- Research article
- Open Access
Phospholipase Cδ regulates germination of Dictyostelium spores
© Van Dijken and Van Haastert; licensee BioMed Central Ltd. 2001
- Received: 9 November 2001
- Accepted: 5 December 2001
- Published: 5 December 2001
Many eukaryotes, including plants and fungi make spores that resist severe environmental stress. The micro-organism Dictyostelium contains a single phospholipase C gene (PLC); deletion of the gene has no effect on growth, cell movement and differentiation. In this report we show that PLC is essential to sense the environment of food-activated spores.
Plc-null spores germinate at alkaline pH, reduced temperature or increased osmolarity, conditions at which the emerging amoebae can not grow. In contrast, food-activated wild-type spores return to dormancy till conditions in the environment allow growth. The analysis of inositol 1,4,5-trisphosphate (IP3) levels and the effect of added IP3 uncover an unexpected mechanism how PLC regulates spore germination: i) deletion of PLC induces the enhanced activity of an IP5 phosphatase leading to high IP3 levels in plc-null cells; ii) in wild-type spores unfavourable conditions inhibit PLC leading to a reduction of IP3 levels; addition of exogenous IP3 to wild-type spores induces germination at unfavourable conditions; iii) in plc-null spores IP3 levels remain high, also at unfavourable environmental conditions.
The results imply that environmental conditions regulate PLC activity and that IP3 induces spore germination; the uncontrolled germination of plc-null spores is not due to a lack of PLC activity but to the constitutive activation of an alternative IP3-forming pathway.
- Fruiting Body
- Spore Germination
- Dictyostelium Cell
- Severe Environmental Stress
- Induce Spore Germination
Many extracellular signals activate inositide-specific phospholipase C (PLC) thereby producing the second messengers Ins(1,4,5)P3 and diacylglycerol . Three types of PLC are known, PLC-β, PLC-γ and PLC-δ which are regulated by G-proteins, receptor tyrosine kinases, and Ca2+, respectively . Animals such as human and rat, but also C. elegans, Artemia, Loligo forbesi and Drosophila possess all three PLC isoforms [3–6]. However, in non-animals exclusively PLC-δ has been identified, e.g. in soybean  and catfish , and the lower eukaryotes Dictyostelium discoideum, Saccharomyces cerevisiae[10–12] and Schizosaccharomyces pombe. This phylogenetic distribution of PLC isozymes is in accordance with the deduced three dimensional structure, suggesting that PLC-δ is the ancient isoform to which specific domains were added in PLC-β and PLC-γ [14, 15].
Dictyostelium cells live in the soil where they feed on bacteria. When the bacteria become scarce, starvation induces the expression of a cAMP sensory system that mediates cell aggregation. A fruiting body is formed consisting of spores embedded in a slime droplet on top of a stalk. When nutrients are available, spores germinate and amoebae search for bacteria by chemotaxis. Spores are relatively safe to environmental stress; no intake of food is required. Furthermore, spores resist extreme temperatures, humidity and pH, and they can pass the digestive track of birds and nematodes .
Previously, a Dictyostelium mutant was constructed in which the single PLC-δ gene was disrupted resulting in cells without detectable PLC activity . Besides the unexpected finding that these cells contained near-normal IP3 levels due to an alternative route of IP3 synthesis , they also showed no abnormal phenotype. At optimal laboratory conditions neither growth nor development were affected . However, optimal conditions are not likely to occur for a very long period in the habitat of Dictyostelium, which is the upper layer of the soil. Therefore we analyzed the survival of the species at suboptimal conditions.
Normal stress resistance in plc-null cells
A Dictyostelium cell lacking the single PLCδ gene shows no abberrant phenotype at laboratory conditions. Therefore we measured the kinetics and dose-dependencies for survival of the amoebae at various stress conditions, including temperature, pH, osmolarity, and removal of extracellular Ca2+. We never observed a significant differences between plc-null and control cells (data not shown), suggesting that Dd-PLCδ is not essential to protect the amoebae. This is in strong contrast to another second messenger enzyme, guanylyl cyclase, which protects Dictyostelium cells against osmotic stress .
