts of Sbh1TM-DM and Sbh1TM were pulled down with streptavidin coated beads, the trans-membrane domain with P54S V57G mutations interacted only weakly with Sec61 (Figure 3D). At the same time, Sec61p co-precipitated with full length Sbh1p, Sbh1p tm-domain and full length Sbh1p FL(DM). The P54S V57G mutations did not affect co-precipitation of Sbh1p with Rtn1p-HA (Figure 3D). These results support the growth rescue and protein translocation experiment results and suggests that P54 V57 mutations affect interaction of the Sbh1 tm-domain with Sec61p. When these mutations are present in the full length Sbh1p containing also the cytosolic domain, the interaction of Sbh1p with Sec61p is partially restored. This suggests the cytosolic domain contributes to the Sbh1p interaction with Sec61.
The Sbh1p trans-membrane domain is sufficient for interaction with Rtn2p and Yop1p
Our previous results together with the results above showed that Sbh1p is found in a complex with Rtn1p and that the tm-domain is sufficient for this interaction (, Figure 3D). In addition to Rtn1p, S. cerevisiae expresses two ER-localized membrane proteins, Rtn2p and Yop1p that are implicated in ER structure regulation [25–27]. In order to test whether also Rtn2p and Yop1p are found in protein complexes with Sbh1p, yeast strains were generated where SBH1 was deleted and RTN1, RTN2 or YOP1 were tagged either with HA or myc tags at their genomic locus where their expression was maintained under their endogenous promoters. These cells were transformed with an empty plasmid or a plasmid expressing a BIO-tagged version of either the full length Sbh1p or the Sbh1p tm-domain. Cells were subjected to pull-down experiments with streptavidin coated beads and the precipitated material was analyzed after SDS-PAGE and Western blotting with anti HA or myc antibodies. When Sbh1p full length or the tm-domain was pulled-down from lysates expressing BIO-Sbh1p, BIO-Sbh1p-tm, Rtn1p-HA, or Rtn2p-myc, both Rtn1p-HA and Rtn2p-myc were detected in the precipitates together with Sec61p. In cells harboring the empty vector instead of BIO-Sbh1p, only a weak signal for these proteins was detected (Figure 4A, vector). In lysates prepared from a strain expressing BIO-Sbh1p, BIO-Sbh1p-tm, Rtn1p-HA, or Yop1p-myc, both Rtn1p-HA and Yop1p-myc proteins co-purified with Sbh1p (Figure 4B). These results suggest that Sbh1p interacts with a larger protein complex that appears to contain all yeast reticulon-homology proteins.
We previously showed that the tm-domain of the S. cerevisiae Sec61 β-subunit Sbh1p is sufficient to ensure viability of SBH1 SBH2 deleted cells, support co-translational translocation and interact with the α-subunit Sec61p . This indicates that the Sbh1p tm-domain possesses features that ensure specific functional interactions with the Sec61 protein translocation channel. Currently, no high resolution structure exists for the eukaryotic Sec61 complex and therefore it is not known how the β-subunit interacts with Sec61p. However, recently high resolution structures of the archaeal and prokaryotic SecY complexes have been resolved [3, 13, 14]. Furthermore, a lower resolution structure of the mammalian Sec61 complex bound to a non-translating ribosome was recently published . This structure generated by electron microscopy is similar to the archaeal M. jannashii SecY complex structure indicating high overall structural conservation between species. Despite these significant advances, the detailed function of the protein translocation complex and especially the functions of the small SecG/β and SecE/γ subunits have remained elusive.
Among the protein translocation channel subunits the different SecG/β subunits are the least conserved by sequence making it difficult to define critical amino acids for their function. In order to gain a better understanding of the molecular determinants that are responsible for the S. cerevisiae Sec61 β-subunit tm-domain in vivo function, we set out to identify mutations in the Sbh1p tm-domain that could abolish its interaction with Sec61 and that would affect co-translational protein translocation. We found that the Sbh1p tm-domain tolerates significant changes in its amino acid composition with remarkably small effects on its function. When we used helical wheel projections to predict the distribution of these mutations, a pattern for the localization of inactivating mutations emerged. Using the archaeal M. jannashii SecY complex structure as a model, we observed that mutations on the side of Sbh1p tm-domain that in SecG would face SecY were especially prone to inactive Sbh1p function. This we confirmed by generating a version of Sbh1p tm-domain containing two mutations that localized in the putative Sbh1p-Sec61p interaction interface. Sbh1p tm-domains with these (P54S V57G) mutations bound only weakly to Sec61p and were unable to support co-translational translocation in the UTA protein translocation assay (Figure 3). The P54S mutation changes a proline that is predicted to be located at the cytosol/membrane boundary. This mutation changes the Sbh1p tm-domain to resemble its close homologue in S. cerevisiae, Sbh2p, which naturally possesses a serine at this position [4, 7]. Sbh2p or its tm-domain alone have been shown to rescue the growth defect of sbh1Δ sbh2Δ cells [7, 18–20, 23]. This suggests that the V57G mutation is contributing significantly to inactivation of Sbh1p function and Sbh1p interaction with Sec61p. This conclusion is supported e.g. by mutations (V57A F64S) in the tm-domain mutant TM-23 (Table 3) which cause inactivation of Sbh1p. These mutations target the same side of the Sbh1p tm-domain alpha-helix like the inactivating mutations P54S and V57G. Both P54 and V57 are well conserved among SecG and Sec61 β homologues . When P54S V57G mutations were generated in full length Sbh1p, co-translational translocation was rescued sufficiently for cell survival at elevated temperature and the interaction with Sec61p in immunoprecipitations was partially restored. It is therefore possible, that the cytosolic part of Sbh1p contributes to Sec61 binding or Sec61 protein functionality either directly or indirectly through association of Sec61p, the ribosome or other components involved.
The interactions displayed by the cytosolic domain are poorly understood as even in the solved high resolution structures of SecYEG complexes, these domains are poorly resolved [3, 14]. One potential interaction partner could be the closely situated amino-terminal part of Sec61p [3, 37]. Additional interaction partners could be provided by the reported binding of Sec61 complex subunits with the oligosaccharyl transferase complex . Ultimately, a high resolution structure of the Sec61 complex is required to resolve the details of molecular interactions within this complex. However, our functional analysis of the Sbh1p tm-domain indicates the side of the Sec61 β-subunit tm-domain that is involved in Sec61p binding in S. cerevisiae. This suggests that the Sec61 β-subunit interaction with the Sec61 α-subunit is indeed well conserved in evolution. This conclusion is supported by the recent results showing that both the mammalian and S. pombe Sec61 β-subunits can complement for loss of Sbh1p and Sbh2p in S. cerevisiae .
We have shown previously that Sbh1p interacts with Rtn1p, a member of the reticulon family of proteins in yeast . This interaction appears to take place between Sbh1p and Rtn1p outside the Sec61 complex . Rtn1p and reticulon family proteins in general have been shown to play an important role in modulation of ER membrane tubulation both in S. cerevisiae and mammalian cells [25–27]. Additional components in this function in yeast are the second reticulon family protein Rtn2p and a member of the DP1/Yop1p family, Yop1p. Yop1 and reticulon proteins Rtn1 and Rtn2 have been shown to form complexes of reduced lateral mobility in the peripheral ER in S. cerevisiae [25, 26]. Here we show that Sbh1p co-immunoprecipitates not only with Rtn1p, but also with Rtn2p and Yop1p. Our findings support the recent results showing that Rtn1p, Rtn2p and Yop1p form trimeric complexes [25–27]. The role of Sbh1p interaction with reticulon and Yop1 complexes is currently unclear. However, the mutations that affected Sbh1p interaction with Sec61p did not abolish interaction with Rtn1p.