Normal differentiation in plc-null cells
A common theme in species that have only the PLCδ isoform is the formation of spores or seeds, which can survive extreme environmental conditions. Sporulation to survive stress comes to an evolutionary advantage when germination of spores occurs only at conditions that allow growth of the emerging organism. We investigated the role of PLC in sporulation and germination. During Dictyostelium development about two-thirds of the cells differentiates into viable spores, whereas one-third develops into dead stalk cells. We observed that the proportioning of stalk and spore cells is not different in plc-null fruiting bodies (data not shown). Next, plc-null cells with a Neo marker were mixed 1:1 with wild-type cells containing a Bsr marker conferring resistance to the antibiotics G418 and blasticidin, respectively. Spores were isolated from the resulting fruiting bodies, and inoculated in microtiter plates at low density such that about 40% of the cultures showed growth. Subsequently cultures were transferred to media with G418 or blasticidin, allowing growth of plc-null and wild-type cells, respectively. In three independent experiments we observed that from a total of 101 cultures, 29 did grow in G418, 31 grew in blasticidin, whereas 41 showed growth in both G418 and blasticidin. This experiment demonstrates that the spores have essentially the same plc-null/wild-type ratio as the 1:1 mixture of cells from which they were derived, suggesting that Dd-PLCδ does not play a role in the formation of spores.
Aberrant spore germination in plc-null cells
Spore germination at different environmental conditions
50% germination (hours)
16.9 ± 0.6
38 ± 7
7.1 ± 2.0
17.4 ± 2.6
5.1 ± 0.4
7.0 ± 0.5
4.3 ± 1.1
4.3 ± 0.3
4.2 ± 0.2
4.3 ± 0.3
4.2 ± 0.2
5.5 ± 1.1
4.6 ± 0.4
10.0 ± 0.7
5.2 ± 0.9
17.8 ± 1.2
6.3 ± 1.4
30.9 ± 3.1
9.0 ± 1.0
127 ± 21
88 ± 18
19.7 ± 2.6
16.8 ± 2.6
7.6 ± 2.4
9.4 ± 3.1
3.6 ± 1.6
4.2 ± 1.7
4.3 ± 0.3
4.2 ± 0.2
4.6 ± 1.4
5.0 ± 1.3
8.2 ± 0.9
5.7 ± 0.8
20.6 ± 3.4
9.0 ± 1.1
Osmotic pressure and pH of the medium are other environmental factors known to affect spore germination. Germination of wild-type spores is more strongly inhibited by sucrose than germination of plc-null spores (Figure 1B). Addition of 0.3 M sucrose inhibits wild-type germination 7-fold while germination of plc-null spores is retarded only 2-fold (Table 1). At acidic pH, spore germination is inhibited about 25-fold at pH 4.7, 5-fold at pH 5.2, and about 2-fold at the mildly acidic pH of 5.5 (Table 1); no statistical significant differences can be observed between germination of wild-type and plc-null spores. At a slightly alkaline pH up to pH 7.8 spore germination is not strongly affected if compared to the optimal pH, but at more alkaline pH germination is inhibited. As with temperature and osmolarity, germination is significantly more affected for wild-type than for plc-null spores.
IP3 levels during spore germination
A simple model can explain the effect of PLC disruption on spore germination: unfavourable conditions activate PLC resulting in a return to dormancy of food-activated spores; in plc-null cells PLC can not be activated, so activated spores can not return to dormancy. However, we have observed that amoebae lacking PLC activity have near-normal IP3 levels due to an alternative route of IP3 formation, obtained from the degradation of IP5. Therefore, we have measured IP3 levels in wild-type and plc-null spores germinating at 22°C and 16°C.
A simple biochemical hypothesis could explain the results: unfavourable conditions activate PLC resulting in IP3 formation that inhibits germination; plc-null cells lack the ability to synthesize IP3 by which activated spores can not return to dormancy. This hypothesis appears to be incorrect. Firstly, IP3 levels of plc-null spores are 50% higher than IP3 levels of wild-type spores. Secondly, in wild-type cells unfavourable conditions inhibit rather than stimulate IP3 formation. Thirdly, at unfavourable conditions exogenously added IP3 does not inhibit germination of plc-null spores, but promote germination of wild-type spores. Finally, Lydan and Cotter  have demonstrated that in wild-type cells IP3 acts synergistically with autoactivator to stimulate germination of saponin treated spores, and that EGTA will inhibit swelling of autoactivating spores.
Dictyostelium as well as mammalian cells contain two routes for IP3 formation, PLC-mediated hydrolysis of the phospholipid PIP2, and degradation of a specific IP5-isomer, Ins(1,3,4,5,6)P5, by the enzyme MIPP. Non-equilibrium labelling experiments with [3H]inositol demonstrate that in wild-type Dictyostelium cells at least 90% of IP3 is produced by PLC, whereas in plc-null cells all IP3 is derived from IP5[18, 25]. In addition, our observation of depleted IP5 levels in plc-null cells, strongly suggesting that the IP3 pathway from IP5 is activated in plc-null cells .
All experiments are consistent with a more complex hypothesis for the regulation of spore germination in Dictyostelium (Figure 5). Plc-null spores contain an activated route of IP3 formation from IP5, leading to enhanced IP3 levels in comparison to wild-type spores. Activated spores will germinate when IP3 levels remain high, but return to dormancy at reduced IP3 levels. Unfavourable conditions inhibit PLC leading to reduced IP3 levels in food-activated wild-type spores. Apparently IP3 formation from IP5 is not inhibited, IP3 levels do not decrease, and spore germination continues at unfavourable conditions in plc-null spores. Finally, exogenously added IP3 promotes germination of wild-type spores at conditions that induce a depletion of intracellular IP3 and a return to dormancy. The depletion of intracellular IP3 in wild-type cells at reduced temperature is transient. Thus, spore germination is inhibited although IP3 levels have returned to the high basal levels. Apparently, the transient reduction of IP3 levels during spore activation has modified the sporulation proces.
Many eukaryotes, including plants and fungi make spores that resist severe environmental stress. These spores allow species to preserve their genes even when the individuals that have produced the spores are dead. In contrast, mammals can only transmit their genes while the individual is still living. Gene maintenance through spores requires strict regulation of spore germination, which should only occur when the environment can support growth of the emerging organism. It is clear that a return to dormancy of food activated spores at environmental conditions that can not support growth of the emerging organism is of great evolutionary importance. The lack of Dd-PLCδ is a severe disadvantage in the wild, even though under normal laboratory conditions no phenotype of plc-null cells can be observed. It is not known whether this function of PLCδ in Dictyostelium is restricted to spore-forming organisms possessing only the δ-isoform of PLC, and that in higher organisms PLCδ fulfils a similar control role, distinct from the functions of the additional PLCβ and PLCγ.
By analyzing spore germination and IP3 levels in wild-type and plc-null strains we conclude that harmful environmental conditions inhibit PLC activity and that the reduced IP3 levels prevent spore germination. In plc-null strains, an alternative pathway for IP3 formation is induced that is not inhibited by harmful environmental conditions. As a consequence, IP3 levels are not inhibited and plc-null spores germinate at environmental conditions where the emerging amoebae can not survive.
Two sets of Dictyostelium cells were used. HD10 (plc-null cells) and HD11 (wild-type control for HD10) are G418 resistant clones in an AX3 background . Clone 1.19 (plc-null cells) and 0-mut (PLC expressed in 1.19 using an actin 15 promotor) are transformants in a DH1 background . The experiments presented in this report were performed with the combination HD10/HD11; the experiments of figures 1 and 2 were reproduced with the combination 1.19/0-mut.
Spore germination and cell growth
Spores were isolated from 1 to 3 days old fruiting bodies. After treatment with 0.5% NP-40 for 3 minutes to kill remaining amoebae, spores were washed three times with water, and inoculated at a final density of 1.5 × 106 spores per ml in flasks containing 10 ml medium as indicated. The flasks were shaken (150 rpm) at 22°C or 16°C. At various time intervals the spores were observed by phase contrast microscopy and scored for unswollen spores and amoebae. Germination is defined as the fraction of spores that have emerged as amoebae.