We have previously shown that overexpression of SBH1 can specifically suppress the growth defect of several mutations in the exocyst complex [7, 23]. In addition to these genetic interactions, Sbh1p also co-immunoprecipitates with exocyst subunits Sec15p and Sec8p in yeast cells . Intriguingly, similar interactions and functional links were obtained in mammalian cells  indicating that the Sec61 β and exocyst complex interactions carry out a conserved function. Association of the exocyst complex with ER membrane components is further supported by data showing co-purification of the exocyst complex with Rtn1p . The existence of a functional link between Rtn1p and the exocyst is also supported by the capability of RTN1 overexpression to suppress temperature-sensitive mutations in exocyst subunits Sec5p and Sec8p and induction of a cold sensitive phenotype in sec15-1 cells by RTN1 deletion (Zhao and Jantti, unpublished). Interestingly, recent results show a molecular interplay between Sec5p, TBK1 kinase, a preferentially ER-localized trans-membrane protein STING and Sec61 β in mammalian cells. STING and TBK1 are implicated in innate immunity regulation and it was proposed that STING and the protein translocation channel are functionally linked during innate immunity signaling . The exocyst subunit Sec5p has also previously been shown to be involved in innate immunity through its interaction with TBK1 kinase and knock-down of the exocyst subunit Sec5p renderes cells defective for STING function [40, 41]. These results together with our present data on Sec61 β-subunit interactions with reticulon/Yop1 protein complexes, suggest a complex molecular interplay between ER membrane proteins and the exocyst complex, possibly through interactions between the Sec61 β-subunit, the exocyst complex and reticulon proteins. The elucidation of these interactions and their role in the cell represents an interesting, yet demanding challenge for future studies.
Our results support the existence of significant structural and functional conservation in Sec61 β/SecG subunits and show that Sec61 β in S. cerevisiae associates with reticulon protein complexes. Furthermore, these results suggest an interesting role for Sec61 β in cellular regulation in addition to its role in protein translocation.
Johnson AE, van Waes MA: The translocon: a dynamic gateway at the ER membrane. Annu Rev Cell Dev Biol. 1999, 15: 799-842. 10.1146/annurev.cellbio.15.1.799.
Osborne AR, Rapoport TA, van den BB: Protein translocation by the Sec61/SecY channel. Annu Rev Cell Dev Biol. 2005, 21: 529-550. 10.1146/annurev.cellbio.21.012704.133214.
Berg van den B, Clemons WM, Collinson I, Modis Y, Hartmann E, Harrison SC: X-ray structure of a protein-conducting channel. Nature. 2004, 427: 36-44. 10.1038/nature02218.
Finke K, Plath K, Panzner S, Prehn S, Rapoport TA, Hartmann E: A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae. Embo J. 1996, 15: 1482-1494.
Hartmann E, Sommer T, Prehn S, Gorlich D, Jentsch S, Rapoport TA: Evolutionary conservation of components of the protein translocation complex. Nature. 1994, 367: 654-657. 10.1038/367654a0.
Panzner S, Dreier L, Hartmann E, Kostka S, Rapoport TA: Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p. Cell. 1995, 81: 561-570. 10.1016/0092-8674(95)90077-2.
Toikkanen J, Gatti E, Takei K, Saloheimo M, Olkkonen VM, Soderlund H: Yeast protein translocation complex: isolation of two genes SEB1 and SEB2 encoding proteins homologous to the Sec61 beta subunit. Yeast. 1996, 12: 425-438. 10.1002/(SICI)1097-0061(199604)12:5<425::AID-YEA924>3.0.CO;2-B.
Brodsky JL, Goeckeler J, Schekman R: BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum. Proc Natl Acad Sci USA. 1995, 92: 9643-9646. 10.1073/pnas.92.21.9643.
Young BP, Craven RA, Reid PJ, Willer M, Stirling CJ: Sec63p and Kar2p are required for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo. Embo J. 2001, 20: 262-271. 10.1093/emboj/20.1.262.