Cell growth was measured in medium as indicated in figure 2 using freshly growing cells from a culture at 22°C in HG5 medium at a density of 2 × 106 cells/ml.
Determination of IP3 levels
Two days old spores were incubated at a density of 1.5 × 108/ml in HG5 medium at 22°C or 16°C. At the times indicated in figure 3, 20 μl of the suspension was added to 20 μl ice-cold 3.5% (v/v) perchloric acid. After incubation in a sonication bath for 15 min, the lysates were neutralized with 10 μl KHCO3 (50% saturated at 22°C). IP3 levels were measured in the extracts using an isotope dilution assay .
- Berridge MJ, Irvine RF: Inositol phosphates and cell signalling. Nature. 1989, 341: 197-205. 10.1038/341197a0.View ArticlePubMedGoogle Scholar
- Rhee SG, Bae YS: Regulation of phosphoinositide-specific phospholipase C isozymes. J. Biol. Chem. 1997, 272: 15045-15048. 10.1074/jbc.272.24.15045.View ArticlePubMedGoogle Scholar
- Bloomquist BT, Shortridge RD, Schneuwly S, Perdew M, Montell C, Steller H, Rubin G, Pak WL: Isolation of a putative phospholipase C of Drosophila, norpA, and its role in phototransduction. Cell. 1988, 54: 723-733.View ArticlePubMedGoogle Scholar
- Shortridge RD, Yoon J, Lending C, Bloomquist BT, Perdew MH, Pak WL: A Drosophia phospholipase C gene that is expressed in the central nervous system. J. Biol. Chem. 1991, 266: 12474-12480.PubMedGoogle Scholar
- Su X, Chen F, Hokin LE: Cloning and expression of a novel, highly truncated phosphoinositide-specific phospholipase C cDNA from embryos of the brine shrimp, Artemia. J. Biol. Chem. 1994, 269: 12925-12931.PubMedGoogle Scholar
- Carne A, McGregor RA, Bhatia J, Sivaprasadarao A, Keen JN, Davies A, Findlay JBC: A â-subclass phosphatidylinositol-specific phospholipase C from squid (Loligo forbesi) photoreceptors exhibiting a truncated C-terminus. FEBS Lett. 1995, 372: 243-248. 10.1016/0014-5793(95)00936-4.View ArticlePubMedGoogle Scholar
- Shi J, Gonzales RA, Bhattacharyya MK: Characterization of a plasma membrane-associated phosphoinositide-specific phospholipase C from soybean. Plant J. 1995, 8: 381-390. 10.1046/j.1365-313X.1995.08030381.x.View ArticlePubMedGoogle Scholar
- Abogadie FC, Bruch RC, Wurzburger R, Margolis FL, Farbman AL: Molecular cloning of a phosphoinositide-specific phospholipase C from catfish olfactory rosettes. Brain Res. Mol. Brain Res. 1995, 31: 10-16. 10.1016/0169-328X(95)00030-V.View ArticlePubMedGoogle Scholar
- Drayer AL, Van Haastert PJM: Molecular cloning and expression of a phosphoinositide-specific phospholipase C of Dictyostelium discoideum. J. Biol. Chem. 1992, 267: 18387-18392.PubMedGoogle Scholar
- Yoko-o T, Matsui Y, Yagisawa H, Nojima H, Uno I, Toh-e A: The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth. Proc. Natl. Acad. Sci. USA. 1993, 90: 1804-1808.PubMed CentralView ArticlePubMedGoogle Scholar
- Flick JS, Thorner J: Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae. Mol. Cell. Biol. 1993, 13: 5861-5876.PubMed CentralView ArticlePubMedGoogle Scholar
- Payne WE, Fitzgerald-Hayes M: A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation. Mol. Cell. Biol. 1993, 13: 4351-4364.PubMed CentralView ArticlePubMedGoogle Scholar
- Andoh T, Yoko-o T, Matsui Y, Toh-e A: Molecular cloning of the plc1+ gene of Schizosaccharomyces pombe, which encodes a putative phosphoinositide-specific phospholipase C. Yeast. 1995, 11: 179-185.View ArticlePubMedGoogle Scholar
- Essen L-O, Perisic O, Cheung R, Katan M, Williams RL: Crystal structure of a mammalian phosphoinositide-specific phospholipase Cdelta. Nature. 1996, 380: 595-602. 10.1038/380595a0.View ArticlePubMedGoogle Scholar
- Williams RL, Katan M: Structural views of phosphoinositide-specific phospholipase C: signalling the way ahead. Structure. 1996, 4: 1387-1394.View ArticlePubMedGoogle Scholar
- Kessin RH, Gunderson GG, Zaydfudim V, Grimson M, Blanton RH: How cellular slime molds evade nematodes. Proc. Natl. Acad. Sci. USA. 1996, 93: 4857-4861. 10.1073/pnas.93.10.4857.PubMed CentralView ArticlePubMedGoogle Scholar
- Drayer AL, Van der Kaay J, Mayr GW, Van Haastert PJM: Role of phospholipase C in Dictyostelium: formation of inositol 1,4,5-trisphosphate and normal development in cells lacking phospholipase C activity. EMBO J. 1994, 13: 1601-1609.PubMed CentralPubMedGoogle Scholar
- Van Dijken P, de Haas J-R, Craxton A, Erneux C, Shears SB, Van Haastert PJM: A novel, phospholipase C-independent pathway of inositol 1,4,5-trisphosphate formation in Dictyostelium and rat liver. J. Biol. Chem. 1995, 270: 29724-29731. 10.1074/jbc.270.50.29724.View ArticlePubMedGoogle Scholar
- Kuwayama H, Ecke M, Gerisch G, Van Haastert PJM: Protection against osmotic stress by cGMP-mediated myosin phosphorylation. Science. 1996, 271: 207-209.View ArticlePubMedGoogle Scholar
- Bominaar AA, Kesbeke F, Van Haastert PJM: Phospholipase C in Dictyostelium discoideum (1994) ; Cyclic AMP surface receptor and G-protein-regulated activity in vitro. Biochem. J. 1994, 297: 181-187.PubMed CentralView ArticlePubMedGoogle Scholar
- Cotter DA, Raper KB: Spore germination in Dictyostelium discoideum. Proc. Natl. Acad. Sci. USA. 1966, 56: 880-887.PubMed CentralView ArticlePubMedGoogle Scholar
- Van Es S, Virdy KJ, Pitt GS, Meima M, Sands TW, Devreotes PN, Cotter DA, Schaap P: Adenylyl cyclase G, an osmosensor controlling germination of Dictyostelium spores. J. Biol. Chem. 1996, 271: 23623-23625. 10.1074/jbc.271.39.23623.View ArticlePubMedGoogle Scholar
- Cotter DA, Sands TW, Virdy KJ, North MJ, Klein G, Sartre M: Patterning of development in Dictyostelium dicoideum: factors regulating growth, differentiation, spore dormancy, and germination. Biochem. Cell. Biol. 1992, 70: 892-919.View ArticlePubMedGoogle Scholar
- Lydan MA, Cotter DA: The role of Ca2+ during spore germination in Dictyostelium: Autoactivation is mediated by the mobilization of Ca2+ while amoebal emergance requires entry of external Ca2+. J. Cell Sci. 1995, 108: 1921-1930.PubMedGoogle Scholar
- Van Haastert PJM, Van Dijken P: Biochemistry and genetics of inositol phosphate metabolism in Dictyostelium. FEBS Lett. 1997, 410: 29-43. 10.1016/S0014-5793(97)00358-X.View ArticleGoogle Scholar
- Drayer AL, Meima ME, Derks MWM, Tuik R, Van Haastert PJM: Mutation of an EF-hand Ca2+-binding motif in phospholipase C of Dictyostelium discoideum: inhibition of activity but no effect on Ca2+-dependence. Biochem. J. 1995, 311: 505-510.PubMed CentralView ArticlePubMedGoogle Scholar
- Van Haastert PJM: Determination of inositol 1,4,5-trisphosphate levels in Dictyostelium by isotope dilution assay. Anal. Biochem. 1989, 177: 115-119.View ArticlePubMedGoogle Scholar
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