Romisch K: Endoplasmic reticulum-associated degradation. Annu Rev Cell Dev Biol. 2005, 21: 435-456. 10.1146/annurev.cellbio.21.012704.133250.
Vembar SS, Brodsky JL: One step at a time: endoplasmic reticulum-associated degradation. Nat Rev Mol Cell Biol. 2008, 9: 944-957. 10.1038/nrm2546.
Wilkinson BM, Tyson JR, Stirling CJ: Ssh1p determines the translocation and dislocation capacities of the yeast endoplasmic reticulum. Dev Cell. 2001, 1: 401-409. 10.1016/S1534-5807(01)00043-0.
Tsukazaki T, Mori H, Fukai S, Ishitani R, Mori T, Dohmae N: Conformational transition of Sec machinery inferred from bacterial SecYE structures. Nature. 2008, 455: 988-U72. 10.1038/nature07421.
Zimmer J, Nam YS, Rapoport TA: Structure of a complex of the ATPase SecA and the protein-translocation channel. Nature. 2008, 455: 936-U32. 10.1038/nature07335.
Kalies KU, Rapoport TA, Hartmann E: The beta subunit of the Sec61 complex facilitates cotranslational protein transport and interacts with the signal peptidase during translocation. J Cell Biol. 1998, 141: 887-894. 10.1083/jcb.141.4.887.
Levy R, Wiedmann M, Kreibich G: In vitro binding of ribosomes to the beta subunit of the Sec61p protein translocation complex. J Biol Chem. 2001, 276: 2340-2346. 10.1074/jbc.M004867200.
Helmers J, Schmidt D, Glavy JS, Blobel G, Schwartz T: The beta-subunit of the protein-conducting channel of the endoplasmic reticulum functions as the guanine nucleotide exchange factor for the beta-subunit of the signal recognition particle receptor. J Biol Chem. 2003, 278: 23686-23690. 10.1074/jbc.C300180200.
Feng D, Zhao X, Soromani C, Toikkanen J, Romisch K, Vembar SS: The transmembrane domain is sufficient for sbh1p function, its association with the sec61 complex, and interaction with rtn1p. J Biol Chem. 2007, 282: 30618-30628. 10.1074/jbc.M701840200.
Jiang Y, Cheng ZL, Mandon EC, Gilmore R: An interaction between the SRP receptor and the translocon is critical during cotranslational protein translocation. J Cell Biol. 2008, 180: 1149-1161.
Leroux A, Rokeach LA: Inter-species complementation of the translocon beta subunit requires only its transmembrane domain. PLoS ONE. 2008, 3: e3880-10.1371/journal.pone.0003880.
Esnault Y, Feldheim D, Blondel MO, Schekman R, Kepes F: SSS1 encodes a stabilizing component of the Sec61 subcomplex of the yeast protein translocation apparatus. J Biol Chem. 1994, 269: 27478-27485.
Lipschutz JH, Lingappa VR, Mostov KE: The exocyst affects protein synthesis by acting on the translocation machinery of the endoplasmic reticulum. J Biol Chem. 2003, 278: 20954-20960. 10.1074/jbc.M213210200.
Toikkanen JH, Miller KJ, Soderlund H, Jantti J, Keranen S: The beta subunit of the Sec61p endoplasmic reticulum translocon interacts with the exocyst complex in Saccharomyces cerevisiae. J Biol Chem. 2003, 278: 20946-20953. 10.1074/jbc.M213111200.
Guo W, Novick P: The exocyst meets the translocon: a regulatory circuit for secretion and protein synthesis?. Trends Cell Biol. 2004, 14: 61-63. 10.1016/j.tcb.2003.12.008.
Hu JJ, Shibata Y, Voss C, Shemesh T, Li ZL, Coughlin M: Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Science. 2008, 319: 1247-1250. 10.1126/science.1153634.
Shibata Y, Voss C, Rist JM, Hu J, Rapoport TA, Prinz WA: The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum. J Biol Chem. 2008, 283: 18892-18904. 10.1074/jbc.M800986200.
Voeltz GK, Prinz WA, Shibata Y, Rist JM, Rapoport TA: A class of membrane proteins shaping the tubular endoplasmic reticulum. Cell. 2006, 124: 573-586. 10.1016/j.cell.2005.11.047.
De Craene JO, Coleman J, Estrada dM, Pypaert M, Anderson S, Yates JR: Rtn1p is involved in structuring the cortical endoplasmic reticulum. Mol Biol Cell. 2006, 17: 3009-3020. 10.1091/mbc.E06-01-0080.
Sherman F: Getting started with yeast. Guide to yeast genetics and molecular biology. Edited by: Guthrie C, Fink GR. 1991, Academic Press; San Diego, 3-21.
Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H: A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast. 2004, 21: 947-962. 10.1002/yea.1142.
Gietz RD, Schiestl RH: Applications of High-Efficiency Lithium-Acetate Transformation of Intact Yeast-Cells Using Single-Stranded Nucleic-Acids As Carrier. Yeast. 1991, 7: 253-263. 10.1002/yea.320070307.
Vernet T, Dignard D, Thomas DY: A family of yeast expression vectors containing the phage f1 intergenic region. Gene. 1987, 52: 225-233. 10.1016/0378-1119(87)90049-7.
Cronan JE: Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins. J Biol Chem. 1990, 265: 10327-10333.
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B: A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking. Mol Cell Proteomics. 2006, 5: 737-748.
Mumberg D, Muller R, Funk M: Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 1995, 156: 119-122. 10.1016/0378-1119(95)00037-7.
Mason N, Ciufo LF, Brown JD: Elongation arrest is a physiologically important function of signal recognition particle. Embo J. 2000, 19: 4164-4174. 10.1093/emboj/19.15.4164.
Menetret JF, Hegde RS, Aguiar M, Gygi SP, Park E, Rapoport TA: Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome. Structure. 2008, 16: 1126-1137. 10.1016/j.str.2008.05.003.
Johnsson N, Varshavsky A: Ubiquitin-Assisted Dissection of Protein-Transport Across Membranes. Embo J. 1994, 13: 2686-2698.
Chavan M, Yan A, Lennarz WJ: Subunits of the translocon interact with components of the oligosaccharyl transferase complex. J Biol Chem. 2005, 280: 22917-22924. 10.1074/jbc.M502858200.
Ishikawa H, Barber GN: STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature. 2008, 455: 674-U74. 10.1038/nature07317.
Chien YC, Kim S, Bumeister R, Loo YM, Kwon SW, Johnson CL: RaIB GTPase-mediated activation of the I kappa B family kinase TBK1 couples innate immune signaling to tumor cell survival. Cell. 2006, 127: 157-170. 10.1016/j.cell.2006.08.034.
Jeremy Brown, Nils Johnsson, Peter Kaiser, Michael Knop, and Peter Novick are acknowledged for providing us with strains and plasmids. Michael Knop is also thanked for providing the error-prone PCR protocol. Karin Römisch, Sirkka Keränen and members of Jäntti group are thanked for discussions and comments on the manuscript. Anna-Liisa Nyfors is thanked for for excellent technical assistance. This work was financially supported by the Academy of Finland Grants 124249 and 211171 to JJ, Magnus Ehrnrooth Foundation, and the Institute of Biotechnology.
XZ and JJ planned and XZ performed the experiments. JJ drafted the manuscript. Both authors read, edited and approved the final manuscript.
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Zhao, X., Jäntti, J. Functional characterization of the trans-membrane domain interactions of the Sec61 protein translocation complex beta-subunit. BMC Cell Biol 10, 76 (2009). https://doi.org/10.1186/1471-2121-10-76
- Protein Translocation
- High Resolution Structure
- Sec61 Complex
- V57G Mutation
- Exocyst Complex